GE HEALTHCARE Ettan DALTsix User Manual
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GE HEALTHCARE Ettan DALTsix User Manual

Electrophoresis system
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GE Healthcare
Ettan DALTsix
Electrophoresis System
User Manual

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Summary of Contents for GE HEALTHCARE Ettan DALTsix

  • Page 1 GE Healthcare Ettan DALTsix Electrophoresis System User Manual...
  • Page 3 (12 psig). Do not connect it to a water tap. Connect only to coolant sources with regulated pressure. – connected to other products recommended or described in this manual, – used in the same state as it was delivered from GE Healthcare except for alterations described in this manual. Recycling...
  • Page 4 Hersteller nicht spezifiziert wurde. ment est utilisé d'une façon non indiquée par le constructeur. • Nur Originalzusatzteile und Ersatzteile, die vom GE Healthcare geneh- • Seuls les accessoires et piéces detachées approuvés ou fournis par migt oder geliefert wurden, dürfen zum Betrieb, zur Erhaltung und zur GE Healthcare sont recommandés pour l'utilisation, l'entretien et rép-...

  • Page 5: Table Of Contents

    Contents Contents Ettan DALTsix Electrophoresis System function and description 1.1 Ettan DALTsix Electrophoresis System function and description ................7 1.1.1 Electrophoresis Unit ................................7 1.1.2 Gel Caster .....................................7 1.1.3 Gel Casting Cassettes ..............................8 1.1.4 Gradient Maker ..................................8 Specifications 2.1 Ettan DALTsix Electrophoresis Unit ............................9 Preparing the Ettan DALTsix Gel Caster ..................11...
  • Page 6 Contents Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 7: Ettan Daltsix Electrophoresis System Function And Description

    Electrophoresis Unit The Ettan DALTsix Electrophoresis Unit accommodates up to six 25.5 × 20.5 cm slab gels, either 1 mm or 1.5 mm thick, in a common tank under identical conditions. A sample, focused in an IPG strip, is placed on the cathodic (upper) surface of a slab gel and sealed in place with agarose.

  • Page 8: Gel Casting Cassettes

    1.1.3 Gel Casting Cassettes The Ettan DALTsix System uses the same cassettes as the Ettan DALTtwelve Electrophoresis System (see Fig 1-3). The gel casting cassettes are preassembled. Two glass plates are joined along one edge by a hinge strip of silicone rubber, and the vinyl side spacers (1.0 mm or 1.5 mm thick) are glued in place.

  • Page 9: Specifications

    Safety standards This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the following harmonized standards: • EN 61010-1 • IEC 61010-1 • CAN/CSA-C22.2 No. 61010-1 • UL61010-1 Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 10 Cassette dimensions (w × h × d) 27.6 × 21.7 × 0.75 cm Slab gel dimensions (w × h × d) 25.5 × 20.5 × 0.15 cm Ettan DALTsix Gradient Maker Volume 1000 ml Weight 0.73 kg Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 11: Preparing The Ettan Daltsix Gel Caster

    The Ettan DALTsix Gel Caster accommodates up to six 1-mm or 1.5-mm gel cassettes with separator sheets (0.5 mm) between them. If you are not planning to cast a full set of gels, use the blank cassette inserts (purchased separately) with separator sheets between them to occupy the extra space.
  • Page 12 3 Preparing the Ettan DALTsix Gel Caster Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 13: Casting Homogeneous Gels

    Do not leave the water-saturated n-butanol on the gels overnight as long term exposure of acrylic to butanol will damage the caster. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 14 4 Casting homogeneous gels Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 15: Casting Gradient Gels

    Preparation for gradient gel casting Successful gradient gel casting requires planning, timing, and practice. A full cast with the Ettan DALTsix Gel Caster requires 420–550 ml of acrylamide stock. Polymerization begins as soon as TEMED and APS are added to the acrylamide stock.

  • Page 16: Pouring Gel Solutions For Gradient Gels

    10 When the gels have reached the desired height and before air is introduced, stop the peristaltic pump. It is important that no air bubbles disturb the gradient. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 17 Fig 5-2. Open and closed positions of slide valve. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 18 5 Casting gradient gels 5.3 Pouring gel solutions for gradient gels Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 19: Unloading The Gel Caster

    Rinse the gel caster and all tubing with mild detergent then rinse thoroughly with deionized water. Clean the separator and spacer sheets with a mild detergent and rinse with deionized water. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 20 6 Unloading the gel caster Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 21: Using Ettan Daltsix For Electrophoresis

    IPG strip is on the left. The gel face of the strip should not touch the opposite glass plate. (See Fig 7-1). Fig 7-1. IPG strip are loaded onto cassette and slid into place. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 22: Preparing The Electrophoresis Unit

    Preparing the electrophoresis unit The Ettan DALTsix electrophoresis unit requires a total volume of about 5.0 l of electrophoresis buffer to fill both the upper and lower chambers. For lab cast Laemmli gels, the lower chamber requires approximately 4.3 l of 1×...
  • Page 23 (Fig 7-3 D). Place the safety lid on the unit and begin electrophoresis. MAX fill line MIN fill line LBC start fill line with cassettes Fig 7-3. Filling the buffer chambers. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 24: Unloading The Gels And Cleaning The Unit

    For 1.5 mm gels, increase the current by 50%. Continue the electrophoresis until the bromophenol blue Continue the electrophoresis until the bromophenol blue reaches the end of the gel. reaches the end of the gel. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 25: Recipes

    50 ml Distilled or deionized water 10 ml Combine in a bottle and shake. Use the top phase to overlay gels. Store at room temperature indefinitely. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 26: Volume Required For (Ml)

    600 ml volume required for (ml) final %T 12.5% Acrylamide stock 1.5 M Tris-Cl, pH 8.8 Water 10% SDS 6.00 6.00 6.00 10% APS 6.00 6.00 6.00 10% TEMED 1.03 0.83 0.69 Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 27: Volume Required For (Ml) Final %T 8% 10% 12% 14

    %T Acrylamide stock 1.5 M Tris-Cl, pH 8.8 Water 10% SDS 2.75 2.75 2.75 2.75 2.75 Glycerol 10% APS 2.75 2.75 2.75 2.75 2.75 10% TEMED 0.59 0.47 0.39 0.34 0.29 Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 28: Acrylamide Stock

    (ml) final %T Acrylamide stock 1.5 M Tris-Cl, pH 8.8 Water 10% SDS 2.75 2.75 2.75 2.75 2.75 Glycerol 10% APS 10% TEMED 0.13 0.11 0.10 0.09 0.08 Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 29: Troubleshooting

    Check recipes, gel concentrations, and buffer dilutions. (For example, do not use Tris·HCl in place of Tris base for the electrophoresis buffer.) Discard older acrylamide solutions and use only reagents of highest quality. Only use freshly deionized urea of highest quality. Adjust power, current, or voltage. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 30 Prepare equilibration solution according to instructions. weight proteins Poor transfer of protein from IPG strip to second Use low power for sample entry phase. Extend entry phase dimension gel. if necessary. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 31 8.8. Protein spots are at both extremes The molecular weight range of the sample requires an acrylamide concentration gradient to resolve the full but not in center range of proteins. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 32 9 Troubleshooting Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 33: Care And Maintenance

    This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment. Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 34 10 Care and maintenance 10.3 Recycling Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...

  • Page 35: Customer Service Information

    11.1 Technical service and repair GE Healthcare offers complete technical support for all our products. If you have any questions about how to use this product, or would like to arrange to repair it, please contact your local GE Healthcare representative for more information.
  • Page 36 17-0615-01 Companion Products EPS 601 Power Supply 6 - 600 V, 1- 400 mA, 1-100 W 18-1130-02 MultiTemp III Thermostatic Circulator, 230 V 18-1102-78 MultiTemp III Thermostatic Circulator, 115 V 18-1102-77 Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 37 Customer service information 11 Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 38 11 Customer service information 11.1 Technical service and repair Ettan DALTsix Electrophoresis System User Manual 80-6492-49 Edition AC...
  • Page 40 Ettan, Immobiline, IPGphor, Multiphor and MultiTemp are trademarks of GE Healthcare companies. GE, imagination at work and GE monogram are trademarks of General Electric Company. All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies GE Healthcare Bio-Sciences AB them.

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