Fluorescence Specifications (Filter-Based) - Biotek Synergy H1 Operator's Manual

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| Appendix A: Specifications

Fluorescence Specifications (Filter-Based)

The Synergy H1 measures fluorescence with filters from the top of 6- to 384-well plates.
The following requirements apply to 96-well plates, with 200 µL/well, at room
temperature. The Detection Limit is calculated as 3 * (Standard Deviation of 8 blank wells)
/ Slope of the concentration curve.
Sodium Fluorescein in phosphate buffered saline (PBS)
DL ≤10 pM, top read
Excitation 485/20 nm, Emission 528/20 nm, 510 nm mirror
Methylumbelliferone (MUB) in carbonate-bicarbonate buffer (CBB)
DL ≤0.16 ng/mL, top read
Excitation 360/40 nm, Emission 460/40 nm, 400 nm mirror
Europium
DL ≤ 250 fM, top read
Excitation 360/40 nm, Emission 620/40 nm, 400 nm mirror
Integration Time
20 to 16,000 µs, Delay 0 to 16,000 µs, Granularity 1-µs steps
Sodium Fluorescein
5 mP standard deviation at 1 nM sodium fluorescein
Excitation 485/20 nm, Emission 528/20 nm, 510 nm mirror
Excitation range 330-700 nm (UV-transparent polarizing filter)
Emission range 400-700 nm
Fluorescence Intensity
Time-Resolved Fluorescence
Fluorescence Polarization
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