Phase Contrast Device - kruss MBL2000 Series Operating Manual

7. Phase contrast device

Depending on the object lens in use, the scale length of
10 units corresponds to:
Object lens
Object lens 10x = 1000 um
Object lens 40x =
Object lens 100x =
Only one pointer or micrometer eyepiece should be used
for the binocular microscope as otherwise there maybe a
double image of the scale.
7. Phase contrast device
The phase contrast device can be adapted to the fol-
lowing microscopes:
MML1500, MBL2000 and MBL2000-T
MML 2032 The phase contrast device MML 2032 consists of a
positive phase contrast lens with a phase lag ring (20x),
a centring ring diaphragm and a centring telescope,
dark field and bright field.
MML 2031 The phase contrast device MML 2031 consists of a
positive phase contrast lens with a phase lag ring (40x),
a centring ring diaphragm and a centring telescope,
dark field and bright field.
MML 2030 The phase contrast device MML2030 consists of a
positive phase contrast lens and a centring diaphragm
for 10x, 40x and 100x magnifications and a centring
telescope.
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4x = 2500 um
250 um
100 um
Technical data and specifications
The system works according to the positive phase
contrast method. The individual phase contrast
elements have the following data:
PH-1 Achromatic phase contrast lens
10x NA 0.25 with ring diaphragm in a centring mount.
PH-2 Achromatic phase contrast lens
40x NA 0.65 with ring diaphragm in a centring mount.
PH-3 Achromatic phase contrast lens 100x NA 1.25 for oil
immersion with diaphragm ring in a centring mount.
Centring telescope with an adjustable eyepiece.
Functional principle When examining colourless, transparent samples in
the bright field, the contrast is often too low. In order
to avoid colouring or to examining living samples,
the phase contrast method can be used.
Coloured samples can be examined because the
different components have different densities. In case
of transparent samples, details in the bright field are
not easy to examine as all components have the same
density.
7. Phase contrast device
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