Olympus Fluoview-1000 User Manual page 3

V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
Bleedthrough. .........................................................................................................................................
Reducing bleedthrough
Procedure to minimize bleedthrough
Noise. .....................................................................................................................................................
Signal Noise (Shot Noise).
Detector Noise. ..................................................................................................................................
Kalman Averaging.
Background ............................................................................................................................................
Bright Images versus Scorching the Specimen
Images with Weak Signals
Using Zoom ...........................................................................................................................................
Zoom reduces objective lens aberrations.
Use Zoom to fulfill sampling requirements for the specimen.
Use Zoom to fulfill adequately sample the objective.
Obtaining a Z-Series.
Resolution. .............................................................................................................................................
Sampling frequency and the Nyquist Theorem.
Box size. ............................................................................................................................................
Zoom. 38
Considerations for increasing resolution.
Size of Pixels .........................................................................................................................................
Table. 5 Pixel Size (µm) versus Box Size and Magnification at Zoom = 1
Microscope controls.
Focus. .....................................................................................................................................................
Fine/Coarse Focus.
Lowering the Objectives.
Manual shutter. ..................................................................................................................................
Differential Interference Contrast (DIC).
DIC Analyzer. ....................................................................................................................................
DIC polarizer. ....................................................................................................................................
DIC mirror. ........................................................................................................................................
Setting Koehler Illumination.
Condenser position.
Photoactivation Methods.
Zoom versus Laser Power.
Recording initial recovery event.
Methods to photoactivate/bleach.
Using the Stimulus Window.
Avoid spontaneous photoactivation.
Controlling Computer Display Brightness.
Set 'Darkroom' color mode.
Technical Information
Caring for Objectives
May 11, 2011
Olympus Fluoview-1000 User's Guide
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