Focus On The Specimen - Nikon ECLIPSE E200 MV Series Instruction Manual

12

Focus on the specimen.

(1) Looking into the eyepieces, turn the
brightness control dial to adjust the
brightness of the viewfield.
(2) If the specimen is out of focus, rotate the
focus knobs to focus on the specimen.
Do NOT turn the right and left focus knobs
simultaneously in opposite directions, and do
NOT turn the coarse focus knob further after
the stage has reached its upper or lower limit.
These operations will damage the focusing
mechanism.
Turning the focus knobs recklessly is a long and
hard way to focus on the image. If you are using a
high magnification objective, you may even
damage the specimen by pressing it against the
objective. Before breaking the coverglasses or
damaging the objectives, read the following and
find the correct way to focus on the specimen.
1. When rotating the coarse focus knob while
looking into the eyepieces, be sure to turn it
only in the direction that lowers the stage.
2. When raising the stage using the coarse
focus knob, take your eyes off the eyepieces
and look at the gap between the upper
surface of the specimen and the front of the
objective from the side.
3. First focus with a low magnification
objective. Then change to a high
magnification objective.
* The next step 13 is for microscopes equipped with field diaphragm.
If the microscope does not have a field diaphragm, skip to step 14.
Chapter 4 Microscopy Procedure (Detailed)
17
(1)
Darkens
(2)
Do NOT turn
the knobs in
opposite directions.
Coarse focus knob
Brightens
4