Chemical Reactions For Clinical Chemistry Assays - Abbott AEROSET Troubleshooting Manual

Assays/Reagents

Chemical Reactions for Clinical Chemistry Assays

Assay
Name
ACP
α-Napthol + Diazotized 2-amino-5-chlorotoluene
AlbG
(BCG)
AlbP
(BCP)
p-Nitrophenylphosphate + H
AlkP
L-Alanine + α-Ketoglutarate
ALT
®
* MULTIGENT assay
42
PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
Reaction
α-Naphthylphosphate
Albumin + BCG
BCG = Bromcresol green
Human Albumin + BCP
BCP = Bromcresol purple
AlkP
O
2
Mg
Pyruvate + NADH
LD = Lactate dehydrogenase
NADH = Nicotinamide adenine dinucleotide
ACP
α-Napthol + PO -3
4
Dye complex
Colored complex
Colored complex
p-Nitrophenol + phosphate
+2 +2
, Zn
ALT
Pyruvate + L-glutamate
LD
+
L-lactate + NAD
Tips
Use fresh,
nonhemolyzed,
nonicteric, and
nonlipemic serum
samples. All
specimens need to be
stabilized with 50 µL
Liquid Stabilizer for
every 1 mL of serum.
The acidified serum
should be analyzed
immediately, or
stored at 2 to 8°C, or
frozen. Calibration is
stable for
approximately five
days and performed
using a water blank.
Sample Interference
Indices may assist
with determination of
sample integrity.
Results are running
high, due to
non-specific protein
binding. Recommend
AlbP if more
specificity needed.
Human albumin
specific.
AlkP reagent contains
AMP buffer.
Do not use plasma
samples collected
using ammonium
heparin.
®
AEROSET System Troubleshooting Guide
94816-107—November 2004