Factor; Enzyme Factor Calculation - Abbott AEROSET Troubleshooting Manual

Factor

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AEROSET System Troubleshooting Guide
94816-107—November 2004
PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
The Factor option only displays if Factor is selected as the
calibration mode. This method allows the operator to enter
the factor used to calculate the result as a concentration.
The Factor math model can be used for any reaction that is
linear and stable across all reagent lots. The calibration factor
is defined at a fixed value and only the reagent blank is
performed for calibration. No calibrators are required. It is
ideal for use in measuring enzyme activity in a sample,
because the enzyme reaction occurs at a predictable and steady
rate that can be determined for the chromophore,
wavelengths, and volume parameters used.

Enzyme Factor Calculation

The following formula is used to calculate enzyme factors.

TotalaVolumeaxa1000

SampleaVolume
Where:
Total Reagent 1 volume + Reagent 1 water volume +
Volume Reagent 2 volume + Reagent 2 water volume +
Sample volume
(Extinction factor) established by measuring
varying concentrations of the chromophore
(NADH, PNP, etc.) on the system. The slope of
the linear regression of the measured
absorbance versus the concentration equals the
extinction factor. The factor is specific for the
AEROSET and c 8000
characteristics of these systems.
Enzyme Entered in the Factor field on the Calibration
Factor page of the Assay Configuration screen.
a
1a
  
a = EnzymeaFactor
ε
  
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Systems, and optical
Assays/Reagents
37