Olympus Fluoview-1000 Manuals

Manuals and User Guides for Olympus Fluoview-1000. We have 1 Olympus Fluoview-1000 manual available for free PDF download: User Manual

Olympus Fluoview-1000 User Manual

Olympus Fluoview-1000 User Manual (50 pages)

Brand: Olympus | Category: Microscope | Size: 35.78 MB

Table of Contents

Table of Contents
1
Quick Start Guide
5
- Start-Up
  5
- Shut-Down
  5
Quick Guide to Getting an Image
6
Setting up for Confocal Microscopy
11
Collecting a Z-Series
23
Files: Saving and Transferring
26
Optimizing Image Collection
31
- Qualities of an Optimal Confocal Image
  31
  - Overview of Image Capture Properties
    31
- Overview of Basic Image Capture
  31
  - Basic Settings for Confocal Microscopy
    31
- Labeling Controls
  32
  - Negative Controls
    32
  - Positive Controls
    32
- The Confocal Aperture
  32
  - Optimal Iris Diameter
    32
- Bleedthrough
  33
  - Reducing Bleedthrough
    33
  - Procedure to Minimize Bleedthrough
    33
- Noise
  34
  - Signal Noise (Shot Noise)
    34
  - Detector Noise
    35
  - Kalman Averaging
    35
- Background
  35
- Bright Images Versus Scorching the Specimen
  35
- Images with Weak Signals
  35
- Using Zoom
  36
- Obtaining a Z-Series
  37
- Resolution
  37
- Size of Pixels
  39
Table. 5 Pixel Size (ΜM) Versus Box Size and Magnification at Zoom = 1
39
Figure 12. DIC and Focus Controls, Lower Half of the Microscope
40
Microscope Controls
41
- Focus
  41
  - Fine/Coarse Focus
    41
  - Lowering the Objectives
    41
  - Manual Shutter
    41
- Differential Interference Contrast (DIC)
  41
  - DIC Analyzer
    41
  - DIC Polarizer
    42
  - DIC Mirror
    42
- Setting Koehler Illumination
  42
Photoactivation Methods
44
Technical Information
48
No Image
50

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