Gilson P10M User Manual page 12

USER'S GUIDE
 Changing the tip-ejector
1) Keep the tip-ejector button depressed and grip the top of
the tip-ejector with the other hand.
2) Gently rotate the tip-ejector counter clockwise and separate
its connector from the operating rod.
3) Pull the tip-ejector away from the body of the pipette.
4) Clean or autoclave the tip-ejector and refit or replace it by
reversing the procedure.
 Changing the tip-holder (lower part)
After removing the tip-ejector, you may remove the lower part
of the tip-holder, which is more likely to become contaminated
or damaged than the upper part. Removal of the lower part
is shown below; for the upper part see "Removing the Piston"
(special precautions are necessary).
1) Gently rotate the lower part of the tip holder counterclock-
wise to unscrew it from the upper part.
2) Separate the parts and remove the O-ring (see "Changing
O-ring")
3) Clean and if required autoclave the lower part of the tip-
holder (autoclaving: 20 min. at 121°C and 0.1MPa)
4) If required, lubricate the piston (see "How to Lubricate the
Piston") and fit a new O-ring.
5) Screw the two parts together, making sure that the two
parts are fully tightened, by hand.
6) Refit the tip-ejector.

After autoclaving the tip-holder and tip-ejector may change color, however this has no impact on the performance.
 Changing the seal and/or O-ring (see Chapter 20)
The O-ring is positioned on the piston; it should not be autoclaved, if worn
or damaged in any way, it must be replaced.
To access the O-ring, remove the tip-ejector and unscrew the lower part
of the tip-holder. You should now be able to remove the O-ring from the
piston. Sometimes, the O-ring may be found in the recess at the top-end of
the lower part of the tip-holder. If required, lubricate the piston (see "How
to Lubricate the Piston") then fit a new O-ring by sliding it onto the piston.
Reassemble the pipette. Depending on the pipette model the dimensions
of the O-ring will vary (refer to Chapter 20).
 Servicing the piston
You may remove the piston-assembly to clean, lubricate, or change the piston.
PIPETMAN M
Tip-holder
(upper)
Tip-holder
(lower)
O-ring
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EN EN
 Removing the piston
1) Remove the tip-ejector and (optionally) the lower
part of the tip-holder. If you remove the lower
part, take care to remove the O-ring as described
in "Changing the O-ring".
2) Unscrew the connecting nut (turn by hand, coun-
terclockwise).
3) Gently remove the connecting nut and upper tip
holder.
4) Pull off the piston assembly from the body of the
pipette – separate the parts (see below).
5) Clean and autoclave (if required) the piston and
holder, together with any other parts that may need
to be treated in the same way (see Chapter 15).

In the case of P1200M, the piston holder, piston and
guide are a unit. Don't try to separate them!
6) Lubricate the piston, see below.
7) Reassemble the piston and guide; then carefully insert the assembly into the body of the pipette.
The guide should hold the piston assembly inside the body of the pipette. You can hear a "click"
Piston
when the piston is back in place.

Take care not to touch the piston, and that it is dust-free. If needed, clean the assembly with a dust-free cloth.
Tip-holder (lower)
8) Reassemble the upper part of the tip-holder and
the connecting nut, and then refit to the body
of the pipette by rotating the connecting nut
clockwise until it is finger tight. Fit the O-ring (for
P10M, the seal) and reassemble the lower part of
the tip-holder. Refit the tip-ejector.
The seals for P10M are fragile and can be used only
once. So, after unscrewing the lower part of the tip-
holder you must fit a new seal.
 How to lubricate the piston (except P10M)
Use only Gilson lubricant (ref: F2070902, as supplied).
Squeeze a small quantity from the tube onto a clean, lintless cloth. Use the cloth to transfer the lubricant
to the piston. Ensure that the piston is evenly lubricated and that you wipe away any excess – remember
only a fine film of lubricant is required (over the entire piston).
PIPETMAN M
The specifications of the pipette
must be checked after changing
any part!
Don't pull on the piston to remove
the assembly!
Tip-holder (upper) Piston Piston holder
Guide
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USER'S GUIDE
Guide Connecting nut
Connecting nut