Related Manuals for Olympus Fluoview FV300
Summary of Contents for Olympus Fluoview FV300
- Page 1 CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW...
- Page 2 FV300 CONFOCAL LASER SCANNING MICROSCO PERFORMANCE FOR THE PERSONAL USER The FV300 is the ideal choice of laser scanning microscopes for personal users. Its optical system is fully integrated, from scanner to microscope, and not only delivers outstanding optical sectioning, but also ensures the easy, flexible expandability required for any future upgrade.
- Page 3 q Optical fiber for laser introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil lens i Collector Lens o Pinhole turret !0 Emission beam splitter slider !1 Barrier filter slider !2 Photo multiplier...
- Page 4 Software Graphical User Interface Ultimate ease of operation and monitor display. Dye selection display Versatile display options Preset the conditions for image acquisition and loading When a fluorescence dye is chosen, the Exchange between condensed and full laser and light path settings are selected image display modes can be performed Storage of Acquisition Settings enables automatically, with each of the selected...
- Page 5 Sequential scanning to prevent cross talk glance comparison of multiple images Sequential scanning may be used to minimize the fluorescence cross talk The FV300's live tiling function, which is often seen between channels in multicolored samples. This is achieved by especially valuable in time course exciting each fluorochrome independently, one dye at a time.
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Page 6: Time Course
Superior slice patching system In combination with the unique fixed stage & nosepiece focusing BX61WI microscope, the FV300 provides a highly effective system for slice patching. This unique set- up has a small footprint for increased room in a space-limited cage. The remote control... - Page 7 (optional time course Cameleon genes provided by Dr. Miyawaki Atsushi in Brain Research Institute. software: TIEMPO) Equipment: FV300 and HeCd laser Time period : 4 seconds. CFP Fluorescence wavelength 485nm YFP Fluorescence wavelength 530nm Input/output of external trigger signal...
- Page 8 PAPP for FRAP Application PAPP: Programmable Acquisition Protocol Processor Easy, reliable flow of experiments for fluorescence recovery after photobleaching. AOTF and PAPP function for effective Mouse; hippocampal neurons; fluorescence of GFP FRAP (Fluorescence Recovery After Living neurons expressing GFP were maintained in Photobleaching) culture and fluorescent images were obtained.
- Page 9 Multi-point time lapse system High-magnification multi-point time lapse observation of living cells. Wide-ranging specimen observations for improved experiment throughput Use of a motorized XY stage allows the analysis of time lapse changes in many points scattered over a wide area. The system is therefore effective for work with thick specimens, such as observing changes in the states and movements of...
- Page 10 3D Imaging Using multiple 3D images to obtain accurate 3D structure analysis. X-Y-Z X-Y-Z ZoomIn-Z X -Z Y -Z SlantLine-Z FreeLine-Z Easy Z axis operation and setting The upper and lower limit of Z scanning can be specified interactively by actually scanning the sample or by direct input of the numerical value.
- Page 11 Physiolink software time scale. • The patch clamping graph and Physiolink software are interlocked and activated concurrently. • Physiolink software complies with the FV300’s high-speed scanning, enabling msec analysis. Ethernet Physiolink software (FV300 side) Physioview software (Axon PC side) Axon...
- Page 12 Applications Gallery Lucifer Yellow: retina ganglion cell TexasRed: dopamine-operated amacrine cell Prof. Shigetada Nakanishi Dept. of Biological Sciences, Kyoto Univ. Faculty of Medicine Mouse hippocampal neurons Purkinje cell in the rat cerebellum GFP: postsynaptic density protein FITC: vesicular GABA transporter VGAT Rhodamine-phalloidin: actin Cy3: vesicular glutamate transporter VGLUT1 Hippocampal neurons expressing a GFP-tagged...
- Page 13 GFP-labeling of Drosophila adult brain with staining of mushroom bodies Assistant Prof. Aigaki Cytogenetics Tokyo Metropolitan University, Science Dept. C elegans expressing beta-integrin fused to GFP Expression of DsRed in a zebrafish embryo Dr. Xioping Xhu and Dr. John Plenefisch Extended focus image of 5µmx30 slice University of Toledo, Dept.
- Page 14 Fluorescence Dyes and Filters BA530RIF BA550RIF BA650RIF BA430-460 BA465-495 BA470-520 BA480-495 BA480-510 BA505-525 BA505-550 BA510-540 BA520-550 BA535-565 BA540-590 BA560-600 BA585-615 BA585-640 BA510IF BA560IF BA565IF BA585IF BA590 BA610IF BA625IF BA660IF Kaede ECFP EGFP FITC EYFP Propidium Iodide Rhodamine-Phalloidin 552 565 DsRed Texas Red Excitation Emission...
- Page 15 Fluorescence illumination unit Connect to external mercury light source and microscope via fiber cable Standard equipment of FV300-BX51, FV-300-BX61, FV300-BX51WI, FV300-BX61WI PC with system control boards PC-AT compatible machine/OS: Windows XP (English version)/ 1GB memory (can be expanded to a maximum of 4GB) CPU: Pentium 4, over 2.8GHz, Special I/F board/image capture: PCI bus...
- Page 16 *Direct fiber connection to scan unit. •Standard configuration for FV300-BX51/FV300-BX61/ •Installation stand is not included in the unit. FV300-BX51WI/FV300-BX61WI combination. * Please consult your Olympus dealer for additional laser combinations. All brands are trademarks or registered trademarks of their respective owners. Monitor images are simulated.