Hemoglobin Denaturant Storage Conditions; Sample Mixing (Vortex Mixing Vs. Pipet Mixing); Vortex Mix - Abbott AEROSET Troubleshooting Manual

Hemoglobin Denaturant Storage Conditions

Sample Mixing (Vortex Mixing vs. Pipet Mixing)

®
AEROSET System Troubleshooting Guide
94816-107—November 2004
PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
The recommended storage temperature for the Hemoglobin
Denaturant is 2 to 8°C. A study was performed to evaluate the
effect of room temperature storage of Hemoglobin
Denaturant. The study used the same lot of Hemoglobin
Denaturant, with one bottle stored at 4°C and another stored
at room temperature (22°C). One normal and one high sample
were used for the evaluation. Results are listed in the following
table.
HbA
g/dL
Level 1, Rep 1 0.75
Level 1, Rep 2 0.75
Level 2, Rep 1 1.62
Level 2, Rep 2 1.61
There is little to no apparent difference between the HbA
result for the room temperature (22°C) and 4°C denaturant
solutions.
A study was performed to evaluate effects of vortex mixing and
pipet mixing on sample results. Procedures to perform this
study are included below.

Vortex Mix

1. 400 µL denaturant was added to plastic tubes, using the
same tip for three tubes.
2. Blood sample was pipetted by rinsing the tip once with
blood.
3. The pipet tip was wiped clean on the outside, with
precautions taken to not wick any sample.
4. Blood was dispensed into denaturant and the tip was
rinsed two times.
5. The treated sample was vortexed at medium speed
approximately five seconds.
Room Temp
THb HbA HbA
1c 1c
g/dL %
15.2 4.9
15.3 4.9
14.1 10.7
14.9 10.8
Assays/Reagents
2 to 8°C
THb HbA
1c 1c
g/dL g/dL %
0.73 14.1 5.2
0.73 14.7 5.0
1.52 13.8 11.0
1.50 13.9 10.8
1c
137