System Startup - Nikon A1+ User Manual

System Startup

*See the next page for labelled images of all devices
1. Prior brightfield LED (only if required). Switched on via Prior Optiscan II box
2. Lasers-Turn the key on the front of the laser bed clockwise to the horizontal position
(405/458/488/514/561 excitation lines)
3. The 640nm far-red laser (only if required) is turned on via the black rocker switch.
The LED light will stay orange for 30secs or so and then turns green. Once this
happens turn the key on the box to the horizontal position.
4. A1 controller (button on left side of tower)
5. XY stage controller (on shelf)
6. MCL Piezo controller (on shelf, only if required)
7. PC
Additional devices required for Live Cell Imaging
• Incubation chamber heater (chamber doors must be on while heater is in use)
• CO2 controllers (see labelled diagram)
CO2 flow valve on wall (see labelled diagram)
•
8. Log in to Windows using the "User" account
9. If using the 640nm laser launch its control
software from the desktop icon
10. Select the Power mW button and type 30 into the text
box
11. Press the "On" button
12. You can minimise the Fiber laser window but don't close it
13. Launch Nis-Elements from the desktop icon. If you have
will have a username to log in to Elements
3
been trained then you