Assay Characteristics; Instrument Troubleshooting - Abbott AEROSET Troubleshooting Manual

Flowcharts
Abbott Assay—Basic Assay Troubleshooting, Continued

Assay Characteristics

Instrument Troubleshooting

248
PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
If multiple assays are affected, look for similar characteristics
such as:
• Same line
• Assays using R1 only vs. assays using R1 and R2
• Similar wavelengths
• Same calibrators
• Same sample and reagent volumes
• Rate vs. end-point reaction
If the issue affects one assay:
• Verify assay parameters with the assay-specific package
insert
• Was a different bottle of reagent, calibrator, or control
used, and was it handled correctly?
• Was a different lot of calibrator or reagent used?
• Are there any non-Abbott assays on the original line? If so,
were carryover studies performed between those assays
and the affected assay?
• Move the assay to an alternate line. If the issue is not
resolved, proceed to step 2 of Instrument
Troubleshooting.
1. Is the issue on both lines? If yes, troubleshoot common
items, including:
• Lamp
• Cuvette washer
• Water
• Bulk solution use
• Maintenance
• Probe/mixer wash flow
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AEROSET System Troubleshooting Guide
94816-107—November 2004