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Olympus FV1200 User guide
Olympus FV1200 MPE Microscope User Guide
A. Microscope Start Up
1. Sign-in to the log book with the start time.
2. Turn on mercury lamp power supply
ON/OFF button and releasing it (The light should lights
up in Blue).
3. Turn on microscope controller
controller
.
4.
Turn on the touch panel controller
Operation".
5. Turn on both scanner controllers (MAIN and SIM)
switch ON and then turn the key ON.
6. Turn on the computer if it is not on.
7. Enter user name/password to log on to Windows7.
by pushing the
and XY stage
and touch "Start
;
June 23, 2015
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Summary of Contents for Olympus FV1200 MPE
- Page 1 Olympus FV1200 User guide June 23, 2015 Olympus FV1200 MPE Microscope User Guide A. Microscope Start Up 1. Sign-in to the log book with the start time. 2. Turn on mercury lamp power supply by pushing the ON/OFF button and releasing it (The light should lights up in Blue).
- Page 2 Olympus FV1200 User guide June 23, 2015 B. Laser start up 1. Turn on laser power supplies a) Multi Ar laser : Switch to ON and turn the Key to ON. b) MCPSU (405/440/473/559/635nm) : Switch ON. ...
- Page 3 Olympus FV1200 User guide June 23, 2015 C. Outline of Acquisition Setting / Image Acquisition/ Image Viewer Windows Before taking images, let’s get familiar with buttons and sliders on the Acquisition Setting Window (such as scan mode, scan speed, image size etc).
- Page 4 Olympus FV1200 User guide June 23, 2015 D. Overview of Image Acquisition Control window : Click Transmission light or Epifluorescent light to turn it on and off. : Click on Focus x2, Focus x4, or XY Repeat begins scanning without image acquisition.
- Page 5 Olympus FV1200 User guide June 23, 2015 E. Viewing with Transmitted or Epifluorescence Light Before scanning the sample with laser light, look at the sample and find the region of interest first with either transmitted or fluorescent light. . Place the slide on AKE SURE THE SLIDE AND COVERGLASS ARE CLEAN AND SEALED ...
- Page 6 Olympus FV1200 User guide June 23, 2015 4. After observation, click or [DIA] button on the TPC to close the shutter. F. Image Acquisition (Multicolor imaging) 1. Click on Dye list button on the Image Acquisition Control Window and double click on the fluorescent dyes you want to observe from the list.
- Page 7 Olympus FV1200 User guide June 23, 2015 6. Set up proper acquisition condition each fluorescence colors by adjusting laser power, PMT detector sensitivity, and offset. The following procedure is for Alexa Fluor 405, EYFP, and Alexa 633 as an example.
- Page 8 Olympus FV1200 User guide June 23, 2015 Sequential Scanning Mode In many cases, the bleed-through between multicolor can’t be eliminated by a spectral setting. It can be avoided by sequentially scanning the sample with one laser and one detector at a time.
- Page 9 Olympus FV1200 User guide June 23, 2015 G. Z-Series (xyz) Image Acquisition Use this mode to obtain optical section through the depth (z dimension) of your sample that can be used for 3D visualization. Before starting the following procedure, make adjustment for xy multicolor imaging condition as described above.
- Page 10 Image folder of a Log-in user, click on button and it will direct to your image folder. Select Olympus Image Binary Format *.oib file type, type file name, and click on Save button. (Log-in user and its associate folder will be created as you become self-user).
- Page 11 Olympus FV1200 User guide June 23, 2015 Check the Save Properties As ASCII Text box to save the metadata that contain information about the image acquisition setting. This data will be useful to find out the imaging condition later with other programs.
- Page 12 Olympus FV1200 User guide June 23, 2015 J. System Shut-Down Procedure Wipe off the oil or water from any oil objectives you have used during your session with lens paper. Logging off and transferring data. 1. Exit the FV10-ASW program.