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5.
Load the vessel tray with 12 clean 50 mL centrifuge tubes (P/N: 330090), and slide into the Razor cavity. Make sure
the tray has been pushed all the way to the back of the cavity. Lock the vessel tray in place by pulling the locking
lever down.
6.
Switch the system power and cavity lights ON using the power switches on the rear of the system.
7.
Switch the system heat ON using the power switch on the left side of the system.
8.
Set and verify the system temperature.
8.1.
Using the up and down arrows on the temperature screen, set the temperature to 40 - 42 ºC. The set
temperature is displayed in green in the lower right corner of the screen.
8.2.
The current temperature is displayed in white in the center of the screen. The system is ready for use when
the current temperature matches the set temperature.
Recommended Cleavage Parameters
In Fmoc synthesis, the removal of the peptide from the solid support is typically accomplished with trifluoroacetic acid (TFA). Because
the side chain protecting groups used in Fmoc synthesis are acid labile, a single step both cleaves the peptide from the resin and
removes the protecting groups. Various scavenger molecules are added to the TFA to prevent the cleaved protecting groups from
reattaching to the peptide. The particular scavengers used depend on the specific peptide sequence. Common scavengers include
water (scavenges t-butyl cations), triisopropyl silane (TIS, scavenges trityl and Pbf cations), ethane dithiol (EDT, scavenges t-butyl
cations, reduces oxidation of Cys/Met side chains), dioxa-1,8-octane-dithiol (DODT, scavenges t-butyl cations, suppresses oxidation
of Cys/Met side chains), phenol (protects Tyr and Trp side chains from oxidation), and thioanisole (aids in removal of Pbf protecting
groups from Arg(Pbf ), suppresses oxidation of Cys/Met side chains).
CEM recommends using a cocktail of
4
TFA/TIS/H
Razor™ Parallel Peptide Cleavage System
600850 • Revision 2 • July 2017
Current Temperature
Set Temperature
Change Temperature
O/DODT
(92.5/2.5/2.5/2.5).
2
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