Flow Charts - GE ÄKTA start Manual

%B
100
50
3 CV
IEX Step 1 ml/5 ml HiTrap - Ion Exchange Chromatography
The bound proteins are eluted in a single step using single
elution buffer.
%B
100
50
5 CV
IEX Gradient 1 ml/5 ml HiTrap - Ion Exchange
Chromatography
The bound proteins are eluted using two buffers with linear
increase in the concentration of buffer B over a specified
time, followed by a step with 100% B concentration.
%B
100
50
5 CV 5 CV
GF 16/60 HiPrep - Gel Filtration Chromatography
• The proteins are eluted in a single step using single
elution buffer.
• The column needs to be pre-equilibrated before the
start of the run.
%B
100
50
0.2 CV
10
Fractionation/collection
1.2 CV
Fractionation/
collection
5 CV 5 CV 5 CV
Fractionation/
collection
20 CV 5 CV 5 CV
Fractionation/collection
1.2 CV

Flow charts

The active flow path is depicted in Green color.
A. Flow path details for flow of 100% buffer A
through Outlet valve to waste
B. Flow path details for flow of 100% buffer B
through Outlet valve to waste
C. Flow path details during gradient
operation at specified B concentration
D. Flow path details, Outlet valve collection
Note: Fraction collector is disabled.
ÄKTA start System Cue Card, 29-0240-42 AC