Recipes - GE HEALTHCARE Ettan DALTsix User Manual

8

Recipes

Acrylamide stock (30.8 %T)
Acrylamide (MW 71.08)
Bis (N,N'-methylenebisacrylamide, MW 154.17)
Distilled or deionized water
May need filtration. Weigh acrylamide and bis under a hood; avoid contact with dust. Filter and store at 4 ºC.
1.5 M Tris-Cl, pH 8.8
Tris (MW 121.14)
6 M HCl to pH 8.8
Distilled or deionized water
Adjust to pH 8.8 and store at 4 ºC.
10% (w/v) SDS
Sodium dodecylsulfate (MW 288.38)
Distilled or deionized water
Store at room temperature.
10% (w/v) Ammonium persulfate
Ammonium persulfate (MW 71.08)
Distilled or deionized water
Prepare fresh.
10% (v/v) TEMED
TEMED (MW 116.2)
Distilled or deionized water
Prepare fresh.
Displacing solution
(0.375 M Tris-Cl, pH 8.8, 30% (v/v) glycerol, bromophenol blue, 200 ml)
Tris-Cl (1.5 M, pH 8.8)
Glycerol
Bromophenol blue
Distilled or deionized water
Should be made fresh; stored solution may support microbial growth.
Water-saturated butanol
n, i, or t-butanol
Distilled or deionized water
Combine in a bottle and shake. Use the top phase to overlay gels. Store at room temperature indefinitely.
Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC
final conc. amount
30% 900 g
0.8% 24 g
to 3 000 ml
final conc. amount
1.5 M 545 g
about 150 ml
to 3 000 ml
final conc. amount
10% 10 g
up to 100 ml
final conc. amount
10% 2 g
up to 20 ml
final conc. amount
10% 0.5 ml
4.5 ml
amount
50 ml
60 ml
2 mg
90 ml
amount
50 ml
10 ml

Recipes 8

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