Setting Up - Thermo Scientific A2-OK Operating And Maintenance Manual

Thermo Scientific

Setting Up

Section 2
1. For shipping and convenient storage, the gel tray is packaged inside the
unit with the gasketed end gates in
position upon arrival. Lift the casting
tray out of the buffer chamber.
2. To cast gels, place the gel tray(s) into
the external multiple gel caster. The
gasketed sides of the UVT gel tray
form a leak proof seal along the side
of the gel caster.
3. Prepare and pour warm (>60) agarose solution onto UVT gel tray.
Place comb(s) into slots provided on walls of UVT gel tray.
Figure 2-2. Pour Agarose
4. One the gel has solidified, place the UVT gel tray (s) into the gel tray
holder.
5. Level the casting chamber using the thumbscrews on each side of the
unit by slowly turning one thumbscrew at a time and lining up the
bubble in the level with the center circle. Place the gel tray holder in
the buffer chamber, add buffer, remove combs, load samples and run
the gel.
Figure 2-1. Casting Tray
Figure 2-3. Place Combs
Horizontal System 2-1