CEM Razor Installation And Operation Instructions Manual page 7

6. Move the reaction tube containing the rinsed resin to an empty, clean position in the Razor hot block and turn the
numbered valve knob to the CLOSED position.
7. Add cleavage cocktail to the reaction vessel, double that of the volume of the resin bed. For 0.25 mmol cleavages,
the resin mixture may be visible above the hot block. Up to 12 reaction vessels can be loaded into the hot block.
8. Allow the vessel(s) to remain in the Razor for 30 minutes each at 40 - 42 ºC.
If the peptide has multiple Arg residues, increase the cleavage time by 5 min for every additional Arg after the third Arg
residue. Do not exceed 45 min for the total cleavage time.
9. After cleavage has ended, turn the appropriate numbered valve knob to the OPEN position to begin draining the
cleavage solution.
10. Turn the vacuum pump ON to drain any remaining liquid, then turn OFF.
11. Move the Vent valve knob to the OPEN position to release pressure.
12. Unlock the vessel tray by pushing the locking lever up. This will lower the tray for removal.
13. Carefully remove the vessel tray from the Razor cavity and remove the centrifuge tubes containing cleaved peptide.
If moving the tray to a new location, the vessel tray cover (P/N: 170875) can be installed.
14. Replace the used 50 mL centrifuge vessels with clean vessels and move the Vent valve to the CLOSED position.
15. Run a cleaning procedure on each cleavage position used (see Maintenance Procedures).
16. To empty the waste bottle, remove the waste cap and discard the waste then firmly re-secure the cap.
Precipitation and Analysis
1. To precipitate the cleaved peptide, add ice cold ether up to 50 mL (or 8 times the amount of drained liquid). If the
volume exceeds 50 mL, use a second centrifuge tube. The ether should be ice cold to ensure the maximum amount
of peptide precipitates out.
2. Centrifuge the peptide solution for 5 min at 3500 rpm or until a white or clear peptide pellet forms on the bottom of
the tube. Repeat the centrifuge process if necessary to remove any floating particles.
3. Decant the ether, leaving the precipitated peptide in the tube.
4. The peptide can be resuspended in ice cold ether and centrifuged again to ensure all protecting groups are gone.
6
Drain one cleavage position at a time, do not drain multiple positions at once.
Razor™ Parallel Peptide Cleavage System
NOTE
CAUTION
600850 • Revision 2 • July 2017