Using The System - Thermo Scientific A2-OK Operating And Maintenance Manual

Thermo Scientific

Using the System

Section 3
1. Once the gel (s) is completely
solidified, lift the gel tray (s) out of
the external gel caster. Place the gel
tray (s) onto the gel tray holder.
Place the gel tray holder in the
buffer chamber in the running
position. The running position
exposes the open ends of the agarose
to the buffer. Standard agarose
should solidify completely in about
30 minutes.
2. Pour enough compatible running
buffer into the unit to fill the
chamber and completely cover and
submerge the gel. Correct buffer
level is clearly marked on the units
side wall as "FILL LINE". See
Recommended Running Conditions
for approximate buffer volumes
needed for your unit. Too little
buffer may cause the gel(s) to dry
out during the run, while excess
buffer may decrease DNA mobility
and cause band distortion.
3. Carefully remove the comb (or combs) by tapping lightly to loosen,
and slowly lifting straight up out of the gel tray to avoid damage to the
wells.
Figure 3-1. Place Gel Tray
Figure 3-2. Place Gel Tray Holder
Horizontal System 3-1