Olympus FV3000 Operation Manual
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Olympus FV3000 Operation Manual

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Operation Manual

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  • Page 1 FV3000 Operation Manual...

  • Page 2: Table Of Contents

    Contents -----------------Image Acquisition--------------- Starting the System ――――――――――――――――― 4 Visual observation with microscope ―――――――――― 5 XY Image Acquisition ――――――――――――――――― 6 XYZ Image Acquisition ―――――――――――――――― 8 XYT Image Acquisition ―――――――――――――――― 9 4D（XYZT）Image Acquisition ―――――――――――― 10 XYT Image Acquisition（with Z drift compensation） ―― 11 Multi Area Time Lapse ――――――――――――――――...

  • Page 3: Image Acquisition

    Image Acquisition...

  • Page 4: Starting The System

    ②Central Factory Default ID & password power Factory User ID：olympus Password：olympus ④Touch Panel Controller（TPC) Starting software for FV3000 ⑦ Double-click the [FV31S-SW] icon on the desktop. ※Start the software after “Start operation” TPC main is displayed in TPC. switch ⑦...

  • Page 5: Visual Observation With Microscope

    Visual observation with microscope ★Fluorescence observation ★Transmitted DIC observation *Tap the button of the TPC to select *Tap the button on the TPC to the objective for observation. select the objective for observation. ① Tap the light path selection button ①...

  • Page 6: Xy Image Acquisition

    XY Image Acquisition(1) ① Select “VBF” in the [PMT Setting] Tool Window. ① Assigning the detector to channel ② Press the button on [PMT Setting] ⑧ ② Tool Window to open the [Dye & Detector Select] ⑩ dialog box. Reducing noises by Averaging.
  • Page 7 Slide 6 確認（英語のほう） 元島 ゆき; 03.02.2017...
  • Page 8 XY Image Acquisition(２) Setting the scanner ⑩ On [LSM Imaging] Tool Window, select the scan method in [Type] and [Mode]. ⑩ ※See 23 pages for details. ⑪ Set scanning speed. ⑪ ⑫ Specify “Image Size” to set the scan area. ⑬...

  • Page 9: Xyz Image Acquisition

    XYZ Image Acquisition *Before starting the following procedure, make adjustments for XY imaging. (refer to page 4-5.) ① Setting Z series ① Select “ON” in [Z] on [Series] Tool Window. ② ② Select “Start/End” in [Motor] on [Z section]. Pressing the button, current position is registered as origin.
  • Page 10 Slide 8 新しく追加。要チェック。 元島 ゆき; 08.02.2017 Step Sizeにしたけど大丈夫だろうか？ 内容・英語ともに。 元島 ゆき; 08.02.2017...

  • Page 11: Xyt Image Acquisition

    XYT Image Acquisition *Before starting the following procedure, make adjustments for XY imaging. (refer to page 4-5.) ④ Setting Time Series ⑤ ① Select “ON” in [Time] on [Series] Tool ③ Window. ② Set the interval to acquire the image in [Interval] and [Cycle] on [Time Lapse].
  • Page 12 4D Image Acquisition *Before starting the following procedure, make adjustments for XY imaging. (refer to page 4-5.) ① Select “ON” in both [Z] and [Time] on ⑦ [Series] Tool Window. Setting Z series ⑧ ② Changing the Z position, register the Z position of Start/End.

  • Page 13: Xyzt）Image Acquisition

    ◆ZDC◆ XYT Image Acquisition (with the Z drift compensation） ＊In advance, prepare to use ZDC. （See page 22 for details. ） ＊Set for acquiring the series image. ⑤ Setting Near limit ⑥ ③ Select [Microscope] in [Tool Window] menu. Press the button to set the Near limit to all objective lenses in [Z limit Setting].

  • Page 14: Multi Area Time Lapse

    Multi Area Time Lapse ◆motorized stage◆ Imaging Acquisition *Before starting the following procedure, ① make adjustments for XY imaging. ① Press the button in [Live] Window. ② Select the [Map] sub pane. Registering the group of MATL ② ③ Move the specimen to a desired position and adjust the live image.
  • Page 15 Multi Area Time Lapse ◆motorized stage◆ ◆ZDC◆ （with the Z drift compensation） *Before starting the following procedure, make adjustments for XY imaging. ZDC Setting (See page 10 for details. ) ① In [▼Z Limit Setting], press the button to set the Near limit to all objective lenses.

  • Page 16: Multi Area Time Lapse Using Map Image（１

    Multi Area Time lapse ◆motorized stage◆ using Map Image（１） *Before starting the following procedure, make adjustments for XY ① imaging. ① Press the button in [Live Window], the sub pane appears. ② Select [Map] tab. ② Create the map ③ ③...
  • Page 17 Multi Area Time lapse ◆motorized stage◆ using Map Image（２） Registering the multiple area ⑨ Set the higher magnification objective lens. double-clicking on the map image, the stage is moved to at the center of the ⑨ map. ⑩ Press the to register the position and acquiring parameters.
  • Page 18 Acquiring the stitched image using Map Image ① Refer to page 12, acquiring the map image. Define the area of acquisition ② Move the specimen to desired position using the map image as reference. Double-clicking on the map, the motorized stage moves to the position where double-clicked.

  • Page 19: Tips Of Image Acquisition

    Tips for Image Acquisition ■For the High speed scanning■ *Check whether [Scan Speed] is set “2.0μs/pixel”. １）Changing the scanner ＊Use the Resonant scanner ＊Use the Roundtrip Mode When using High speed scanner, the signal gets weaker and the noise increases. So, refer to [■For brighter imaging■] and [■For the lower noises■] and combine them.

  • Page 20: Exiting The System

    Exiting the system Touch Panel Controller（TPC) Exiting the software and PC ③Touch ① Select [Exit] in the [File] menu to the ”OFF” exit the software. ② Select the “Shut down” in Windows ④TPC main Start Menu. switch ① ② Turning OFF the power Touch Panel Controller（TPC) ③...

  • Page 21: Configuration

    Configuration Changing the objective lenses ① ＊Exchange the objective lens to be used. ① Select [Configuration] in [Tools] menu. The [Configuration] dialog box appears. ② Select [Microscope] tab. ② ③ Select [Objective Lens]. ④ Select the name of the mounted ③...

  • Page 22: Processing Image

    Processing Image...

  • Page 23: 2D View And Operation

    2D view and operation Drawing scale bar and ROI Playing animations of the series images ・A straight line can be drawn by pressing ・These buttons are used to play Shift key when left-clicking. animation of the series images. All delete Set the range play Frame late...
  • Page 24 3D view and operation Opening the file and displaying 3D image Open and activate the Z series image. Select [Volume] tub to display 3D image. 3D image setting Press the button and select [Volume Setting] in the [Tool Window] menu. [Volume Setting] Tool Window is displayed.

  • Page 25: Creating The Movies

    Creating the movie *Opening the file and displaying 3D image ① ① Press the button and select [Volume Setting] in the [Tool Window] menu. ② Select [Movie] tab in [Volume Setting] Tool Window. ③ Select “▼Key Frame” in [Movie Item] and press the button.

  • Page 26: Processing: Projection

    Processing: Projection ① Press the button to switch to ② ① “Viewer mode”. ② Select [Analysis] in the [Tool Window] menu. ③ Select “Single process” and “post processing” as the illustration below. ④ Select the [Processing] tab and register “projection” in[ Processing Item]. ③...

  • Page 27: Exporting The Image

    Exporting the image *The image can be exported in the file format which can be used by other software. A. Exporting a single image ① Right-click on the image to be exported. Select [Export] in the menu displayed. The [Export] dialog box appears. ②...

  • Page 28: Reloading And Saving Observation Methods

    Reloading and saving Observation Methods Save / load the observation method ① ① Select the [Observation Method] in [Tool Window] menu. ＊Each functions :to load the acquisition parameters and set to FV :to save the current conditions and add to the list :to update the current conditions :to delete the condition from the list : to sort the list...

  • Page 29: Spectral Imaging

    Spectral Imaging...

  • Page 30: Lambda Series（Acquiring By Single Channels

    Lambda series （acquiring by a single channels） *Before starting the following procedure, ⑤ make adjustments for imaging. (refer to page 4-8.) ⑥ ⑦ Changing to Lambda mode ① Select “Lambda” in [Mode] in [PMT setting] Tool Window. Select one channel to be used Figure(A) ②...
  • Page 31 Lambda series （acquiring by a single channels） Setting the wavelength ⑨ Set them by dragging the mouse on the profile display area or set the value of wavelength directly to [CH1].（★） ⑫ Adjusting the live image ⑩ Press the button to start scanning. ⑪...

  • Page 32: Lambda Series（Acquiring By Multiple Channels

    Lambda series （acquiring by multiple channels） *Before starting the following procedure, make adjustments for XY imaging. ① (refer to page 4-5.) Changing to Lambda mode ② ① Select “Lambda” in [Mode] in [PMT setting] Tool Window. ③ Select one channel to be used ②...
  • Page 33 Lambda series （acquiring by multiple channels） Setting the wavelength ⑨ Set them by dragging the mouse on the profile display area or set the value of wavelength directly to [CH1].（★） *You cannot select the laser which includes ±5nm of the excitation wavelength in the photometry range of each channel.

  • Page 34: Processing: Unmixing（１

    Processing: Unmixing（１） Spectral Image Unmixing 〜by specifying dyes ① Press the button to switch to “Viewer mode”. ② ③ ② Select [Analysis] in [Tool Window] menu. [Analysis] Tool Window ④ appears. ④ ③ Select [Single process] mode, press the [Post Processing] button. ⑤...

  • Page 35: Processing: Unmixing（２

    Processing: Unmixing（２） Blind Unmixing 〜by setting the number of dyes ① Press the button to switch ② ③ to “Viewer mode”. ② Select [Analysis] in [Tool Window] ④ ④ menu. [Analysis] Tool Window appears. ③ Select [Single process] mode, press the [Post Processing] button.

  • Page 36: Processing: Unmixing（３

    Processing: Unmixing（3） Normal Unmixing 〜by specifying dye dates ② ① Press the button to switch ③ to “Viewer mode”. ④ ② Select [Analysis] in [Tool Window] ④ menu. [Analysis] Tool Window appears. ③ Select [Single process] mode, press the [Post Processing] button. ⑤...

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