Olympus Fluoview-1000 User Manual page 4

V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
FV-1000 Optical Specifications
No Image! .................................................................................................................................................
Start-up problems. ..................................................................................................................................
Scans, but no image.
The confocal image appears unevenly illuminated:
The epifluorescent or tungsten images appear unevenly illuminated:
Figure 1. Main Fluoview window.
Figure 2. Enlarged view of Fluoview windows.
Figure 3. Microscope Controller Window
Figure 4. Image Acquisition Window - Basic controls for Finding the Image
Figure 5. Channel Settings for the Photomultiplier Tube (PMT)
Figure 6. The Scan Mode and Scan Size settings of the Acquisition Setting Window.
Figure 7. Selecting laser lines and setting laser power
Figure 8. Scan Controls for Zoom, Pan and Rotation
Figure 9. Sequential Imaging Control
Figure 9A. Sequential Imaging Control
Figure 10. Z-Series Control Window
Figure 11. Z-Series Control Window
Figure 12. DIC and Focus Controls, Lower Half of the Microscope
Figure 13. Condenser and DIC Controls
Figure 14. Tilting the condenser.
Figure 15. The Stimulus Setting window for photoactivation
Figure 16. Photobleaching an ROI.
Figure 17. The toolbar item for control over display color
Figure 18. Fluoview appearance in Darkroom Color
Table 1. Mirror Buttons
Table 2. Objective Lenses Available
Table 3. Scan Speed and Acquisition Time
Table 4. Optimal Z-Step Values
Table 5. Pixel Size (µm) versus Box Size and Magnification at Zoom = 1
Table 6. Dichroic Mirrors and Barrier Filters
Table 7. Laser Lines on the FV-1000
Table 8. Index of Refraction for Common Mounting Materials
May 11, 2011
Olympus Fluoview-1000 User's Guide
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Figures
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