Optimizing The Detector Performance; Match The Flow Cell To The Column - Agilent Technologies G1314B VWD User Manual

5 How to optimize the detector

Optimizing the Detector Performance

The detector has a variety of parameters that can be used to optimize
performance.
The information below will guide you on how to get the best detector
performance. Follow these rules as a start for new applications. It gives a
rule-of-thumb for optimizing the detector parameters.

Match the Flow Cell to the Column

Figure 26
than one selection is appropriate, use the larger flow cell to get the best
detection limit. Use the smaller flow cell for best peak resolution.
Column length Typical peak
width
<= 5 cm 0.025 min
10 cm 0.05 min
20 cm 0.1 min
>= 40 cm
0.2 min
Typical flow rate
Internal column diameter
Figure 26
66
recommends the flow cell that matches the column used. If more
Micro
flow cell
0.05 – 0.2 ml/min
1.0 mm
Choosing a Flow Cell
1200 Series Variable Wavelength Detector User Manual
Recommended flow cell
Semi-micro
flow cell
Standard
flow cell
0.2 – 0.4 ml/min 0.4 – 0.8 ml/min
2.1 mm 3.0 mm
1 – 2 ml/min
4.6 mm