Using Airyscan - Zeiss LSM 880 with AiryScan FAST Training Manual

Using AiryScan

The AiryScan detection system is designed to increase the resolution and overall image quality compared to traditional confocal
microscopy. The AiryScan is a single detector system – so compared to the confocal – it can only perform line switches for a
maximum of 2 colors. More than 2 tracks will require Frame switching.
1. From the Acquisition toolbar, expand the blue dialog
labeled 'Imaging Setup'.
2. Begin with the longest wavelength fluorophore (Alexa
568 in this case) on Track 1.
Note: By default, ZEN will start with one track (Track 1).
To add additional tracks, click the + button next to the
track list.
3. Click 'Visible Light' to open the laser dialog.
4. Select the laser line you wish to use for this track (561 in
this case for Alexa 568).
5. Set laser power slider (this is a starting value and can be
changed later).
6. Set MBS (Main Beam Splitter) to a filter that matches
the combination of laser lines you will need for ALL
TRACKS.
7. Choose 'Ch A' detector for imaging.
29 – LSM 880 Training Guide