Unloading The Gel Caster - GE HEALTHCARE Ettan DALTsix User Manual

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Unloading the gel caster

WARNING! When using hazardous chemicals, take all suitable protective measures, such as wearing
protective glasses and gloves resistant to the chemicals used. Follow local regulations and instructions for
safe operation and maintenance of the system.
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Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC
Make sure the caster is either near a sink or on a tray so that any liquid leaking out can be contained.
Remove the front of the gel caster by loosening and removing the black-knobbed screws and spring clamps.
Carefully unload the cassettes from the unit by pulling forward on the separator sheets.
Rinse the top surface of each gel with distilled water to remove the n-butanol and any unpolymerized
acrylamide. Remove the separator sheet if still attached and rinse the glass cassettes with water to remove
any acrylamide adhering to the glass plates.
Examine the gels for polymerization defects and discard any unsatisfactory gels.
Store the acceptable gels in an airtight container at 4 ºC with a small amount of gel storage solution to keep
the gels from drying out.
Rinse the gel caster and all tubing with mild detergent then rinse thoroughly with deionized water. Clean the
separator and spacer sheets with a mild detergent and rinse with deionized water.
Unloading the gel caster 6
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