Integra Evolve 3011 Operating Instructions Manual
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Integra Evolve 3011 Operating Instructions Manual

Manual pipettes
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EVOLVE Manual Pipettes
Operating instructions
900-00010-00_V00

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Summary of Contents for Integra Evolve 3011

  • Page 1 EVOLVE Manual Pipettes Operating instructions 900-00010-00_V00...
  • Page 2 Declaration of conformity INTEGRA Biosciences Corp. – Hudson, NH, USA declares on its own responsibility that the devices Description Models EVOLVE Manual 3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025, Pipettes 3026, 3032, 3034, 3035, 3036 comply with:...

  • Page 3: Table Of Contents

    3.2 Changing the Plunger Spring ............8 Chapter 4 Operation 4.1 Setting the volume................10 4.2 Attaching and removing GripTip pipette tips ........10 4.3 Pipetting ..................11 4.3.1 Aspirating and Dispensing ..........11 4.3.2 Reverse mode..............12 4.3.3 Proper pipetting techniques ..........13 4.4 Troubleshooting................14 www.integra-biosciences.com...
  • Page 4 5.2 Single channel lower end disassembly ..........15 5.2.1 Disassembly................15 5.2.2 Reassembly ................18 5.3 Sterilization ..................20 5.3.1 Autoclaving the disassembled components......20 5.4 Servicing..................21 5.4.1 Shipping to INTEGRA Biosciences........21 5.4.2 Changing O-rings of tip fittings..........21 5.4.3 Lubrication ................22 5.5 Calibration ..................23 5.5.1 Materials ................23 5.5.2 Definitions ................23 5.5.3 Test conditions and environment ........24...

  • Page 5: Chapter 1 Introduction

    This symbol identifies important notes regarding the correct operation of the device and labor-saving features. Intended use EVOLVE Manual Pipettes are intended to be used in a laboratory for aspirating and dispensing liquids in the volume range of 0.2–5000 µl using GripTip pipette tips. www.integra-biosciences.com...

  • Page 6: Safety Notes

    Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after- sales service member.

  • Page 7: Description Of The Device

    1 Plunger Cap 2 Plunger, to pipette 3 Tip Ejector 4 Finger Hook 5 Adjustment Dials, to set volume 6 Volume Window The color of the volume indicator label matches GripTip box insert 7 Ejector Sleeve 8 Tip Fitting www.integra-biosciences.com...

  • Page 8: Chapter 3 Installation

    3) Put the two teeth of the Multipurpose Tool (d) vertically into the slots of the black opening inside at the bottom of the pipette‘s body (c). Rotate counterclockwise until plunger spring assembly (e) is released. INTEGRA Biosciences AG...
  • Page 9 (e) clockwise into the upper part of the pipette using the Multipurpose Tool. 5) Re-attach the Piston assembly/Piston. Screw clockwise to attach the Cylinder Assembly (a) to the body of the pipette. AUTION Validate or calibrate your pipette after reassembly. www.integra-biosciences.com...

  • Page 10: Chapter 4 Operation

    Discard your used tips: If liquid is in the tips, empty them by pressing down the Plunger (2) to the second stop (see section 4.3). Tips are easily removed by pressing the Tip Ejector (3). INTEGRA Biosciences AG...

  • Page 11: Pipetting

    5) To dispense the liquid, push the Plunger (2) down to the first stop. 6) If there is any residual liquid in tip, a blowout should be performed by pressing the Plunger to the second stop. 7) Remove the GripTips from vessel and release Plunger. www.integra-biosciences.com...

  • Page 12: Reverse Mode

    3) To dispense, press the Plunger (2) slowly down to the first stop and wait a few seconds to dispense the liquid. 4) Remove the GripTip(s) from the target vessel. 5) To expel the excess liquid press the Plunger to the second stop. INTEGRA Biosciences AG...

  • Page 13: Proper Pipetting Techniques

    4.3.3 Proper pipetting techniques INTEGRA Biosciences recommends the following techniques for enhancing pipetting results. These techniques are consistent with ISO standard 8655-2. • It is best to immerse the GripTips just below the surface of the liquid (2–3 mm) to allow the desired volume to be aspirated.

  • Page 14: Troubleshooting

    • Pre-wet tips up to 3 times. tips. from that of air inside the tips. • Liquid of low viscosity and • Increase dispensing speed. high vapor pressure. • Touch off was not performed. • Perform a touch-off. INTEGRA Biosciences AG...

  • Page 15: Chapter 5 Maintenance

    1) Unscrew counterclockwise remove Cylinder Assembly (a) from the pipette. 2) Slide the Cylinder Assembly (a) down from the pipette body. This exposes the Piston Assembly (b, models 2–200 µl) or Piston with mounted Cup Seal (m, model 1000 µl). www.integra-biosciences.com...
  • Page 16 Set these components aside or place them in an autoclave pouch. 100 µl 200 µl Models 2–1000 µl: 4) Separate the Piston (h) from the upper part of the pipette. The Piston is held in place by a small magnet. INTEGRA Biosciences AG...
  • Page 17 Remove the Piston (h) with mounted Cup Seal (m). 2–1000 µl 5000 µl Models of all sizes: 6) Remove the Ejector Spring (j). 7) Set all components of the lower part aside or place them in an autoclave pouch. www.integra-biosciences.com...

  • Page 18: Reassembly

    Piston Assemblies (models 2–200 µl) and Pistons with Cup Seal (1000 µl, 5000 µl): c: Seal Retainer d: Seal Retainer Spring e: Lower Seal Retainer f: O-Ring g: Seal h: Piston m: Cup Seal 2, 10, 20 µl 100, 200 µl 1000 µl 5000 µl INTEGRA Biosciences AG...
  • Page 19 2–1000 µl 5000 µl Models of all sizes: 10) Screw clockwise to attach the Cylinder Assembly (a) to the body of the pipette. Perform a leak test (see “5.5.5 Leak test” on page 25) and validate pipetting volumes after reassembly. www.integra-biosciences.com...

  • Page 20: Sterilization

    Place the disassembled components (see 5.2.1) into steam autoclave in an autoclave pouch: Single channel 2–1000 µl: Single channel 5000 µl: You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes. AUTION Service is required after autoclaving the EVOLVE Manual Pipettes! INTEGRA Biosciences AG...

  • Page 21: Servicing

    EVOLVE Manual Pipettes – Operating instructions V00 Maintenance Servicing 5.4.1 Shipping to INTEGRA Biosciences For any service or repairs, please contact your local service technician. ARNING If working with infectious materials, e. g. human pathogens, the EVOLVE Manual Pipettes need to be decontaminated before sending them to service and the declaration on the absence of health hazards must be signed.

  • Page 22: Lubrication

    O-ring (b) and slide it over the white seal (c). Single channel 1000 µl and 5000 µl models: Use the Super-O-Lube (#100-00135-50) to lightly lubricate the outer ring of the Cup Seals (d, e). Do not lubricate the bottom of Cup Seals. INTEGRA Biosciences AG...

  • Page 23: Calibration

    Additionally, it equilibrates humidity of the air space inside tip and the pipette. Touch off: Touching the pipette tip against the surface liquid or side of well-plate to release any sample liquids that might be on the end of the pipette tip. www.integra-biosciences.com...

  • Page 24: Test Conditions And Environment

    5) Dispense: Keep the Plunger pressed to the first stop until all liquid is expelled. At the end of the third cycle push the Plunger to the second stop, remove the tip from the liquid and then release the Plunger to perform a two-step blowout. INTEGRA Biosciences AG...

  • Page 25: Leak Test

    4) Droplets are forming when holding the pipette in the air for 20 seconds, even if a pre- wet was performed. 5) The liquid levels are not equal on all channels after aspiration. If the pipette is leaking, change O-rings (see 5.4.2) and lubricate pistons (see 5.4.3) or contact your service technician. www.integra-biosciences.com...

  • Page 26: Obtaining The Actual Volume

    Maintenance 5.5.6 Obtaining the actual volume INTEGRA certifies the pipettes at 10%, 50% and 100% of the nominal value. For each volume 5 measurements are taken from all channels. General 1) Always use new, unused GripTips, also when changing the test volume (e.g. from 200 µl to 20 µl).

  • Page 27: Calculation Of Accuracy And Precision

    Add together the volumes i delivered, divide the sum by n (e.g. n = 5) to calculate the mean volume (in millilitres or micro litres) delivered at the test temperature, which is the Actual Volume:   -- - www.integra-biosciences.com...

  • Page 28: Adjusting Evolve Manual Pipettes

    The industry standard is to test and present specifications using neat transfers. This is aspirating and dispensing the same volume. In case a EVOLVE Manual Pipette has not met the calibration specifications it needs to be adjusted. This can be corrected with the Multipurpose Tool. INTEGRA Biosciences AG...

  • Page 29: Equipment Disposal

    Validate the new volume set by repeating the gravimetric testing. Equipment disposal The EVOLVE Manual Pipettes must not be disposed of with unsorted municipal waste. Dispose of the EVOLVE Manual Pipettes in accordance with the laws and regulations in your area. www.integra-biosciences.com...

  • Page 30: Technical Data

    Precision Increments Volume (µl) (±%) (≤%) (µl) (µl) 3011 0.2–2 0.001 3012 1–10 0.01 3013 2–20 0.01 0.35 3015 10–100 0.35 3016 20–200 0.18 3018 100–1000 1000 3019 500–5000 2500 5000 0.18 Precision = Coefficient of Variation. INTEGRA Biosciences AG...
  • Page 31 1–10 0.01 3024 5–50 0.05 3025 10–100 3.75 3026 20–200 3032 1–10 0.01 3034 5–50 0.05 3035 10–100 3.75 3036 20–200 The multichannel pipettes’ specifications represent the performance of all channels of the pipettes. Precision = Coefficient of Variation. www.integra-biosciences.com...

  • Page 32: Z Correction Factors

    1.0033 1.0033 1.0034 1.0034 1.0035 1.0035 1.0036 23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0036 1.0037 24.0 1.0035 1.0036 1.0036 1.0037 1.0037 1.0038 1.0038 24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039 1.0039 Z values in microliters per milligram INTEGRA Biosciences AG...

  • Page 33: Accessories And Consumables

    Parker Super-O-Lube, Silicone based, 50 g / 2 oz, for single 100-00135-50 channel 1250 µl and 5000 µl pipettes Grease Grease Nye Fluorocarbon Gel 807, 5 g / 0.2 oz, for single 100-00136-50 channel 12.5 µl, 125 µl and 300 µl pipettes www.integra-biosciences.com...
  • Page 34 EVOLVE Manual Pipettes – Operating instructions V00 Accessories and Consumables GripTips for all INTEGRA Manual and Electronic Pipettes Part No. Purple Bulk pack, 1 bag of 1000 tips, non-sterile, LONG 4401 LONG: 5 inserts of 384 tips, non-sterile, LONG, GREEN CHOICE 4402 2 μl,...
  • Page 35 EVOLVE Manual Pipettes – Operating instructions V00 Accessories and Consumables GripTips for all INTEGRA Manual and Electronic Pipettes Part No. Orange: Bulk pack, 1 bag of 250 tips, non-sterile 4451 5 ml 5 racks of 48 Tips, non-sterile 4453 5 racks of 48 Tips, sterile...

  • Page 36: Imprint

    © 2016 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.

This manual is also suitable for:

Evolve 3012Evolve 3013Evolve 3015Evolve 3016Evolve 3018Evolve 3019 ... Show all

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