Appendix; Saving Images; Export Images In Tiff Format - Nikon A1+ User Manual

Saving Images

Select the image window to save then select File>Save As from the top menu.
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Enter a file name and select ND2 as the file format.

Export images in tiff format

1. Open the Image to be exported then select File>Import/Export>ND export to tiff...
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2. Under Output select Browse to choose where the tiffs will be stored and use the File
Prefix box to name the file
3. Index order allows you to choose whether separate tiff images are created for each
plane of a z stack or whether a multi-page tiff is created (single tiff stack image
containing all z planes)
4. Tiff Compatibility Options determines at what bit depth the tiff files will be stored.
Keep bit depth- If you want to keep the raw intensity data for quantification then use
this option. This will save the images as a 16bit tiff but the pixel intensities will not
be changed and remain within the 12bit range 0-4095 as they were when the image
was acquired.
Scale 12bit to 16bit- A 16bit tiff is created and the intensities will be within the 16bit
0-65520 range. You cannot use this image for intensity quantification
Scale 12bit to 8bit- An 8bit tiff is created and the intensities are scaled within the
8bit range, 0-255. This can be useful if you want a copy of the image that can be
viewed in any program. Again the raw intensity data is lost with this option.
5. Channels determines how the different channels are handled in the exported tiff
Mono image for each channel- A tiff image is created for each channel and is
greyscale
Merge channels to RGB image- A single tiff image is created which will show a
colour merge of the channels. This still contains the raw data if you select to keep
bit depth as well

Appendix

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