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Start system
• Turn on the power by the power socket (on the floor under the table)
• Switch the mercury lamp on and then ignite it.
o If not needed don't turn the mercury lamp on
o The unit on the shelf closest to the door
• Turn on all devices with a button on the shelf and the table.
o Camera and laser box are automatically enabled
• Next turn on the device with a key: Laser control panel
• Start the computer and start the program Metamorph.
o Select suited user account.
Live Cell imaging:
• When imaging at 37
• The heating device to maintain your cells uses water.
o fill reservoir with demineralized water up to level of CO2 tube
• Allow the system to warm up for 15-30 minutes
• Settings for the heating unit are paper on the wall, choose your settings
o General settings for cells on a coverslip:
o top heater 40.5 ° C
o bath heater 38.5 ° C
o stage heater 38.5 ° C
o lens heater 37 ° C
• Ensure that the cells are grown on a glass coverslip
• Place the slide in the metal ring, tighten firmly but not too hard because it will break the
glass
• Add 1-2 ml of the medium from the appropriate well. There is a convex surface
• Move with a paper towel around the edge of the coverslip from below with a little pressure,
if no medium leaks from the ring your seal is good.
• Wipe clean the underside of the slide with ethanol to prevent contamination of the
objective and microscope
Martijn de Gruiter
o
C:
Manual: TIRF Microscope
Erasmus OIC
Ee-1454
3
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