Image Saturation - Nikon A1+ User Manual

Image Saturation

1. As well as the image being too dark, it can also be too bright. The confocal
acquires 12bit images. This means that any pixel in the image can have an
intensity value between 0 (black)-4095 (white). The aim is to set the scan
parameters so that the pixels are just below saturation level (4095).
2. In the right hand margin in the software below the
Compact GUI is the LUTs tab. Select the tab and click
on the Pixel Saturation Indicator button. When active this
will colour any pixels in the image that have reached the
4095 maximum intensity value. The colour given to over
saturated pixels can be selected through the down arrow
next to the button. An image window must be open in
order for the Pixel Saturation Indicator button to appear.
3. Images showing the saturation indicator turned on and
off. Saturated pixels in this case have been coloured
yellow.
4. Continue to scan using the Channel Setup option, switch between the detectors
optimising each image so that it is just below saturation intensity level (no or
minimal pixels at 4095 intensity).
5.
If you stop scanning this automatically turns off the Channel Setup option and
pressing Scan again will activate all lasers/detectors.
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