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Laboratory Equipment
AKTAprime plus
GE HEALTHCARE AKTAprime plus Manuals
Manuals and User Guides for GE HEALTHCARE AKTAprime plus. We have
2
GE HEALTHCARE AKTAprime plus manuals available for free PDF download: Operating Instructions Manual, Quick Reference Instructions
GE HEALTHCARE AKTAprime plus Operating Instructions Manual (66 pages)
compact liquid chromatography system designed for one-step purification of proteins at laboratory scale
Brand:
GE HEALTHCARE
| Category:
Laboratory Equipment
| Size: 1.47 MB
Table of Contents
Table of Contents
2
1 Introduction
4
Important User Information
5
Regulatory Information
6
Instrument
9
Monitoring and Evaluation
13
User Documentation
13
2 Safety Instructions
15
Safety Precautions
15
Labels
23
Emergency Procedures
25
Recycling Information
26
Declaration of Hazardous Substances (Dohs)
27
3 Installation
29
Site Requirements
29
Transport
29
Unpacking
30
Connections
30
Spare Parts and Accessories
31
4 Operation
32
Operation Overview
32
Starting the Instrument
32
Preparations before Start
33
Performing a Run
38
Procedures after a Run
40
5 Maintenance
42
General
42
User Maintenance Schedule
43
Cleaning
45
Component Maintenance
46
Disassembly and Assembly of Components and Consumables
46
Calibration
47
Storage
48
6 Troubleshooting
49
UV Curve Problems
49
Conductivity Curve Problems
50
Ph Curve Problems
52
Pressure Curve Problems
55
7 Reference Information
56
Specifications
56
Chemical Resistance
57
System Recommendations
60
Health and Safety Declaration Form
61
Ordering Information
63
Appendix A Connection Diagram - Liquid Flow Path
64
Appendix B Tubing
65
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GE HEALTHCARE AKTAprime plus Quick Reference Instructions (40 pages)
Brand:
GE HEALTHCARE
| Category:
Medical Equipment
| Size: 1.39 MB
Table of Contents
Table of Contents
1
System Preparation
2
Buffer Exchange on Hitrap Desalting
4
Buffer Exchange on Hiprep 26/10 Desalting
6
Histidine-Tagged Protein Purification, Step Elution
8
Histidine-Tagged Protein Purification, Gradient Elution
10
IMAC Purification - any Metal
12
On-Column Refolding
14
GST-Tagged Protein Purification
16
Strep(II)-Tagged Protein Purification
18
MBP-Tagged Protein Purification
20
Mab Purification, Step Elution
22
Mab Purification, Gradient Elution
24
Igm Purification
26
Albumin Removal
28
Removal of Trypsin-Like Serine Proteases
30
Anion Exchange
32
Cation Exchange
34
Method Templates Value Table
36
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