Biotek Synergy HT Operator's Manual page 213

Code
Memory allocation failed
1900
This error indicates that the process failed while saving or moving data. If this occurs, try turning
the instrument off, waiting for 30 seconds, and then turning the instrument back on.
Probable Causes:
• The memory is corrupt. Replace the processor PCB.
• If the error persists, contact BioTek TAC.
1C00 A/D calibration STBY line never went low
A/D calibration STBY line went low but never transitioned to a high
1C01
This error indicates a failure with one of the PCBs when trying to initialize the A/D, or the cable
to the defective PCB has lost continuity.
This error can indicate one of the following A/D circuits:
• Fluorescence analog PCB.
• Absorbance analog PCB.
Probable Causes:
• Fluorescence analog PCB or connection between the analog PCB and motor power supply
PCB.
• Absorbance analog PCB or the cable between the analog PCB and motor power supply
PCB.
Band-pass overlap in filter set
1F01 – 1F02
This error indicates an open hole or overlapping filter set within the optical path of the excitation
and emission filter wheels designated by the protocol.
Equation used for overlapping:
If EM wavelength is > EX
if (EX wavelength + ½ bandpass) >= (EM wavelength – ½ bandpass) then fail
else if EX wavelength is > EM
if (EM wavelength + ½ bandpass) >= (EX wavelength – ½ bandpass) then fail
Note: Only 2 filter sets can be sent when developing software (not Gen5 or KC4). 1F01 is for the
first set; 1F02 is for the second set.
2100 Invalid parameter value selected
This error can occur only during computer control, indicating that an invalid assay configuration
was sent to the instrument.
2200 PMT signal too low
This error indicates that during the 750V test, the PMT signal was less than 1. If this is true, then
increase the gain to 1250V and test for PMT signal > 1. If PMT signal is still < 1, the PMT is not
connected or is defective.
Probable Causes:
• The PMT is not connected
• The PMT analog PCB, PMT base, or the PMT is defective
PMT test signal too high at the 750-volt bias
2201
See 750V measurement on self-test. Measured value > 3000 to fail.
This error indicates that the PMT dark current is higher than the specified limit.
Probable Causes:
• The PMT has been saturated due to ambient light leakage.
• The PMT has been damaged.
• The PMT base is defective, or the coaxial cable from the base has lost the ground connection
for the shield.
Synergy HT Operator's Manual
Description and Probable Causes:
191
Error Codes |