Olympus BX53 Instructions Manual
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Olympus BX53 Instructions Manual

Olympus BX53 Instructions Manual

System microscope with led light source
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Instructions
BX53
System Microscope
With LED light source
To ensure safety, obtain optimum performance and to familiarize yourself fully with the use
of this product, we recommend that you study this manual thoroughly before operating
this product, and always keep this manual reachable when operating this product.
Optical Microscope and Accessory
For details of products included in the configuration of this microscope, see page 7.

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  • Page 1 Instructions BX53 System Microscope With LED light source To ensure safety, obtain optimum performance and to familiarize yourself fully with the use of this product, we recommend that you study this manual thoroughly before operating this product, and always keep this manual reachable when operating this product.
  • Page 2 In accordance with European Directive on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local our distributor in EU for return and/or collection systems available in your country.

  • Page 3: Table Of Contents

    Contents Introduction ............................... 1 Safety precautions ............................2 1 Nomenclature of units ........................7 2 Transmitted light brightfield observation procedures ..........8 2-1 Basic operation (Up to observation of specimen) .............10 2-2 Microscope adjustment..........................11 Adjusting the field diaphragm (FS) ..........................11 Adjusting the interpupillary distance ........................
  • Page 4 3-3 Stage ..................................22 Placing the specimen ................................22 Using the auxiliary specimen holder ........................23 Adjusting the tension of X-axis / Y-axis knob ....................24 Rotating the stage ..................................25 3-4 Observation tube ............................26 Adjusting the tilt ...................................26 Using the eye shades ................................27 Selecting between the eyepiece light path and the camera light path ................27 3-5 Condenser ................................
  • Page 5 7 Troubleshooting ............................ 42 7-1 Optical systems ..............................42 7-2 Electrical systems ............................45 7-3 Coarse/fine focusing ........................... 45 7-4 Observation tube ............................. 46 7-5 Stage ..................................46 8 Specifications ............................47 9 Optical performance list <<UIS2 series>> ................50 10 Assembly ..............................

  • Page 7: Introduction

    Read all the instruction manuals supplied with the units you purchased. The following instruction manuals are prepared for the units to be used with this product. Instruction manuals Main contents BX53 system microscope Transmitted light brightfield observation (this instruction manual) Motorized reflected fluorescence...

  • Page 8: Safety Precautions

    Safety precautions If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the product may also be damaged. Always use the product according to this instruction manual. The following symbols are used in this instruction manual.
  • Page 9 BX53 CAUTION - Carrying the microscope - Hold the handheld portion of the microscope and the front portion of the base. When carrying the microscope, hold the handheld portion on the rear of the arm and the front portion of the base. Holding the slippery...
  • Page 10 CAUTION - Prevention of electric shock - Keep the power cord and cables well away from the lamp housing. If the power cord and cables contact a hot area of the lamp housing, they could melt and cause electric shock. Never insert any tools or metal fragments in the air vents of the product.

  • Page 11: Handling Precautions

    BX53 CAUTION - Safety symbols - The following symbols are placed on this product. Study the meaning of the symbols and always use the product in the safest possible manner. Symbol Meaning Indicates a non-specific general hazard. Follow the description given after this symbol or in the instruction manual.

  • Page 12: Intended Use

    Intended use This product has been designed to be used to observe magnified images of specimens in various routine work and research applications. This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological information at hospitals or laboratories.

  • Page 13: Nomenclature Of Units

    BX53 Nomenclature of units The following figure shows the main units only. For other units combinable with the microscope, contact us or refer to the latest catalogs. For units marked “*”, refer to the instruction manuals provided separately. Observation tube ·...

  • Page 14: Transmitted Light Brightfield Observation Procedures

    Transmitted light brightfield observation procedures Turn ON the main switch. Adjust the brightness. Select the light path. (Trinocular tube only) Place a specimen on the stage. Engage the 10X objective in the light path. Bring the specimen into focus. Perform the centering of the field diaphragm. Adjust the interpupillary distance.
  • Page 15 BX53 Interpupillary distance adjustment scale (page 13) Light path selection lever (page 27) Diopter adjustment ring(page 13) Condenser centering knob (page 11) Condenser height adjustment knob (page 11) Revolving nosepiece (page 10) Allen screwdriver Specimen holder (page 22) Y-axis knob (page 10)

  • Page 16: Basic Operation (Up To Observation Of Specimen)

    2-1 Basic operation (Up to observation of specimen) This section describes the basic operations of the microscope up to the observation of a specimen. For operating procedures of each operation unit in detail, see the corresponding page. Turn the main switch of the microscope frame to (ON). When the power is ON, there is one beep and the pilot indicator a turns ON.

  • Page 17: Microscope Adjustment

    BX53 2-2 Microscope adjustment Adjusting the field diaphragm (FS) Using the field diaphragm (FS) Rotate the field diaphragm ring a to adjust the field diaphragm. Field diaphragm ring index position Field Open to Close to diaphragm maximum minimum Centering the field diaphragm (FS) ·...
  • Page 18 Field diaphragm image Rotate the condenser height adjustment knob to bring the field diaphragm image into focus. (Picture A) Field of view of the eyepiece Rotate the centering knobs (2 positions) to adjust the field diaphragm image to come to the center of the field of view. (Picture B) Rotate the field diaphragm ring in the arrow direction t o o p e n t h e f i e l d d i a p h ra g m g ra d u a l l y u n t i l t h e f i e l d d i a p h ra g m i m a g e f i t s i n t h e f i e l d o f v i e w.

  • Page 19: Adjusting The Interpupillary Distance

    BX53 Adjusting the interpupillary distance The adjustment of the interpupillary distance is to adjust the distance between two eyepieces to fit to the distance between your two eyes. By doing so, you can see the single microscope image so that the fatigue of your eyes during observation can be reduced.
  • Page 20 Change to the 40X or higher objective again, and rotate the coarse and fine focusing knobs to bring the specimen into focus. Change to the 10X objective, and while looking through the right and left eyepieces, check that the specimen is in focus. If the specimen is not in focus, repeat from again.

  • Page 21: Adjusting The Aperture Diaphragm (As)

    BX53 Adjusting the aperture diaphragm (AS) Using the aperture diaphragm (AS) When the aperture diaphragm ring is equipped with the scale a Rotate the aperture diaphragm ring to set the index b to 70% of the numerical aperture of the objective While looking at the observed image, rotate the aperture diaphragm ring and finely adjust it to obtain the desired contrast.

  • Page 22: Operation Procedures Of Each Part

    Operation procedures of each part 3-1 Transmitted light illumination adjustment part Adjusting the brightness Rotate the brightness control knob a of the microscope frame clockwise to increase the brightness of the lamp. Using the PRESET switch PRESET is a function to set to the PRESET (pre-stored) brightness automatically regardless of the position of the brightness control knob.

  • Page 23: Using The Lim And Lim Set Switches

    BX53 Using the LIM and LIM set switches This function is available when the LED light source is com- bined with the motorized or coded nosepiece. What is LIM function? LIM stands for "Light Intensity Manager". For each objective whose arbitrary brightness was stored in advance, this function sets the stored brightness automatically when the objective is selected.

  • Page 24: Using The Filter

    Procedure to replay the brightness Press the LIM switch a to set the "Replay" mode. (The LIM indicator b is ON.) When the objective is selected, the stored brightness is set automatically. Restoring the stored brightness (LIM function) to the factory default setting Set the main switch of the microscope frame to (OFF).

  • Page 25: Focusing Unit

    BX53 3-2 Focusing unit List of focusing units The focusing unit consists of the operation units listed below. The following table shows the names and functions of the operation units. Name Function Coarse focusing Rotates to move the focal position knob significantly.

  • Page 26: Replacing The Fine Focusing Knob

    Replacing the fine focusing knob The fine focusing knob is attached on the right side as a factory default. The fine focusing knob is designed to be detachable. NOTE Normally, attach the fine focusing knob on the opposite side of the X-axis /Y-axis knob of the stage. This prevents your hand from contacting the fine focusing knob when operating the X-axis /Y-axis knob.

  • Page 27: Using The Pre-Focusing Lever

    BX53 Using the pre-focusing lever Using the pre-focusing lever controls the vertical movement of the stage no further than the arbitrary position when rotating the pre- focusing lever. With this function, the approximate focal position can be reproduced or the collision between the stage and the objective can be prevented.

  • Page 28: Stage

    3-3 Stage Placing the specimen If you use a slide glass, be sure to use a slide glass of the following size. Slide glass Size 26 x 76 mm Thickness 0.9 to 1.2 mm · When observing a specimen larger than above, remove the specimen holder and place the specimen directly on the stage.

  • Page 29: Using The Auxiliary Specimen Holder

    BX53 When using the specimen hold plate (CX3-SHP) For attaching procedures, see "Attaching the specimen holder" (page 59). Rotate the coarse focusing knob a to lower the stage sufficiently. Place the specimen on the resin sheet b . · As the outside of the aperture c of the resin sheet is not...

  • Page 30: Adjusting The Tension Of X-Axis / Y-Axis Knob

    Adjusting the tension of X-axis / Y-axis knob Hold the X-axis knob a and when you raise the Y-axis knob b , the adjustment knobs c and d appear. Rotating the X-axis adjustment knob c or the Y-axis adjustment knob d in the arrow direction increases the tension and rotating in the opposite direction decreases the tension.

  • Page 31: Rotating The Stage

    BX53 Stage knob extension adapter (BX3-SHEA) Fitting this extension adapter to the X-axis / Y-axis knob of the stage allows the knob operation at the lower position and reduces the fatigue on the arm caused by long hours of operation.

  • Page 32: Observation Tube

    3-4 Observation tube Adjusting the tilt Hand position (right and left) This function is available when U-SWETTR-5, U-TTR-2, U-TBI-3, U- TTBI, U-ETBI or U-TTLBI is combined. You can adjust the eyepieces to an easy-to-see height and angle so that you can observe with a comfortable posture. Eyepiece adjustment range Observation Angle...

  • Page 33: Using The Eye Shades

    BX53 Using the eye shades When wearing eyeglasses Use the eye shades in the folded-down position. When not wearing eyeglasses Raising the folded-down eyeshades in the arrow direction prevents the unnecessary light from entering between eyepieces and eyes. Selecting between the eyepiece light path and the camera light path You can select the light path for observing with the eyepiece or the light path for observing with the display, etc.

  • Page 34: Condenser

    3-5 Condenser For the centering of the condenser, see "Centering the field diaphragm (FS)" (page 11). Swinging-out the top lens of the condenser When using the swing-out type condenser for observation using the 1.25X to 4X objective, swing-out the top lens of the condenser, fully open the aperture diaphragm and use the field diaphragm in the base as an aperture diaphragm.

  • Page 35: Oil Immersion Objective

    BX53 3-6 Oil immersion objective Using the oil immersion objective Apply the specified oil (immersion oil) to the tip of the oil immersion objective. Otherwise, the image is not viewed clearly. Always use the immersion oil made by us. NOTE Using immersion oil which is not provided by us cannot deliver the intended optical performance.

  • Page 36: Objective With Correction Collar

    3-7 Objective with correction collar This function is available when the objective equipped is combined with the correction collar. The cover glass thickness must be matched with the scale value of the correction collar to deliver the full performance of the objective. When the cover glass thickness is known 0.17 Rotate the correction collar a to match the scale value of the cover...

  • Page 37: Observation Procedures Other Than Transmitted Light Brightfield Observation

    BX53 Observation procedures other than transmitted light brightfield observation 4-1 Simple polarization observation procedures Main switch (page 10) Turn ON the main switch. Brightness control knob of microscope frame Adjust the brightness. (page 16) Light path selection lever of trinocular tube Select the light path.

  • Page 38: Adjusting The Crossed Nicol

    4-2 Adjusting the crossed Nicol Setting the analyzer Using the analyzer for transmitted light (U-ANT) Loosen the fixing knob a of the revolving nosepiece to remove the dummy slider b . Face the display surface of the analyzer for transmitted light c up, align the index d with the groove e of the mount of the dummy slider b and fit the analyzer for transmitted light into the mount of the dummy slider b .

  • Page 39: Setting The Polarizer

    BX53 Setting the polarizer When the condenser equipped with a polarizer is attached Engage the polarizer built in the condenser in the light path. The following types of condensers are equipped with polarizers. · Universal condenser (U-UCD8-2) · Motorized universal condenser (BX3-UCD8A) ·...

  • Page 40: Fluorescence Observation Procedures

    4-3 Fluorescence observation procedures For details of the fluorescence observation, refer to the instruction manual provided with the unit. 4-4 Phase contrast observation procedures For details of the phase contrast observation, refer to the instruction manual provided with the unit. 4-5 Differential interference contrast observation procedures For details of the differential interference contrast observation, refer to the instruction manual provided with the unit.

  • Page 41: Image Acquisition

    BX53 Image acquisition Attaching the camera adapter and the microscope digital camera to the trinocular tube allows you to acquire the observed image. The image acquisition range is determined by the size of the image sensor used in the camera and the magnification of the camera adapter.

  • Page 42: Replacement Of The Eyepiece And The Objective

    Replacement of the eyepiece and the objective 6-1 Removing and attaching the eyepiece Removing the eyepiece Pull out the eyepiece a from the eyepiece sleeve b . Attaching the eyepiece micrometer You can use the eyepiece micrometer to check the size of the observed image or the center position of the observation field of view, etc.

  • Page 43: Attaching The Eyepiece

    BX53 Attaching the eyepiece Insert the eyepiece a into the eyepiece sleeve b until it touches the end. · As the binocular tube does not have a positioning groove, NOTE it cannot be used with an eyepiece equipped with a positioning pin.

  • Page 44: Replacing The Objective

    6-2 Replacing the objective When replacing the objective, remove the revolving nosepiece from the standard arm (or fluorescence illuminator) before replacing the objective. If you attach the objective without removing the revolving nosepiece, the objective will be screwed upward (direction opposite to gravity).

  • Page 45: Attaching The Revolving Nosepiece

    BX53 Attaching the revolving nosepiece Rotate the coarse focusing knob to lower the stage sufficiently. Loosen the revolving nosepiece clamping screw using an Allen screwdriver. If the clamping screw is loosened too much, the revolving NOTE nosepiece is caught with the clamping screw and it cannot be attached.
  • Page 46 Connect the cable coming out of the back of the microscope frame to either one of following units. Attaching the coded nosepiece · Microscope frame (BX53F2) · Interface for coded nosepiece (U-IFRES) Attaching the motorized nosepiece · Control box (BX3-CBM) For procedures to connect to BX53F2 in detail, see “Connecting cables”...
  • Page 47 BX53 MEMO...

  • Page 48: Troubleshooting

    Troubleshooting If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact us for assistance. 7-1 Optical systems Problem Cause Remedy Page a) The light source does not turn The cable is disconnected.
  • Page 49 BX53 Problem Cause Remedy Page e) The observed image glares. The aperture diaphragm is narrowed Adjust the aperture diaphragm down too far. according to the numerical aperture 15, 50 of the objective to use. The condenser is lowered too much.
  • Page 50 Problem Cause Remedy Page g) One side of the observed image The revolving nosepiece is not Push in the revolving nosepiece is blurred. attached correctly. along the mount dovetail until it touches the end, and secure it. The stage is not mounted correctly. Mount the stage correctly.

  • Page 51: Electrical Systems

    BX53 7-2 Electrical systems Problem Cause Remedy Page a) The pilot indicator does not turn The cable is disconnected. Connect the cable completely. The power cord is unplugged. Connect the power cord to a power outlet securely. b) A beep is heard five times and...

  • Page 52: Observation Tube

    7-4 Observation tube Problem Cause Remedy Page a) The fields of view of two eyes do The interpupillary distance is not Adjust it correctly. not coincide. adjusted correctly. The difference in diopter of two eyes Correct it properly. is not corrected properly. Different eyepieces are used for right Use the same eyepiece for right side side and left side.

  • Page 53: Specifications

    BX53 Specifications Item Specifications Optical system UIS2(UIS) optical system (infinity correction) Illumination Kohler illumination optical system system High intensity and high color rendering LED (Average life time: Approximately 50,000 h in rated use) LED current adjustment range: 0 mA to 680 mA (continuously variable)
  • Page 54 Item Specifications Observation tube Product name U-TTLBI U-SWTR-3 U-ETBI U-ETR-4 U-SWETTR-5 Widefield tilt & Widefield upright Super widefield Super widefield Widefield upright Model type t e l e s c o p i c l i f t tilt & telescopic upright tilting trinocular tube trinocular tube...
  • Page 55 BX53 Item Specifications Condenser Product name BX3-UCD8A U-UCD8-2 U-AAC U-SC3 · Achromat / Aplanat · Achromat / Aplanat Model type Achromat / Aplanat Swing-out · Motorized swing-out · Swing-out 1.4 (when using U-TLO), N.A. 0.9 (when using U-TLD) Aperture Motorized open/...

  • Page 56: Optical Performance List <

    Optical performance list <<UIS2 series>> Objective series Magnification of objective (PL is described as Plan) The following table shows the combined optical Numerical aperture (NA) performance of the objective and the eyepiece. Mechanical tube length : Infinity Ph: Phase contrast The picture on the right shows the various Oil: Oil immersion performances indicated on the objectives.
  • Page 57 BX53 Eyepiece WHN10X(FN22) Working Cover glass Optical performance Numerical distance thickness Actual aperture (mm) (mm) field of Total Series name Notation magnification view (mm) UPLXAPO UPlanXApo Plan Apochromat 0.16 13.0 (OFN 26.5) * 0.40 0.17 100X 0.80 0.17 200X 0.95 0.18...

  • Page 58: Assembly

    10 10 Assembly 10-1 Assembly diagram The numbers in the following diagram represent the order to attach each unit. The units shown in the following diagram are typical units. For units which are not described below, contact us or refer to the latest catalogs. In order to deliver our intended performance, we recommend you to ask us to assemble the microscope.

  • Page 59: Assembly Procedures

    BX53 10-2 Assembly procedures Assemble the units using the Allen screwdriver (opposite side: 3 mm ) and the Allen wrench (opposite side: 4 mm ) provided with the microscope. Use the Allen screwdriver if not specified. Before assembling the microscope, remove dust and dirt from the mount of each unit and assemble NOTE carefully so as not to scratch them.
  • Page 60 Rotate the coarse focusing knob to raise the focusing unit until the stopper screw b of the arm is visible. Loosen the stopper screw c and remove it. Retain the removed screw in a safe place. Attach the stage holder, push it to the stopper screw at the bottom and tighten the clamping screw.

  • Page 61: Attaching The Condenser

    BX53 Attaching the condenser Rotate the coarse focusing knob to raise the stage holder a to the upper limit. Rotate the condenser height adjustment knob to lower the condenser holder b sufficiently. Loosen the condenser clamping knob sufficiently. Insert the condenser along the dovetail of the condenser holder b from the front side and push it until it touches the end.
  • Page 62 Attaching the auxiliary lens to the condenser U-LC When the low magnification condenser (U-LC) is combined with the microscope, the auxiliary lens must be attached. The auxiliary lens is provided with the low magnification condenser (U-LC). Fit the mounting tool b (provided with U-LC) into the groove a on the lamp adapter on the back of the microscope frame, and rotate the tool counterclockwise to remove the lamp adapter c .

  • Page 63: Attaching The Stage

    BX53 Attaching the stage When replacing the stage from the assembled NOTE microscope, remove the objective together with the revolving nosepiece in advance. Rotate the condenser height adjustment knob to lower the condenser holder a to the lower limit. While looking at the backside of the stage, loosen the stage clamping screw b until the tip of the stage clamping screw is hidden (until there is no feeling of protrusion when touched by fingers).
  • Page 64 Attaching the specimen holder The attaching procedures are different between the regular specimen holder and the specimen hold plate (CX3-SHP). When using the regular specimen holder Rotate the coarse focusing knob to lower the stage sufficiently. Loosen the specimen holder fixing knob a (2 pcs.) of the stage plate. You can also loosen the specimen holder fixing knob a by inserting a coin into the groove of the specimen holder fixing knob a and rotating it in counterclockwise direction.
  • Page 65 BX53 When using the specimen hold plate (CX3-SHP) Rotate the coarse focusing knob to lower the stage sufficiently. Loosen the specimen holder fixing knob a (2 pcs.) of the stage plate to remove them. You can also loosen the specimen holder fixing knob a by inserting a coin into the groove of the specimen holder fixing knob a and rotating it in counterclockwise direction.
  • Page 66 Attaching the standard arm Use the dedicated Allen wrench ( ) provided with the standard arm for attaching the standard arm (BX3-ARM). Place the standard arm on the arm mount of the microscope frame. (At that time, screw holes a (4 positions) of the standard arm slightly come to the front side of screw holes b (4 positions) of the microscope frame.) Slide the standard arm backward until it stops.
  • Page 67 BX53 Adjust the direction of the observation tube so that the value of the interpupillary distance adjustment scale on the observation tube faces to the front, and tighten the clamping screw d of the standard arm to secure it. If the observation tube moves when you attempt to rotate NOTE it, the observation tube is not fixed properly.
  • Page 68 Attaching the LBA filter Up to three LBA filters (46S-LBA4) or filters with the screw of the following size can be attached to the LED Lamp house (U-LHLEDC100). Attachable filter Size Number of filters M46 x 0.75 Three filters Rotating the LBA filter in the arrow direction as shown in the picture, insert it in the LED Lamp house (U-LHLEDC100).

  • Page 69: Setting The Dip Switch

    BX53 Setting the dip switch When the power of the microscope is turned ON, the beep sound is heard. With the dip switch on the back of the microscope frame, you can select not to allow this beep sound heard.

  • Page 70: Connecting Cables

    Connecting cables · Before connecting or disconnecting cables, set the main switch to (OFF) and unplug the power cord NOTE from the outlet. · This product contains the motorized parts. For safety purposes, connect the power cord plug last. · Cables are vulnerable when bent or twisted. Never subject them to excessive force. ·...

  • Page 71: Connecting The Power Cord

    BX53 Layout of the cable for coded nosepiece Attach the cable holder on the back of the microscope ( a position). Open the cable holder a , put the cable for coded nosepiece between the cable holder and close the cable holder.

  • Page 72: Proper Selection Of The Power Supply Cord

    Proper selection of the power supply cord If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Specifications” and “Certified Cord” below: Caution : In case you use a non-approved power supply cord for our products, we can no longer warrant the electrical safety of the equipment.
  • Page 73 BX53 Table 2 HAR flexible cord Approval organizations and cordage harmonization marking methods Alternative marking utilizing Printed or embossed black-red-yellow thread (Length harmonization marking (May be of color section in mm) Approval organization located on jacket or insulation of internal wiring)

  • Page 74: Request To Issue The Safety Confirmation

    11 11 Request to issue the Safety Confirmation NOTE To ensure operational safety of this product, the Safety Confirmation shown on the next page must be issued by the user when the user has this product maintained, repaired or serviced by Evident Corporation.
  • Page 75 Identification number Safety Confirmation when requesting repair/inspection This safety confirmation is for asking you to fill out to ensure the health and safety of service personnel who pick up, repair, and/or inspect the products. Your cooperation would be highly appreciated. Please note if the safety of service personnel cannot be ensured, your requests will not be accepted.
  • Page 76 #04-04/05, 25 Ubi Rd 4, UBIX Singapore 408621 Evident Australia PTY LTD Level 4, 97 Waterloo Road Macquarie Park NSW 2113, Australia Life science solutions Industrial solutions Service Center Service Center https://www.olympus-lifescience.com/ https://www.olympus-ims.com/ support/service/ service-and-support/service-centers/ Official website Official website https://www.olympus-lifescience.com https://www.olympus-ims.com...

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