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7 Using Ettan DALTsix for electrophoresis
7.3 Recommended running conditions
7.2.1
There are two alternatives for removing the completed gels from the electrophoresis unit:
Alternative A:
1
2
3
4
5
6
Alternative B:
1
2
3
4
7.3
Run conditions (Day run):
Applicable to 1 mm thick 12% PAA gel and Laemmlie Buffer
system. Set the MultiTemp temperature to 25ºC.
Step
1
2
1
For 1.5 mm gels, increase the current by 50%.
2
Continue the electrophoresis until the bromophenol blue
reaches the end of the gel.
24
Unloading the gels and cleaning the unit
Carefully pull the UBC upward using the handles at each end.
As the UBC is pulled upward make sure that the cassettes remain in the anode assembly.
Completely remove the UBC, then remove the gel cassettes from the anode assembly.
Open the gel cassettes using a Wonder Wedge (80-6127-88) to separate the plates on the side opposite the
hinge.
For lab cast gels, run an edge of the Wonder Wedge down each side of the cassette along the spacer and
carefully lift the gel out of the cassette. For pre-cast gels, lift the gel out by grasping the GelBond backing.
Rinse all the components with distilled or deionized water. Flush the pump with distilled or deionized water
by filling the unit and turning the pump on.
Insert the cassette removal tool carefully between the cassette and the buffer seal, with the folded tip facing
the cassette, until the tip is beneath the bottom edge of the cassette. Verify that the tool is caught on the
bottom edge of the cassette then lift it slowly with the tool.
Remove all the cassettes from the unit then lift the UBC off the anode assembly.
Open the gel cassettes using a Wonder Wedge (80-6127-88) to separate the plates on the side opposite the
hinge.For lab cast gels, run an edge of the Wonder Wedge down each side of the cassette along the spacer
and carefully lift the gel out of the cassette. For pre-cast gels, lift the gel out by grasping the GelBond
backing.
Rinse all the components with distilled or deionized water. Flush the pump with distilled or deionized water
by filling the unit and turning the pump on.
Recommended running conditions
mA/
Voltage
gel
(V)
1
80
10
1
500
38
Run Conditions (Overnight):
Set the MultiTemp temperature to 12ºC.
W/
Time
Step
gel
(hours:
mins)
1
1:0
1
13
4:30-
2
6:00
2
1
For 1.5 mm gels, increase the current by 50%.
2
Continue the electrophoresis until the bromophenol blue
reaches the end of the gel.
Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC
mA/
Voltage
W/
gel
(V)
gel
1
80
1
10
150
2
12
1
Time
(hours:
mins)
1:0
14:00-
16:00
2
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