Nis-Elements Software Layouts; Selecting A Confocal Channel Setup - Nikon A1+ User Manual

Firefly confocal
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Nis-Elements software layouts

1. Nis-Elements has many toolbars which need to be changed depending on the
experiment you are carrying out. The Layout tabs at the bottom left side of the
screen allow you to change the software layout which essentially changes the
toolbars visible too you.
2. When you start Elements the "Confocal" layout will be selected by default. This
contains all of the toolbars required to capture confocal images. The padlock icon
denotes that the "Confocal" layout cannot be altered.
3.
The "User" layout can be selected if you want to customise the layout of the
software. If you change this layout profile, any changes will only be applied within
your Nis-Elements user account.

Selecting a confocal channel setup

1. Some default channel presets are available from the OC Panel under the "Confocal-
Channel-Settings" heading. Selecting one will initialise the relevant lasers/detectors.
Ask facility staff if the defaults are not suitable for your application.
2.
The number of detectors and lasers that are active will
display in the A1Plus Compact GUI in the right hand margin
on the screen
7

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