Preparing The Ettan Daltsix Gel Caster - GE HEALTHCARE Ettan DALTsix User Manual

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Preparing the Ettan DALTsix Gel Caster

Set up the gel caster near a sink, in a tray, or on a drainboard so that any liquid that overflows, spills, or drains
out of the unit during pouring or disassembly can be easily contained.
The Ettan DALTsix Gel Caster accommodates up to six 1-mm or 1.5-mm gel cassettes with separator sheets
(0.5 mm) between them. If you are not planning to cast a full set of gels, use the blank cassette inserts (purchased
separately) with separator sheets between them to occupy the extra space.
Gel labels, for easy indexing of gels and samples, can be placed in the cassettes at any time during the assembly
of the caster.
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2
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4
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Fig 3-1. Gel caster with parts labelled.
Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC
Check that the caster is level. Remove the faceplate and lay the caster on its back. If casting gels by the
displacement method, remove the triangular wedge in the V-shaped base.
Fill the gel caster starting with a separator sheet against the back wall. The separator sheets make it easier
to remove the cassettes from the unit after polymerization. Fill the caster by alternating cassettes with
separator sheets. The rubber hinge should be on the left side of the caster, with the matching ends of the
cassette down. End with a separator sheet then use the thicker filler sheets (1.0 mm) to bring the level of the
stack of cassettes and spacers even with the edge of the caster.
Remove the gray foam seal from the groove in the faceplate and lubricate it with a light coating of GelSeal
compound to help ensure a liquid-tight seal. Place the gasket back in the groove on the faceplate. Avoid
stretching the gasket by seating it from the ends first, working toward the middle.
Turn two black knobbed screws into the two threaded holes across the bottom until they are well engaged
(two to three full turns). Carefully place the faceplate onto the caster with the bottom slots resting on their
respective screws. Clamp both sides of the faceplate with six spring clips and tighten the screws. Be sure the
sealing gasket is compressed evenly by the faceplate and forms a tight seal with the caster. Do not
overtighten the screws.
If casting using the filling channel in the back plate of the caster, be sure to plug the barbed fitting in the
faceplate. If casting using the displacement method, attach the tubing from the peristaltic pump to the
barbed fitting.
filler sheets
filling channel
separating sheets
gel cassette
cassette hinge
spring clamp (6)
faceplate and
sealing gasket
filler port
and cap
clamp screw
Preparing the Ettan DALTsix Gel Caster 3
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