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WARNING
Handle all chemicals under a fume hood, and wear suitable protective clothing such as safety glasses, chemical
resistant gloves, and a laboratory coat. Dispose of all waste in accordance with all applicable local, state, and
federal health and safety recommendations. For detailed information on the safety requirements for the chemicals
used on the Liberty Blue, refer to the appropriate SDS documents.
This User Guide is designed to highlight the basic chemistry and functions of the Liberty Blue. Read and understand the
Manual, (PN 600291) before operating the Liberty Blue.
For More Information
• Liberty Blue Operation Manual (PN 600291): detailed information about setup and operation of the system.
• CEM website (www.cem.com): list of spare parts, pictures, latest information about CEM's products, references,
application notes, and chemical reagents
• Applications Support: contact CEM at (800) 726-3331 (US/Canada), (704) 821-7015, or by email at
peptide.support@cem.com.
• Technical Support: Contact CEM Service at (800) 726-5551 (US/Canada) or (704) 821-7015
Liberty Blue
Automated Microwave Peptide Synthesizer
User Guide
TM
Copyright CEM Corporation 2018
Part Number 600329
November 11, 2018
Rev. 6
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Table of Contents
Related Manuals for CEM Liberty Blue
Summary of Contents for CEM Liberty Blue
- Page 1 For detailed information on the safety requirements for the chemicals used on the Liberty Blue, refer to the appropriate SDS documents. This User Guide is designed to highlight the basic chemistry and functions of the Liberty Blue. Read and understand the Manual, (PN 600291) before operating the Liberty Blue.
- Page 2 * Notably, His is only stable for one week. Val, Ile, and Leu will begin to precipitate sooner than other amino acids. The reagent dip tube filter(s) will need to be replaced if amino acids begin to crystallize. The Liberty Blue uses stock solutions of all reagents. The table below details the concentrations used on the Liberty Blue and/or CEM’s preferred reagents.
- Page 3 (LL) Mesh Size Only 100-200 mesh (or lower) resin (>75 micron) should be used with the Liberty Blue. The use of higher mesh resins will result in clogging of the reaction vessel frit and damage to the system. Special Case: Hyper Acid Sensitive Resins (e.g. 2-chlorotrityl) Acidic additives (HOBt, HOAt, Oxyma) can lead to premature cleavage of the peptide from the resin (low yields).
- Page 4 Microwave Method Parameters Standard Deprotection Stage Temp ( Power (W) Time (sec) Delta T Standard Coupling Stage Temp ( Power (W) Time (sec) Delta T Microwave Power Specific power settings will vary, depending on scale and the individual microwave. For both the deprotection and coupling methods the set temperature of 90 °C should be reached within 20-30 seconds and maintained for at least 45 seconds and 100 seconds respectively.
- Page 5 Cysteine Cysteine is susceptible to epimerization at elevated temperatures in the presence of bases such as DIEA and NMM. If an uronium activation strategy is being used, Cys should be coupled using the single 50 ° C 10 min coupling cycle with the parameters listed in the table below.
- Page 6 For sequences with multiple Arg(Pbf) residues, cleavage time may need to be extended to ensure complete removal of the Pbf. NOTE The Liberty Blue is not equipped to perform peptide cleavage. All cleavage must be carried out at either room temperature or in the Razor™ parallel cleavage system.
- Page 7 • The resin transfer option is intended for syntheses at 0.1 or 0.25 mmol scale (0.25-1.0 g of resin). • The resin transfer option should only be used with the 30-mL reaction vessel. This option has not been optimized by CEM for use with the 125-mL vessel.
- Page 8 Spare Parts For additional parts visit our website: www.cem.com Filters Part Number Name Description 551005-M Dip Tube Filters Replacement filters for all reagent dip tubes (bag of 30 filters) 551085 Vent Line Filter Replacement filter for the vent line (located before RV1)
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