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2
– Installation
2.3
System Requirements
Dionex CarboPac Columns are designed to run on Dionex Ion Chromatographs equipped with
electrochemical detectors. We recommend the use of ferrules and fittings rated with a pressure of
>5000 psi. The use of precut tubing, complete with high pressure fitting and ferrules, is
recommended for easier and more secure installation.
For 1-mm as well as for 0.4mm Dionex CarboPac PA200 Columns, 0.0025" i.d. PEEK (Item #
071870) tubing is required for all the connections after the EGC 400 MSA cartridge. Make sure
that extra care is taken to minimize dead volume.
2.3.1
Installation of Disposable Electrode into a Dionex ICS-5000 ED Cell, pH-Ag/AgCl Reference
Electrode or PdH Reference Electrode
The 1 mil (0.001") thick Teflon gaskets (Item # 072161) are required; otherwise, the disposable
electrode product warranty is void. Always wear gloves when handling electrodes. Never touch
the electrode surface. To install a disposable working electrode and reference electrode (pH-
Ag/AgCl or PdH) refer to Product Manual for Disposable Electrodes Doc. No. 065040, ICS-5000
Ion Chromatography System Manual Doc. No. 065342 and User's Compendium for
Electrochemical Detection Doc. No. 065340.
In addition, the quadruple waveform must be used for carbohydrate analysis otherwise the product
warranty is void. The standard, recommended carbohydrate waveform is shown in Table 1.
Table 1
!
Do not polish a new gold electrode prior to use. NEVER POLISH the disposable gold
electrodes.
NOTE
2.3.2
System Void Volume
When using Dionex CarboPac PA200, 1 mm and 0.4 mm columns, it is particularly important to
minimize system void volume. For best performance, all of the tubing installed between the
injection valve and detector should be 0.0025" i.d. PEEK tubing (Item # 071870) for both 1mm
and 0.4 mm. Minimize the lengths of all connecting tubing and remove all unnecessary switching
valves and couplers.
Thermo Scientific
065734-01
Carbohydrate Quadruple Waveform
Time (sec)
0.00
0.20
0.40
0.41
0.42
0.43
0.44
0.50
Product Manual for CarboPac PA200 Columns
For Research Use Only. Not for use in diagnostic procedures.
Potential (V)
vs. Ag/AgCl
+0.1
+0.1
+0.1
–2.0
–2.0
+0.6
–0.1
–0.1
Integration
Begin
End
Page 10 of 37
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