Ettan Daltsix Electrophoresis System Function And Description; System Function And Description; Electrophoresis Unit; Gel Caster - GE HEALTHCARE Ettan DALTsix User Manual

Electrophoresis system

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Ettan DALTsix Electrophoresis

System function and description

1.1

In 2-D electrophoresis, proteins are separated according to isoelectric point by isoelectric focusing, most reliably

on Immobiline™ DryStrip immobilized pH gradient (IPG) gel strips using the IPGphor™ or Multiphor™ II IEF

Systems. The second-dimension electrophoresis separates the proteins on the basis of their molecular mass

using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The Ettan™ DALTsix

Electrophoresis System is designed to handle large second-dimension gels in a simple, efficient, and

reproducible manner.

1.1.1

The Ettan DALTsix Electrophoresis Unit accommodates up to six 25.5 × 20.5 cm slab gels, either 1 mm or 1.5 mm

thick, in a common tank under identical conditions. A sample, focused in an IPG strip, is placed on the cathodic

(upper) surface of a slab gel and sealed in place with agarose. Up to six gel cassettes are inserted into the

electrophoresis unit and any unused slots are filled with blank cassette inserts. The Upper Buffer Chamber/Buffer

Seal (UBC) is pushed down over the cassettes, holding the gel cassettes in slots flanked by a double rubber

gasket.

Power is supplied to the unit by an external source such as an EPS 601 Power Supply. The unit is capable of

handling 600 V, 400 mA, or 100 W.

A pump mounted under the lower buffer chamber circulates the buffer, pumping it up into the chamber on the

right-hand side, between the cassettes, down the left side, and over the internal heat exchanger before returning

to the pump. The pump starts when it is plugged in. For temperature control, the heat exchanger located in the

bottom of the unit must be connected to a MultiTemp™ III or similar circulating water bath.

1.1.2

The Ettan DALTsix Gel Caster holds up to six 1-mm or 1.5-mm gel cassettes, with separator sheets, for casting

homogenous or gradient gels. When desired, fewer gels can be cast at one time by using blank cassette inserts

to occupy any unneeded volume. The removable faceplate and separator sheets simplify loading and unloading

the casting unit. The groove in the back of the caster provides the channel through which the gel solution is

Fig 1-1. Exploded view of Ettan DALTsix Electrophoresis Unit.

Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC