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Olympus FV1000 Short Instructions

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Download this manual See also: User Manual
Olympus FV1000
Short instructions. Olympus FV1000
Version 110608
This short instruction will guide you through the turn on and off sequence of the microscope
system. For a detailed instruction in system operation refer to the Olympus user manual.
This instruction also contains important information on laser safety and general guidelines.
Content:
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Short instructions
Page 2
Page 3
Page 5
Page 6
Page 7

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Summary of Contents for Olympus FV1000

  • Page 1: Table Of Contents

    This short instruction will guide you through the turn on and off sequence of the microscope system. For a detailed instruction in system operation refer to the Olympus user manual. This instruction also contains important information on laser safety and general guidelines.

  • Page 2: Laser Safety

    A reflected laser beam is just as dangerous as a direct beam. 3. Never deactivate the laser protection devices. Read the “Safety Guide” in the Olympus User’s Manual to familiarize yourself with the safety devices of the laser scanning microscope. If you find any of the laser protection devices inactivated, please contact the facility staff immediately.

  • Page 3: General Guidelines

    2. Booking and keys: The use of the Olympus FV 1000 system is restricted to live cell experiments. Before you can use the microscope you have to make a reservation in the online booking system. Please note in the reservation the desired temperature. If you don’t use the climatisation control not this also in the reservation...
  • Page 4 Short instructions 5. Changing objectives: Never change the objectives manually! To change objectives use the microscope control menu within the Olympus software or the panel box left of the microscope stand. 6. Changing the specimen: Before you change the specimen, drive down the objective nosepiece.

  • Page 5: Switching On The Microscope

     655 nm The lasers for 405, 440, and 655 nm are controlled entirely through the Olympus software. These lasers are switched on by selecting an acquisition protocol from the dye list in the “Image Acquisition” window or by directly clicking on the laser box in the “Acquisition Setting”...

  • Page 6: Using The Climatic Chamber

    Olympus FV1000 Short instructions 4. Using the climatic chamber 1. Check the water bottle at the control unit behind the microscope table. Refill with distilled water only. Distilled water is in Lindloff’s office. Open the valve underneath the water bottle.

  • Page 7: Switching Off The Microscope

    Olympus FV1000 Short instructions 5. Switching off the microscope 1. Exit the control software. 2. Turn the key switch of the 491/532 nm laser to off (number 4). 3. Turn the key switch of the 559 nm laser to off (number 5).