Olympus Fluoview-1000 User Manual page 2

V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
Scan mode and sequential capture
Using Channel Groups, Figure 9A.
Adjusting the channels with sequential imaging
Adjusting the channels with simultaneous imaging
Collecting a Z-Series.
Determine the depth of the optical volume.
Which way does the focus move?
Settings for z-series collection.
What is axial resolution?
Vertical Sections. ...................................................................................................................................
Files: Saving and Transferring
Saving Files ...........................................................................................................................................
The Olympus "OIB" format.
The Olympus "OIF" format.
Where are Your Files on the Confocal?
Export file types. ....................................................................................................................................
Add a scale bar to your file
Opening your Files.
Olympus free file viewer
Offline Fluoview workstation
Importing Olympus datasets into ImageJ.
Loci-tools Plugin for ImageJ.
Import Channel Order Macro.
Image 5D plugin.
File Import from the OIF.Files directory.
File Transfer. ..........................................................................................................................................
File transfer to Mac Pro 'Fluoview', confocal file server.
Saving files directly to Mac Pro 'Fluoview'.
USB and Firewire Ports on the Mac Pro.
Recording to CD-ROM and DVD-ROM on the Mac Pro.
Networked file transfer to remote servers from the Fluoview Mac Pro
Using FTP to Windows computers
Remote access to the Mac Pro from your laboratory.
Optimizing Image Collection
Qualities of an Optimal Confocal Image.
Overview of image capture properties
Overview of basic image capture.
Basic settings for confocal microscopy
Labeling Controls. .................................................................................................................................
Negative controls
Positive controls
The confocal aperture
Optimal Iris Diameter
May 11, 2011
Olympus Fluoview-1000 User's Guide
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