This short instruction will guide you through the turn on and off sequence of the microscope system. For a detailed instruction in system operation refer to the Olympus user manual. This instruction also contains important information on laser safety and general guidelines.
A reflected laser beam is just as dangerous as a direct beam. 3. Never deactivate the laser protection devices. Read the “Safety Guide” in the Olympus User’s Manual to familiarize yourself with the safety devices of the laser scanning microscope. If you find any of the laser protection devices inactivated, please contact the facility staff immediately.
Olympus FV1000 Short instructions 2. General guidelines 1. Lab rules: This room is a S2 laboratory. At times when S2 experiments are performed in this room, you are not allowed to enter the room. S2 experiments will be indicated by a sign at the outside of the door and in the online booking system.
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Short instructions 5. Changing objectives: Never change the objectives manually! To change objectives use the microscope control menu within the Olympus software or the panel box left of the microscope stand. 6. Changing the specimen: Before you change the specimen, drive down the objective nosepiece.
559 nm 635 nm The lasers for 405, 440, and 635 nm are controlled entirely through the Olympus software. These lasers are switched on by selecting an acquisition protocol from the dye list in the “Image Acquisition” window or by directly clicking on the laser box in the “Acquisition Setting”...
Olympus FV1000 Short instructions 4. Using the climatic chamber 1. Check the water bottle at the control unit behind the microscope table. Refill with distilled water only. Distilled water is in Wolfgang Posselt’s office. Eventually open the valve underneath the water bottle.
Olympus FV1000 Short instructions 5. Switching off the microscope 1. Exit the control software. 2. Shut down Windows. 3. Turn the key switch of the 491/532 nm laser to off (no. 4). 4. Turn the key switch of the 559 nm laser to off (no. 5).