Integra VIAFLO 4011 VIAFLO 4012 Operating Instructions Manual
Electronic/adjustable tip spacing pipettes
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Summary of Contents for Integra VIAFLO 4011 VIAFLO 4012
- Page 1 VIAFLO Electronic Pipettes Operating instructions VOYAGER Adjustable Tip Spacing Pipettes 161950_V08...
- Page 2 Declaration of conformity INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the devices Description Models VIAFLO Pipettes 4011, 4012, 4013, 4014, 4015, 4016, 4621, 4622, 4623, 4624, 4626, 4631, 4632, 4633, 4634, 4636, 4641, 4642, 4646...
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Page 3: Table Of Contents
3.2.2 Charging the battery with the mains adapter ......16 3.3 Exchanging the battery..............17 3.4 Toolbox - adapt your pipette............17 3.4.1 Preferences.................18 3.4.2 Calibration & Service ............20 3.4.3 Communications ..............21 3.4.4 Device Information ..............22 3.4.5 Language ................22 3.4.6 Write protect................23 www.integra-biosciences.com... - Page 4 5.2.8 Multi Aspirate mode ............42 5.2.9 Sample Dilute/Mix mode .............43 5.2.10 Serial Dilution mode............44 5.3 Custom step-based programming mode ........45 5.3.1 Create a custom program ...........45 5.3.2 Modify existing programs ............47 5.3.3 Example of custom mode ...........48 INTEGRA Biosciences AG...
- Page 5 6.2.1 VIAFLO single channel lower end........49 6.2.2 VIAFLO multichannel pipettes ..........53 6.3 Sterilization ..................54 6.3.1 Autoclaving the disassembled components......54 6.4 Servicing..................55 6.4.1 Shipping to INTEGRA Biosciences........55 6.4.2 Changing O-rings of tip fittings..........55 6.4.3 Lubrication ................56 6.5 Equipment disposal ................56 Chapter 7 Calibration 7.1 Definitions..................57...
- Page 6 © 2018 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
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Page 7: Chapter 1 Introduction
IVD setting is under the sole responsibility of the user. If the VIAFLO/VOYAGER Electronic Pipettes are used in a manner not specified by INTEGRA Biosciences, the protection provided by the VIAFLO/VOYAGER Electronic Pipettes may be impaired. -
Page 8: Safety Notes
ARNING • Use only an original INTEGRA Li-ion battery (#4205) and charging device. • Old Li-ion batteries may cause a safety risk. We recommend to replace the battery after 3 years of use. - Page 9 Regardless of the listed safety notes, additional applicable regulations and guidelines of trade associations, health authorities, trade supervisory offices, etc. must be observed. Please visit our website www.integra-biosciences.com on a regular basis for up to date information regarding REACH classified chemicals contained in our products.
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Page 10: Description Of The Device
• O-ring removal tool (300 μl and 1250 μl volume ranges only) • Certificate of Performance AUTION Verify the scope of delivery when unpacking the device and check for potential transportation damage. Do not operate a device that is damaged, instead contact your local dealer. INTEGRA Biosciences AG... -
Page 11: Overview Of The Viaflo/Voyager Electronic Pipettes
5 Left and Right Arrow Buttons, for selections 6 PURGE Button, to empty tips 7 RUN Key, to start operations 8 Tip Ejector 9 Finger Hook, facilitates easy operation 10 Volume Indicator Label, color matches GripTip box insert. 11 Ejector Sleeve 12 Tip Fitting www.integra-biosciences.com... -
Page 12: Back View
Description of the device 2.2.2 Back view 13 Reset Button 14 Power Connector 15 Charging Stand Interface 16 Battery 2.2.3 Display The Display shows all pipetting options. Battery Life Indicator Current mode Instruction Active step Volumes Options INTEGRA Biosciences AG... -
Page 13: Touch Wheel
Press and release the RUN Key (7) to initiate aspiration, dispense, mix, purge, and special pipetting operations. This button is centrally located for left- or right-handed actuation. During dispense, you can press and hold the RUN Key to perform a two-step blowout, see “4.3.2 Blowout modes” on page 25. www.integra-biosciences.com... -
Page 14: Tip Ejector
The programs stored in memory are maintained. Once pressed, the startup screen will be displayed. Press any key to continue and allow the instrument to initialize and home. The routine ends by displaying the Main Menu. INTEGRA Biosciences AG... -
Page 15: Chapter 3 Installation
• Two static and one blinking bar on the right: Pipette is recharging and battery is 80% charged. • Three static bars: Battery is fully charged. AUTION Use only the approved INTEGRA battery, mains adapter or charging stand, see (“9.1 Accessories” on page 71). Use of an incompatible power transformer can damage the pipette. www.integra-biosciences.com... -
Page 16: Charging The Battery On A Stand
“4.1 Turn on/off the device” on page 24), but it will continue to show the battery life indicator. When the Standby Time is reached, the display is turned off. When the Turn Off Time is reached, the instrument will shut off. INTEGRA Biosciences AG... -
Page 17: Exchanging The Battery
After exchanging the battery, a protective switch is active. The pipette can only be started after connecting it to the mains power supply. ARNING INTEGRA VIAFLO/VOYAGER Electronic Pipettes use Li-ion batteries, see “1.3 Safety notes” on page 8. Toolbox - adapt your pipette The Toolbox provides options to adapt the device to appropriate applications, setting personal preferences, calibration, computer connectivity and storing device information. -
Page 18: Preferences
Select a function to be hidden from the main menu (Off) / (On/Off) and press OK, e.g. ASSIST, Pipet, Repeat Dispense, Sample Dilute, Pipet/Mix, Tip spacing. Touch Adjust your Touch Wheel sensitivity. Press to save. Low, Medium, Wheel High INTEGRA Biosciences AG... - Page 19 Extending the volume range is not recommended. Full functionality and specified accuracy/precision cannot be guaranteed. The default values of the speed table are optimized for the pipette motor. Changing the values may cause a more noisy operation of the pipette but does not do harm to the pipette. www.integra-biosciences.com...
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Page 20: Calibration & Service
• Reset: Resets the timer to the defined calibration interval. Press to enable. Press to save. Service Displays notes of any service that took place on the History pipette listed newest entry first. INTEGRA Biosciences AG... -
Page 21: Communications
VIALINK is a pipette management software for the PC. It allows the user to upload/ download custom programs, images, firmware updates and service history to and from VIAFLO/VOYAGER Electronic Pipettes. It can be downloaded from the INTEGRA website in the product section. A detailed description of the software, along with the operating instructions, can be found on the website as well. -
Page 22: Device Information
It lists the standards the pipettes comply with. 3.4.5 Language Language Description Language You can choose the language in which all screens are displayed. Scroll to the desired language, press OK and to Save. INTEGRA Biosciences AG... -
Page 23: Write Protect
OK. Press to save the password. The password must be entered before you can access the write protect menu. Keep the password in a safe place. Should you lose your password, contact INTEGRA Biosciences to retrieve your password. www.integra-biosciences.com... -
Page 24: Chapter 4 Operation
Pipettes always use appropriate GripTips, see “9.2 Consumables” on page 71. The unique GripTip system of INTEGRA pipettes reduces attachment and ejection forces, ensures a perfect fitting to prevent the tips from falling off and to provide a perfect seal. -
Page 25: Start Pipetting
• Automatic two-step blowout: Perform a two-step blowout to manually delay the blowin: - Press and hold the RUN Key to start dispense with blowout. - Remove the tips from the target vessel. - Release the RUN Key to start blowin. www.integra-biosciences.com... -
Page 26: Recommendations For Pipetting
4.3.3 Recommendations for pipetting INTEGRA Biosciences recommends the following techniques for enhancing pipetting results. These techniques are consistent with ISO standard 8655-2. • It is best to immerse the GripTips just enough in liquid (2–3 mm) to allow the desired volume to be aspirated. -
Page 27: Pipetting Options And Settings
• When in other modes, select the Edit option and press OK. The steps with volumes to be adjusted are displayed. Use the Touch Wheel to highlight the desired volume and press Favorites to display the list of favorite volumes. www.integra-biosciences.com... -
Page 28: Speed Selection
Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting. To dispense liquids with low viscosity and high vapor pressure, such as ethanol, use relative fast pipetting speeds and avoid prolonged pauses for aspiration. INTEGRA Biosciences AG... -
Page 29: Pace
Select a desired value. Press OK and then press to save your setting(s). 4.4.6 Help The Help information available for each mode describes the mode operation. While in the Main menu, highlight a pipet mode, then press to select the Help option. www.integra-biosciences.com... -
Page 30: Voyager Tip Spacing
Middle position, and 9.0 mm is the Last position. The current position is highlighted. Press or to move the tips to the next position. The tip spacing can be changed at any step in the program. INTEGRA Biosciences AG... -
Page 31: Homing The Tips
Every time the pipette is turned on, the tip spacing motor homes and moves to the first tip spacing position. You may also home the tip spacing motor when needed. To do so, press Home Tips on the Tip Spacing menu. www.integra-biosciences.com... -
Page 32: Troubleshooting/Faq
• Touch off was not per- • Perform a touch-off (mandatory formed. in Repeat Dispense and Vari- able Dispense mode). Software does not • Software frozen. • Press Reset Button on back of react. the pipette. INTEGRA Biosciences AG... -
Page 33: Electronic
• Indicates possible motor fail- tance. ure. Tip spacing motor • Tip spacing motor drive not • Reset the pipette. not working. initialized. • Turn pipette off. Unplug battery for about 5 seconds. Turn pipette www.integra-biosciences.com... -
Page 34: Pipetting Modes
Allows aspirating a transfer volume followed by a mix. Rows and Mix Cycles are tracked on the display. Custom Programs Allows to create and store of up to 40 multi-stepped pipetting protocols. Press the OK to access the pipetting mode and to start defining parameters. INTEGRA Biosciences AG... -
Page 35: Detailed Description Of Pipetting Modes
• With the tip(s) in the destination plate, press and hold the RUN Key to execute the dis- pense and perform a two-step blowout, see “4.3.2 Blowout modes” on page 25. • When the tips are removed from the target plate, release the RUN Key. www.integra-biosciences.com... -
Page 36: Repeat Dispense Mode
- If reuse of post-dispense is active, you can start the next repeat dispense cycle with aspirating liquid to the post-dispense in the tip. To finish the repeat dispense cycle, press Purge. INTEGRA Biosciences AG... -
Page 37: Sample Dilute Mode
Operation: • Press and release the RUN Key to initiate each aspiration (remove tips from liquid for air-gap aspiration). • Press and hold the RUN Key to perform a two-step blowout. The entire tip contents will be dispensed together. www.integra-biosciences.com... -
Page 38: Pipet/Mix Mode
• Press and release the RUN Key to dispense. Mixing occurs automatically after the dispense step. • Upon completing the desired number of mixes, a blowout is initiated automatically prompting you to remove the tip(s) from the liquid and press RUN to complete the blowout. INTEGRA Biosciences AG... -
Page 39: Manual Pipet Mode
The notation on the display changes between (Aspirate) and (Dispense). • Titrations can be performed by dispensing in this mode. The volume remaining in the tip(s) is always actively displayed. Use slower pipetting speeds (1–5) for better control and resolution. www.integra-biosciences.com... -
Page 40: Reverse Pipet Mode
• If reuse of post-dispense is active, you can start the next reverse pipet cycle with aspi- rating liquid to the post-dispense in the tip. To finish the reverse pipet cycle, press Purge. INTEGRA Biosciences AG... -
Page 41: Variable Dispense Mode
Last Dispense. This aliquot contains the accumulated error from all prior dispenses. You can choose to use this Last Dispense or discard it. • During the Last dispense, press and hold the RUN Key to perform a two-step blowout. www.integra-biosciences.com... -
Page 42: Multi Aspirate Mode
• With the tip(s) in liquid, press and release the RUN Key to initiate the first aspiration volume. Again in liquid, press and release the RUN Key to initiate the second aspira- tion volume, etc. • Press and hold the RUN Key to start Dispense and perform a two-step blowout. INTEGRA Biosciences AG... -
Page 43: Sample Dilute/Mix Mode
• Press and release the RUN Key to dispense the entire tip contents and begin the mix- ing routine. Upon completing the desired number of mixes, a blowout occurs automat- ically. Remove tips from liquid and press and release the RUN Key to complete the blowout. www.integra-biosciences.com... -
Page 44: 5.2.10 Serial Dilution Mode
RUN Key to start the dispense and mix sequence. Proceed with the rest of the rows. • Rows (first number) and Mix Cycles (second number) are tracked on the display. Mix Cycles are shown in red when mixing. A green dot on the row number indicates the active program step. INTEGRA Biosciences AG... -
Page 45: Custom Step-Based Programming Mode
When starting with an “Aspirate” step followed by a “Mix” step, the tips contain the aspirate volume after completing the last mix cycle. When starting with a “Mix” step, the tips are emptied upon completion of the last mixing cycle. www.integra-biosciences.com... - Page 46 Performs a blowout. A blowout needs to be performed after the last dispense to expel residual liquid. Note: When using a standard “Dispense” step or “Purge”, a blowout/ blowin is performed automatically to empty the tips and does not need to be programmed. INTEGRA Biosciences AG...
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Page 47: Modify Existing Programs
To insert a new step, press Edit, select New Step and press OK. Use the Touch Wheel to select the position where a new step should be inserted and press OK. Select an operation and press OK. Press Save to return to the list of Custom programs. www.integra-biosciences.com... -
Page 48: Example Of Custom Mode
Purge does not need to be programmed. The residual liquid is dispensed into the waste container. Press and hold RUN Key until liquid is purged and tips are removed from the liquid (two-step blowout). For a detailed description see “4.3.2 Blowout modes” on page 25. INTEGRA Biosciences AG... -
Page 49: Chapter 6 Maintenance
2) Turn off power and unplug the mains adapter. 3) Unscrew counterclockwise to remove the Cylinder Assembly (a) from the pipette. Slide the Cylinder Assembly down from the pipette body. This exposes the Piston Assembly (b, models 12.5 µl–300 µl) or Piston (model 1250 µl). www.integra-biosciences.com... - Page 50 4) Slide the following components from the Piston (h): • O-Ring (black, g) and Seal (white, f) Assembly • Seal Retainer Spring (d) • Seal Retainer (black or white, c) Set these components aside or place them in an autoclave pouch. 125 µl 300 µl INTEGRA Biosciences AG...
- Page 51 “9.2 Consumables” on page 71. 1) Lightly lubricate the Piston, O-Rings and Seals (see “6.4.3 Lubrication” on page 56). Models 12.5 µl–1250 µl: 2) Position the Piston (h) at the small magnet to connect the Piston to the upper part of the pipette. www.integra-biosciences.com...
- Page 52 9) Slide the Cylinder Assembly (a) over the Piston (h, models 12.5 µl–1250 µl). Screw clockwise to attach the Cylinder Assembly to the body of the pipette. Perform a leak test (see “7.3.5 Leak test” on page 59) and validate pipetting volumes after reassembly. INTEGRA Biosciences AG...
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Page 53: Viaflo Multichannel Pipettes
Disengage the ball and socket to separate the parts. Reassembly: Reengage the ball and socket to reconnect. Step 4 Disassembly: Remove black cover-ring from upper assembly. Reassembly: Place cover-ring on upper assembly. AUTION VIAFLO pipettes can only be completely disassembled by trained service personnel. www.integra-biosciences.com... -
Page 54: Sterilization
Autoclaving the disassembled components Place the disassembled components (see 6.2) into steam inside an autoclave pouch in the autoclave: Single channel 12.5–1250 µl: Single channel 5000 µl: You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes. INTEGRA Biosciences AG... -
Page 55: Servicing
VIAFLO/VOYAGER – Operating Instructions V08 Maintenance Servicing 6.4.1 Shipping to INTEGRA Biosciences For any service or repairs, please contact your local service technician. ARNING If working with infectious materials, e. g. human pathogens, VIAFLO/ VIAFLO pipettes need to be decontaminated before sending them to service and the declaration on the absence of health hazards must be signed. -
Page 56: Lubrication
VIAFLO/VOYAGER Electronic Pipettes contains a Li-ion battery. Do not modify the battery in any way. Dispose of the pipettes and the batteries separately in accordance with the laws and regulations in your area governing disposal of devices containing Li-ion batteries. INTEGRA Biosciences AG... -
Page 57: Chapter 7 Calibration
It can be adjusted. Precision: Precision describes the repeatability or reproducibility of the measurement. It is also called random error and is therefore an unpredictable error, which cannot be adjusted. Illustration of accuracy and precision. www.integra-biosciences.com... -
Page 58: Materials
• Clean the exterior housing of the pipette and the tip fittings, see 6.1. 7.3.3 Firmware • Check firmware version and perform an update if necessary. Please refer to the sepa- rate update instructions (Operating Instructions VIALINK) when performing an update. INTEGRA Biosciences AG... -
Page 59: Pre-Wetting Of Tips
At the end of a last dispense it is normal to have air bubbles because a blow-out is performed. 6) For a VIAFLO pipette, carry out steps 3-5 for closed and open tip positions (min. and max. tip spacing). www.integra-biosciences.com... -
Page 60: Obtaining The Actual Volume
Leak remedy 1) Change O-rings (see 6.4.2) and lubricate pistons (see 6.4.3) of affected channels. 2) Contact INTEGRA customer support (customersupport@integra-biosciences.com). Obtaining the Actual Volume Perform at least 4 measurements each at 100 %, 50 % and at 10 % of the nominal volume. -
Page 61: Calculation Of Accuracy And Precision
5) to calculate the mean volume -- - Calculation of systematic error (Accuracy) The systematic error s can be calculated using the following equation with s being the selected test volume: or in percent: ---------------------------------- - www.integra-biosciences.com... -
Page 62: Adjusting Electronic Pipettes
The following example is for a 300 µl pipette. Select Toolbox on the Main Menu. Select the Calibration & Service and then the Calibration option. Press OK. Highlight the Pipette Factor. Press OK or Edit to calibrate for Pipette mode. INTEGRA Biosciences AG... - Page 63 Select the Repeat factor in the calibration menu and enter the target and actual (measured) volume. b) Press . c) Then select Pipet factor and enter target and actual volume. d) Press . e) Both factors should be the same now. f) Press Save. www.integra-biosciences.com...
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Page 64: Technical Data
Mains adapter input: 100–240 V, 50/60 Hz Device input: 5.7–6.4 V, 3 W Pipetting channels single, 4, 6, 8, 12 or 16 Pipetting speed 10 steps, adjustable in µl/s Pipetting technology Air displacement User interface Touch Wheel, color display INTEGRA Biosciences AG... -
Page 65: Pipetting Speed
70.5 131.8 314.4 1261 4836 Pipette Size User defined pipetting speed (micro litres per second) Speed 12.5 µl 50 µl 125 µl 300 µl 1250 µl 5000 µl Min. [µl/s] 0.07 Max. [µl/s] 14.01 70.5 131.8 314.4 1261 4836 www.integra-biosciences.com... -
Page 66: Intellectual Property
Repositionable Tips D599,030 Multi-Channel Pipette Multichannel pipettes 7,811,522 Sample Reservoir Kits With Dispos- Reservoirs able Liners D599,031 A Liquid Sample Or Liquid Reagent Reservoirs Reservoir Kit 8,277,757 Pipette Tip Mounting Shaft GripTips 8,501,118 Disposable Pipette Tip GripTips INTEGRA Biosciences AG... -
Page 67: Pipette Specifications
0.75 2500 1.20 0.30 5000 0.60 0.15 4621 0.5–12.5 0.01 1.25 10.00 6.00 6.25 4.00 1.60 12.5 2.00 0.80 4626 2–50 0.05 5.00 2.50 25.0 2.50 0.60 1.50 0.40 4622 5–125 12.5 3.75 1.50 62.5 2.50 0.70 1.60 0.35 www.integra-biosciences.com... - Page 68 2.40 0.50 1250 1.20 0.30 4641 0.5–12.5 0.01 1.25 10.00 6.00 6.25 4.00 1.60 12.5 2.00 0.80 4646 2–50 0.05 5.00 2.50 25.0 2.50 0.60 1.50 0.40 4642 5–125 12.5 3.75 1.50 62.5 2.50 0.70 1.60 0.35 INTEGRA Biosciences AG...
- Page 69 0.05 5.00 2.50 4.5–14.1 25.0 2.50 0.60 1.50 0.40 4722 5–125 12.5 3.75 1.50 4.5–14.1 62.5 2.50 0.70 1.60 0.35 4723 10–300 4.00 1.20 9.0–14.1 2.00 0.60 1.60 0.35 4724 50–1250 6.00 1.10 9.0–14.1 2.40 0.50 1250 1.20 0.30 www.integra-biosciences.com...
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Page 70: Z Correction Factors
1.0033 1.0033 1.0034 1.0034 1.0035 1.0035 1.0036 23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0036 1.0037 24.0 1.0035 1.0036 1.0036 1.0037 1.0037 1.0038 1.0038 24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039 1.0039 Z values in microliters per milligram INTEGRA Biosciences AG... -
Page 71: Chapter 9 Accessories
100-00027-50 1250 μl Replacement kit 24/pack 100-00028-50 5000 μl Replacement kit 10/pack 100-00029-00 O-Ring and Seal Assembly Part No. 12.5 μl O-Ring (black) 300-00158-00 12.5 μl Seal (white) 161922 50 μl Flange (black) 161927 50 μl Seal (white) 161928 www.integra-biosciences.com... - Page 72 Reservoir Base, 10 pack 4304 100 ml 30 reservoirs individually sealed, sterile, 1 base per case 4321 Four sleeves of 50 reservoirs, 200 reservoirs per case, ster- 4322 ile, 1 base per case Reservoir Base, 10 pack 4305 INTEGRA Biosciences AG...
- Page 73 VIAFLO/VOYAGER – Operating Instructions V08 Accessories GripTips for all INTEGRA Pipettes Part No. Purple 5 XYZ racks of 384 tips, sterile, filter, SHORT 6475 SHORT: 12.5 μl Purple Bulk pack, 1 bag of 1000 tips, non-sterile, LONG 4401 LONG: 5 inserts of 384 tips, non-sterile, LONG, GREEN CHOICE 4402 12.5 μl...
- Page 74 VIAFLO/VOYAGER – Operating Instructions V08 Accessories GripTips for all INTEGRA Pipettes Part No. Green: Bulk pack, 1 bag of 1000 tips, non-sterile 4431 300 μl 5 inserts of 96 tips, non-sterile, GREEN CHOICE 4432 5 racks of 96 tips, non-sterile...
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