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Summary of Contents for Integra EVOLVE 3011
- Page 1 EVOLVE Manual Pipettes Operating Instructions 900-00010-00_V06...
- Page 2 Declaration of Conformity INTEGRA Biosciences Corp. – Hudson, NH, USA declares on its own responsibility that the devices Description Models EVOLVE Manual 3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025, Pipettes 3026, 3028, 3032, 3034, 3035, 3036, 3038, 3042, 3044, 3045...
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Page 3: Table Of Contents
4.2 Single channel lower end disassembly ..........13 4.2.1 Disassembly................13 4.2.2 Reassembly ................16 4.3 Sterilization ..................18 4.3.1 Autoclaving the disassembled components......18 4.4 Servicing..................19 4.4.1 Shipping to INTEGRA Biosciences........19 4.4.2 Changing O-rings of tip fittings..........19 4.4.3 Lubrication ................20 4.5 Calibration ..................21 4.5.1 Materials ................21 4.5.2 Definitions ................21 4.5.3 Test conditions and environment ........22... - Page 4 5.1 Environmental conditions ...............28 5.2 Pipette specifications..............29 5.3 Z correction factors.................31 Chapter 6 Accessories and Consumables 6.1 Accessories ..................32 6.2 Consumables .................32 6.3 GRIPTIPS..................33 6.3.1 GRIPTIPS for handheld pipettes.........33 6.3.2 Package options ..............33 6.3.3 GRIPTIP properties.............33 Imprint ....................34 INTEGRA Biosciences AG...
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Page 5: Chapter 1 Introduction
IVD setting is under the sole responsibility of the user. EVOLVE manual pipettes are intended to be used in a laboratory for aspirating and dispensing liquids in the volume range of 0.2–5000 µl using GRIPTIP pipette tips. www.integra-biosciences.com... -
Page 6: Safety Notes
(fluor-rubber), NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive vapors could also damage metallic parts inside the device. • Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after-sales service member. • Parts may be replaced with original INTEGRA Biosciences parts only. -
Page 7: Description Of The Device
Overview of the EVOLVE manual pipettes 1 Plunger cap 2 Plunger 3 Tip ejector 4 Finger hook 5 Volume adjustment dials 6 Volume window The color of the volume indicator label matches GRIPTIP rack insert 7 Ejector sleeve 8 Tip fitting www.integra-biosciences.com... -
Page 8: Chapter 3 Operation
Discard your used tips: If liquid is in the tips, empty them by pressing down the plunger (2) to the second stop (see section 3.3). Tips are easily removed by pressing the tip ejector (3). INTEGRA Biosciences AG... -
Page 9: Pipetting
5) To dispense the liquid, push the plunger down to the first stop. 6) If there is any residual liquid in tip, a blowout should be performed by pressing the plunger to the second stop. 7) Remove the GRIPTIPS from vessel and release plunger. www.integra-biosciences.com... -
Page 10: Reverse Mode
3) To dispense, press the plunger slowly down to the first stop and wait a few seconds to dispense the liquid. 4) Remove the GRIPTIPS from the target vessel. 5) To expel the excess liquid, press the plunger to the second stop. INTEGRA Biosciences AG... -
Page 11: Proper Pipetting Techniques
3.3.3 Proper pipetting techniques INTEGRA Biosciences recommends the following techniques for enhancing pipetting results. These techniques are consistent with ISO standard 8655-2. • It is best to immerse the GRIPTIPS just below the surface of the liquid (2–3 mm) to allow the desired volume to be aspirated. -
Page 12: Troubleshooting
• Pre-wet tips up to 3 times. tips. from that of air inside the tips. • Liquid of low viscosity and • Increase dispensing speed. high vapor pressure. • Touch off was not performed. • Perform a touch-off. INTEGRA Biosciences AG... -
Page 13: Chapter 4 Maintenance
3.1. 2) Unscrew the cylinder assembly (a) clockwise and gently slide it down from the pipette body. This exposes the piston assembly (b, models 2– 100 µl) or piston with mounted cup seal (models 200–1000 µl). www.integra-biosciences.com... - Page 14 It is held in place by a magnet. Slide finger nail under the smaller diameter of the cup seal (m) and push it off the Piston. Set these components aside or place them in an 1000 µl 200 µl autoclave pouch. INTEGRA Biosciences AG...
- Page 15 (k) in hand unscrew the upper part. Remove the piston (h) with mounted cup seal. Remove the ejector spring (j). Models of all sizes: 5) Set all components of the lower part aside or place them in an autoclave pouch. www.integra-biosciences.com...
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Page 16: Reassembly
The extended lip faces the upper part of the pipette. The sleeve should be resting on the ejector spring. Models 200 µl, 1000 µl: 4) Attach cup seal (m) to the piston (h). Insert the piston into the greased cylinder assembly. INTEGRA Biosciences AG... - Page 17 7) Position the piston at the small magnet to connect the piston to the upper part of the pipette. 8) Screw the cylinder assembly (a) counterclockwise to attach it to the body of the pipette. Perform a leak test (see “4.5.5 Leak test” on page 23) and validate pipetting volumes after reassembly. www.integra-biosciences.com...
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Page 18: Sterilization
Autoclaving the disassembled components Place the disassembled components (see 4.2.1) into steam autoclave in an autoclave pouch. You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes. AUTION Service is required after autoclaving the EVOLVE Manual Pipettes! INTEGRA Biosciences AG... -
Page 19: Servicing
EVOLVE manual pipettes – Operating instructions V06 Maintenance Servicing 4.4.1 Shipping to INTEGRA Biosciences For any service or repairs, please contact your local service technician. ARNING If working with infectious materials, e. g. human pathogens, the EVOLVE manual pipettes need to be decontaminated before sending to service and the declaration on the absence of health hazards must be signed. -
Page 20: Lubrication
Cup Seal (m). Do not lubricate the bottom of Cup Seals. Single channel 5000 µl model: Use the Kluberalfa Lube (#500-00182-00) to lightly lubricate the outer ring of the Cup Seal (m) and O-Ring (o). Do not lubricate the bottom of Cup Seals. INTEGRA Biosciences AG... -
Page 21: Calibration
Additionally, it equilibrates humidity of the air space inside tip and the pipette. Touch off: Touching the pipette tip against the surface liquid or side of well-plate to release any sample liquids that might be on the end of the pipette tip. www.integra-biosciences.com... -
Page 22: Test Conditions And Environment
5) Dispense: Keep the plunger pressed to the first stop until all liquid is expelled. At the end of the third cycle push the plunger to the second stop, remove the tip from the liquid and then release the plunger to perform a two-step blowout. INTEGRA Biosciences AG... -
Page 23: Leak Test
4) Droplets are forming when holding the pipette in the air for 20 seconds, even if a pre- wet was performed. 5) The liquid levels are not equal on all channels after aspiration. If the pipette is leaking, change O-rings (see 4.4.2) and lubricate pistons (see 4.4.3) or contact your service technician. www.integra-biosciences.com... -
Page 24: Obtaining The Actual Volume
Maintenance 4.5.6 Obtaining the actual volume INTEGRA certifies the pipettes at 10%, 50% and 100% of the nominal value. For each volume 5 measurements are taken from all channels. General 1) Always use new, unused GRIPTIPS, also when changing the test volume (e.g. from 200 µl to 20 µl). -
Page 25: Calculation Of Accuracy And Precision
Add together the volumes i delivered, divide the sum by n (e.g. n = 5) to calculate the mean volume (in milliliters or micro liters) delivered at the test temperature, which is the Actual Volume: -- - www.integra-biosciences.com... -
Page 26: Adjusting Evolve Manual Pipettes
The industry standard is to test and present specifications using neat transfers. This is aspirating and dispensing the same volume. In case an EVOLVE Manual Pipette has not met the calibration specifications it needs to be adjusted. This can be corrected with the Multipurpose Tool. INTEGRA Biosciences AG... -
Page 27: Equipment Disposal
In certain regions and countries, e.g. in EU member states, the distributor is obliged to take back this product free of charge at the end of life. Please contact your local distributor for more details. www.integra-biosciences.com... -
Page 28: Technical Data
EVOLVE manual pipettes – Operating instructions V06 Technical data Technical data Environmental conditions Operation Temperature range 5–40°C Humidity range Max. rel. humidity 80% for temperatures up to 31°C, decreasing linearly to 50% rel. humidity at 40°C. Altitude range <2000 m INTEGRA Biosciences AG... -
Page 29: Pipette Specifications
EVOLVE manual pipettes – Operating instructions V06 Technical data Pipette specifications The specifications apply only to neat transfers when the instrument is used together with INTEGRA GRIPTIPS and represent the performance of all channels on a multichannel pipette. EVOLVE manual pipettes Manufacturer... - Page 30 Accuracy Precision increments volume (µl) (±%) (≤%) (µl) (µl) 3022 1–10 0.01 12.0 3024 5–50 0.05 3025 10–100 3026 20–200 3028 100–1250 1250 3032 1–10 0.01 12.0 3034 5–50 0.05 3035 10–100 3036 20–200 3038 100–1250 1250 INTEGRA Biosciences AG...
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Page 31: Z Correction Factors
1.0034 23.0 1.0033 1.0033 1.0034 1.0034 1.0035 1.0035 1.0036 23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0036 1.0037 24.0 1.0035 1.0036 1.0036 1.0037 1.0037 1.0038 1.0038 24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039 1.0039 Z values in microliters per milligram www.integra-biosciences.com... -
Page 32: Accessories And Consumables
For single channel pipettes 2, 10, 20, 100, 200 and 1000 µl only 3230 Consumables O-rings Part No. for 200/300 µl tip fittings, pack of 24 100-00027-50 for 1000/1250 µl tip fittings, pack of 24 100-00028-50 for 5000 µl tip fittings, pack of 10 100-00029-00 INTEGRA Biosciences AG... -
Page 33: Griptips
Nye fluorocarbon gel 807, 5 g, for single channel pipettes 2 µl – 10 µl 100-00136-50 GRIPTIPS INTEGRA offers a wide range of GRIPTIPS in the volume ranges from 12.5 µl – 5000 µl. Visit the GRIPTIP Selector Guide (www.integra-biosciences.com/griptips) to find the correct GRIPTIPS and set a filter by available volumes, packaging and properties. -
Page 34: Imprint
© 2023 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
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