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4411-0133 Version 2 November 15, 2013 *4411-0133*...
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The information in this publication is believed to be accurate as of the publication release date. However, Princeton Instruments does not assume any responsibility for any consequences including any damages resulting from the use thereof. The information contained herein is subject to change without notice.
Grounding and Safety ....................7 Precautions ........................8 Cleaning ........................8 Repairs .......................... 9 Princeton Instruments Customer Support ..............9 About this Manual ......................9 Chapter 2 System Component Descriptions ..........11 System Components ....................11 PIXIS-XB Camera ...................... 12 PIXIS-XB Power Supply....................
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PIXIS-XB System Manual Version 2 Chapter 6 Advanced Topics ................47 Introduction ........................ 47 Timing Modes ......................48 Fast and Safe Modes....................54 LOGIC OUT Control ....................56 Kinetics Mode ......................57 Custom Modes ......................60 Chapter 7 Troubleshooting ................63 Introduction ........................
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Figure 7. Camera Detection Wizard - Welcome dialog ........... 26 Figure 8. LightField Experiment Workspace..............27 Figure 9. Block Diagram of PIXIS-XB System .............. 29 Figure 10. Initial System Checkout Imaging Experiment Layout ........30 Figure 11. Example of WinView Data Acquired from First Light Procedure ....33 Figure 12.
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PIXIS-XB System Manual Version 2 Figure 43. WinX: Vertical Shift ..................62 Figure 44. Acquisition Display ..................64 Figure 45. Camera1 in Camera Name Field ..............65 Figure 46. Data Overrun Due to Hardware Conflict dialog ..........68 Figure 47. Devices Missing dialog .................. 69 Figure 48.
PIXIS-XB Thank you for purchasing a PIXIS-XB camera system from Princeton Instruments. For over three decades Princeton Instruments has been the legendary name behind the most revolutionary spectroscopy and imaging products for cutting edge research. Among the many state of the art features are its integrated controller, deep thermoelectric air-cooling, and compact design.
Do not block air vents on the camera. Preventing the free flow of air overheats the camera and may damage it. If the PIXIS-XB camera system is used in a manner not specified by Princeton Instruments, the protection provided by the equipment may be impaired. Cleaning WARNING! Turn off all power to the equipment and secure all covers before cleaning the units.
Repairs Save the original packing materials. Because the PIXIS-XB camera system contains no user-serviceable parts, Princeton Instruments must do repairs. Should your system need repair, contact Princeton Instruments Customer Support for instructions (telephone, e-mail, and address information are provided on page 92 of this manual).
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PIXIS-XB System Manual Version 2 Chapter 7, Troubleshooting provides courses of action to take if you should have problems with your system. Appendix A, Specifications includes camera specifications. Appendix B, Outline Drawings includes outline drawings of the camera and the CoolCUBE circulator.
System Components Standard Components A typical air-cooled PIXIS-XB system consists of the camera with a Certificate of Performance, a power supply, a USB 2.0 interface cable for your computer system, MCX to BNC adapter cables, and the user manual. A typical liquid-cooled PIXIS-XB system...
Only scientific-grade devices are used in order to ensure the highest image fidelity, resolution, and acquisition flexibility required for scientific X-ray imaging and spectroscopy. Princeton Instruments has developed exclusive CCDs with unmatched quantum efficiency and low noise to offer the utmost in sensitivity.
Chapter 2 System Component Descriptions Fan: Air-cooled cameras contain an internal fan. Its purpose is: to remove heat from the Peltier device that cools the CCD array and to cool the electronics. An internal Peltier device directly cools the cold finger on which the CCD is mounted.
MCX to BNC Adapter Cables: Two MCX to BNC adapter cables are provided with the PIXIS-XB system. These mount to the EXT SYNC and the LOGIC OUT connectors on the rear of the PIXIS-XB. Certificate of Performance Each PIXIS-XB camera has a Certificate of Performance.
PICam™: The standard 64-bit software interface for cooled CCD cameras from Princeton Instruments. PICam is an ANSI C library of camera control and data acquisition functions. Currently, the interface supports Windows Vista and Windows 7.
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PIXIS-XB System Manual Version 2 Fiber Optic Extender Kit The specially designed fiber optic data interface kit allows the computer and the USB2.0 camera head to be separated by up to 500 meters without the loss of data. The kit consists of two compact, high speed transceivers (interface modules) for completely transparent operation between the host computer and the camera.
Chapter 3 Initial System Verification The list and diagrams below briefly describe the sequence of actions required to install your system and verify operation. Refer to the indicated references for more detailed information. Action Reference 1. If the system components have not already been unpacked, unpack Chapter 4 System Setup, them and inspect their carton(s) and the system components for page 19...
The CCD and coating you ordered have been installed in the camera head. Keep all of the original packing materials so you can safely ship the PIXIS-XB system to another location or return it for service if necessary. If you have any difficulty with any step of the instructions, call Princeton Instruments Customer Support.
Camera and Power Supply CoolCUBE Circulator and hoses (for liquid-cooled system) Host Computer: Can be purchased from Princeton Instruments or provided by user. For enhanced performance, a fast hard drive (10,000 rpm) and 2GB RAM is recommended. Operating System: ...
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Chapter 4 System Setup WinX Requirements ® Windows XP (32-bit with SP3 or later) or Vista (32-bit) ® 2 GHz Pentium 4 (or greater). Native USB 2.0 support on the mother board or USB 2.0 Interface Card (Orange Micro 70USB90011 USB2.0 PCI is recommended for desktop;...
3. Reboot the computer. 4. At bootup, Windows will detect the Princeton Instruments USB2 Interface hardware in the PIXIS-XB. You may be prompted to enter the directory path(s) for the apausbprop.dll and/or the apausb.sys file(s), either by keyboard entry or by using the browse function.
Chapter 4 System Setup LightField The following installation is performed via the LightField software installation CD. 1. Before starting the installation: Verify that the computer operating system is Windows Vista (64-bit) or Windows 7 (64-bit). Confirm that your computer supports USB 2.0. If it does not, please refer to the manufacturer’s instructions for installing a USB 2.0 interface card.
1.00” [25.4 mm] or 1.55” [39.2 mm]. Figure 6. Position of Camera Mounting Hole Princeton Instruments does not supply a vacuum interface flange but has provided a dimensioned drawing with the minimum design requirements for a customer-supplied...
Chapter 4 System Setup Making the Camera-Circulator Connections for a CoolCUBE For liquid-cooled cameras, the CoolCUBE circulator provides a vibration-free method of heat removal. 1. Make sure the camera and the circulator power switches are turned off. 2. Make sure the circulator is 6 inches (150 mm) or more below the camera.
1. Make sure the PIXIS-XB is connected to the host computer and that it is turned 2. Run the WinX application. The Camera Detection wizard will automatically run if this is the first time you have installed a Princeton Instruments WinX application (WinView/32, WinSpec/32, or WinXTest/32) and a supported camera.
Chapter 4 System Setup Figure 8. LightField Experiment Workspace 5. Because this is a new experiment, the default settings will automatically be entered for the experiment device(s). These settings will allow you to begin previewing (Run button) or acquiring (Acquire button) data. Note: For a step-by-step procedure on basic system operation, refer to the "First Light"...
PIXIS-XB System Manual Version 2 Connecting an External Shutter Disconnecting or connecting the shutter cable to the camera while the camera is ON WARNING! can destroy the shutter or the shutter driver in the camera! Introduction While PIXIS-XB cameras do not have an internal shutter, there is provision for...
In WinX and Figure 9. Block Diagram of PIXIS-XB System LightField, the data collection operations use the Experiment Setup parameters to establish the exposure time (the period when signal of interest is allowed to accumulate on the CCD). Focus {Preview} is more likely to be used in setting up the system (see the "First Light"...
The following paragraphs provide step-by-step instructions for verifying the operation of your PIXIS-XB system. The intent of this simple procedure is to help you gain basic familiarity with the operation of your system and to show that it is functioning properly.
Chapter 5 Operation Assumptions The following procedure assumes that 1. You have already set up your system in accordance with the instructions in Chapter 4 and are verifying the camera system operation. 2. You have read the previous sections of this chapter. 3.
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PIXIS-XB System Manual Version 2 Detector Temperature (Setup|Detector Temperature…): Use the default setting. When the array temperature reaches the set temperature, the Detector Temperature dialog will report that the temperature is LOCKED . Note that some overshoot may occur. This could cause temperature lock to be briefly lost and then quickly re-established.
Chapter 5 Operation Figure 11. Example of WinView Data Acquired from First Light Procedure Because the time to acquire and read out an image varies directly with the size of the CCD, the observed frame rate will vary greatly depending on the CCD installed. With a short exposure time, it is not uncommon for the frame readout time to be significantly longer than the exposure time.
PIXIS-XB System Manual Version 2 Getting Started If the system is liquid-cooled, double-check that the CoolCUBE is filled with a 50:50 mixture of ethylene glycol and water and that the hose connections are secure. Refer to “Making the Camera-Circulator Connections for a CoolCUBEII”...
Chapter 5 Operation Figure 13. Experiment Devices Area 3. Note that the Experiment Settings stack on the left now displays several expanders. Because this is a new experiment, the default settings for the camera will be active. 4. Open the Common Acquisition Settings expander and set the Exposure Time to 100 ms.
PIXIS-XB System Manual Version 2 to start Preview mode. In this mode, images 2. Click on the Run button will be continuously acquired and displayed. Since no X-ray source is being used, the acquired images will be of the camera's dark charge.
Chapter 5 Operation Exposure Time Exposure time (set on the Experiment Setup|Main tab { Common Acquisition Settings expander}) is the time between start acquisition and stop acquisition commands sent by the application software to the camera. In combination with triggers, these commands control when continuous cleaning of the CCD stops and when the accumulated signal will be readout.
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Caution If you observe a sudden change in the baseline signal, there may be excessive humidity in the camera vacuum enclosure. Turn off the camera and contact Princeton Instruments Customer Support. See page 92 for contact information. Saturation When signal levels in some part of the image are very high, charge generated in one pixel may exceed the "well capacity"...
Chapter 5 Operation Readout Introduction After the exposure time has elapsed, the charge accumulated in the array pixels needs to be read out of the array, converted from electrons to digital format, and transmitted to the application software where it can be displayed and/or stored.
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PIXIS-XB System Manual Version 2 readout only. Typically the shift register pixels hold twice as much charge as the pixels in the imaging area of the CCD. After the first row is moved into the shift register, the charge now in the shift register is shifted toward the output node, located at one end of the shift register.
Chapter 5 Operation Hardware Binning Hardware binning is performed on the CCD array before the signal is read out of the output amplifier. For signal levels that are readout noise limited this method improves S/N ratio linearly with the number of pixels grouped together.
PIXIS-XB System Manual Version 2 This restriction strongly limits the number of pixels that may be binned in cases where there is a small signal superimposed on a large background, such as signals with a large fluorescence. Ideally, one would like to bin many pixels to increase the S/N ratio of the weak peaks but this cannot be done because the fluorescence would quickly saturate the CCD.
Chapter 5 Operation Output Amplifier {Quality} Selection The output amplifier amplifies the collected charge from the output node and outputs it as electrons/count. Although Figure 18 shows an array with two output nodes and amplifiers (one set at each end of the shift register), some PIXIS-XB systems are available with a single output node and amplifier.
PIXIS-XB System Manual Version 2 1 {Low} requires four electrons to generate one ADU. Strong signals can be acquired without flooding the CCD array. If the gain is set to Low and the images or spectra appear weak, you may want to change the gain setting to Medium or High.
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65535 (16-bit A/D) to a value in the range of 500-600 counts lower. Note: It is important to note that the bias level is not noise. It is a fully subtractable readout pattern. Every device has been thoroughly tested to ensure its compliance with Princeton Instruments' demanding specifications.
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Chapter 6 Advanced Topics Introduction Previous chapters have discussed setting up the hardware and the software for basic operation. This chapter discusses topics associated with experiment synchronization. Synchronization is set up on the Experiment Setup|Timing tab in WinX and on the Trigger and Shutter expanders in LightField.
PIXIS-XB System Manual Version 2 Timing Modes Overview The basic PIXIS-XB timing modes are Free Run {No Response}, External Sync {Readout Per Trigger}, and External Sync {Readout Per Trigger} with Continuous Cleans {Clean Until Trigger}. These timing modes are combined with the Shutter options to provide the widest variety of timing modes for precision experiment synchronization.
Chapter 6 Advanced Topics Free Run {No Response} In the Free Run {No Response} mode the camera does not synchronize with the experiment in any way. The shutter opens as soon as the previous readout is complete, and remains open for the exposure time, t .
PIXIS-XB System Manual Version 2 External synchronization depends on an edge trigger (negative- or positive-going) which must be supplied to the EXT SYNC connector on the back of the camera. The type of edge must be identified in the application software to ensure that the shutter opening is initiated by the correct edge (in WinX, this is done on the Experiment Setup|Timing tab, in LightField on the Trigger/Shutter expander).
Chapter 6 Advanced Topics The PreOpen {Open Before Trigger} mode is useful in cases where an External Sync pulse cannot be provided 8 ms (or the length of time a mechanical shutter takes to open) before the actual signal occurs. Its main drawback is that the CCD is exposed to any ambient light while the shutter is open between frames.
PIXIS-XB System Manual Version 2 Figure 26. Continuous Cleans {Clean Until Trigger} Flowchart Once the External Sync pulse trigger edge is received, cleaning of the array stops as soon as the current row is shifted, and frame collection begins: a delay time of up to one row shift can be expected.
Chapter 6 Advanced Topics Figure 27. WinX Continuous Cleans Timing Diagram Figure 28. LightField Clean Until Trigger (CUT) Timing Diagram EXT SYNC Trigger Input The selected Timing Mode {Trigger Response} determines how the camera will respond to an External Sync pulse that is input at the EXT SYNC connector on the rear of the camera.
PIXIS-XB System Manual Version 2 Fast and Safe Modes Introduction The PIXIS-XB has been designed to allow the greatest possible flexibility when synchronizing data collection with an experiment. The fundamental difference between the Fast and Safe modes is how often the acquisition start and acquisition stop commands are sent by the computer for a data collection sequence.
PIXIS-XB System Manual Version 2 LOGIC OUT Control The TTL-compatible logic level output (0 to +3.3 V) from the LOGIC OUT connector on the rear panel can be used to monitor camera status and control external devices. By default, the logic output level is high while the action is occurring. The timing of the...
Chapter 6 Advanced Topics Kinetics Mode Kinetics Mode is a purchased option for WinX applications. Kinetics Mode is included in the standard LightField application. Introduction Kinetics mode uses the CCD to expose and store a limited number of images in rapid succession.
PIXIS-XB System Manual Version 2 Kinetic Timing Modes Kinetics mode operates with three timing modes: Free Run {No Response}, Single Trigger {Readout Per Trigger}, and Multiple Trigger {Shift Per Trigger}. Free run {No Response} mode is used for experiments that do not require any synchronization with the experiments.
Chapter 6 Advanced Topics Window Size. Integrate signals (SHUTTER {Shutter Open}) or Readout signals (NOT SCAN {Not Reading Out}) are provided at the LOGIC OUT connector for timing measurements. Example: Referring to the readout shown in Figure 32, there are 6 pixels perpendicular to the shift register and the Window Size is 2 pixels high.
PIXIS-XB System Manual Version 2 In multiple trigger mode, the shutter (if there is an external X-ray shutter) is opened when Acquire or Focus {Run} is clicked and each “exposure-shift” cycle in the acquisition is triggered independently by a pulse applied at the EXT SYNC connector. This mode is useful when each subframe needs to be synchronized with a pulsed external light source such as a laser.
Chapter 6 Advanced Topics Software Settings Princeton Instruments does not encourage users to change these parameter settings. For most applications, the default settings will give the best results. We strongly advise contacting the factory for guidance before customizing the chip definition.
PIXIS-XB System Manual Version 2 Custom Timing Notes: 1. This mode is standard with LightField for full frame CCD cameras. 2. This option is fully supported by WinX Version 2.5.18.1 (and higher). In LightField, Custom Timing is accessed via the Custom Sensor button on the Sensor expander.
Chapter 7 Troubleshooting WARNING! Do not attach or remove any cables while the camera system is powered on. Introduction The following issues have corresponding troubleshooting sections in this chapter. Acquisition Started but Viewer Contents Do Not Update Page 64 Baseline Signal Suddenly Changes Page 64 Camera Stops Working Page 64...
If you are sure the problem is in the camera system hardware, begin with these simple checks: Turn off all AC power. Verify that all cables are securely fastened. Turn the system on. If the system still does not respond, contact Princeton Instruments Customer Support.
Chapter 7 Troubleshooting Camera1 (or similar name) in Camera Name field Figure 45. Camera1 in Camera Name Field When the Camera Detection Wizard installs a new camera, the camera is automatically named “Camera#” (where # = 1, 2 , or 3.., depending on the number of cameras detected) This name will appear in the Hardware Setup title bar and as the active camera on the Hardware Setup|Controller/Camera tab.
PIXIS-XB System Manual Version 2 Controller Is Not Responding If this message pops up when you click on OK after selecting the "Interface Type" during Hardware Setup (under the WinView Setup menu), the system has not been able to communicate with the camera. Check to see if camera has been turned ON and if the USB 2.0 interface card, its driver, and the USB cable have been installed.
Chapter 7 Troubleshooting Cooling Troubleshooting Temperature Lock Cannot be Achieved or Maintained. Possible causes for not being able to achieve or maintain lock could include: Ambient temperature greater than +23°C. This condition affects TE-cooled cameras. If ambient is greater than +23°C, you will need to cool the camera environment or raise the set temperature.
PIXIS-XB System Manual Version 2 Gradual Deterioration of Cooling Capability While unlikely with the PIXIS-XB camera (guaranteed permanent vacuum for the life of the camera), if you see a gradual deterioration of the cooling capability, there may be a gradual deterioration of the camera’s vacuum. This can affect temperature performance such that it may be impossible to achieve temperature lock at the lowest temperatures.
Chapter 7 Troubleshooting Data Overrun Has Occurred message Because of memory constraints and the way that USB transfers data, a "Data overrun has occurred" message may be displayed during data acquisition. If this message is displayed, take one or more of the following actions: 1.
PIXIS-XB System Manual Version 2 Cancel the load. Cancelling a load means that the last used experiment will not be loaded automatically when LightField opens. However, you can load the experiment after all the devices are available, you can start a new experiment design, or you can load a different experiment that matches the devices you are using.
Chapter 7 Troubleshooting Program Error message Figure 50. Program Error dialog This dialog may appear if you have tried to acquire a test image, acquire data, or run in focusing mode and the DMA buffer size is too small. A large array (for example, a 2048x2048 array), requires a larger setting than that for a smaller array (for example, a 512x512 array).
PIXIS-XB System Manual Version 2 Serial violations have occurred. Check interface cable. Figure 51. Serial Violations Have Occurred dialog This error message dialog will appear if you try to acquire an image or focus the camera and either (or both) of the following conditions exists: ...
Appendix A Basic Specifications Note: This appendix provides some of the basic specifications of a PIXIS-XB system. If the information you are looking for is not here, it may be available in Appendix B, "Outline Drawings" or on the appropriate data sheet. Data sheets can be downloaded from the Princeton Instruments website (www.princetoninstruments.com).
PIXIS-XB System Manual Version 2 Camera Cooling: Thermoelectric (air) or circulating liquid (CoolCUBE Gain: Software-selectable (high, medium, low) Dimensions: See Appendix B. Connectors: EXT SYNC (MCX): 0 to +3.3 V logic level input to allow data acquisition to be synchronized with external events. Trigger edge can be positive- or negative- going as set in software.
Appendix A Basic Specifications Power (DIN): 12 VDC (6.6A max) input from power supply for small array cameras (PIXIS-XB:400 or PIXIS-XB: 1024). Pin 3, 5: 12 VDC Pin 1, 2, 4: Return Shell: Ground Power (3-pin LEMO): 12 VDC (12.5A max) input from power supply for large array cameras (PIXIS-XB:1300 or PIXIS-XB:2048).
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Appendix B Outline Drawings Notes: 1. Dimensions are in inches [mm]. 2. PIXIS-XB data sheets with the most up-to-date camera outline drawings can be downloaded from the Princeton Instruments website (www.princetoninstruments.com). PIXIS-XB Camera Figure 53. PIXIS-XB:400 and 1024 (Air-Cooled)
Appendix C Cross-Referencing of WinX and LightField Terms WinX-to-LightField WinX LightField Active Rows Parallel to Shift Register Active Height Active Shift Register Columns Active Width ADC Rate Speed ADC Resolution Bit Depth Continuous Cleans Clean Until Trigger Controller Gain Analog Gain Custom Chip Custom Sensor Custom Timing...
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PIXIS-XB System Manual Version 2 WinX LightField Logic Out: Logic 1 Output Signal: Always High Logic Out: Read Out Output Signal: Reading Out Logic Out: Shutter Output Signal: Shutter Open Logic Out: Wait for Trigger Waiting For Trigger Minimum Block Size...
Appendix C Cross-Referencing of WinX and LightField Terms LightField-to-WinX LightField WinX Active Area: Bottom Margin Post-Dummy Rows Parallel to Shift Register Active Area: Left Margin Pre-Dummy Shift Register Columns Active Area: Right Margin Post-Dummy Shift Register Columns Active Area: Top Margin F.T.
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PIXIS-XB System Manual Version 2 LightField WinX Output Signal: Effectively Exposing Logic Out: Expose (Effective) Output Signal: Exposing Logic Out: Expose (Programmed) Output Signal: Reading Out Logic Out: Read Out Output Signal: Shifting Under Mask Logic Out: Image Shift Output Signal: Shutter Open...
(1) year after shipment. During this period, Princeton Instruments will repair the product or, at its sole option, repair or replace any defective part without charge to you. You must deliver the entire product to the Princeton Instruments factory or, at our option, to a factory-authorized service center.
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/ or workmanship under normal use for a period of one (1) year from shipment. Princeton Instruments does not warrant that the function of the software will meet your requirements or that operation will be uninterrupted or error free.
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(1) year limited warranty and/or any other warranty, expressed or implied. 3. All warranty service must be made by the Princeton Instruments factory or, at our option, an authorized service center. 4. Before products or parts can be returned for service you must contact the Princeton Instruments factory and receive a return authorization number (RMA).
Index CCD array blooming ..............38 A/D converters ........... 44, 77 dark charge effects ............. 38 AC power requirements ........... 20 functions performed ........... 36 Acquisition started, viewer contents do not update . 64 maximum on-chip integration ........38 ADC offset............... 45 readout theory ............
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PIXIS-XB System Manual Version 2 Dark current ............. 38 Data loss ..............68 Installation Data Overrun Due to Hardware Conflict message .. 68 PCI drivers............22, 23 Data Overrun Has Occurred message ...... 69 software ..............22, 23 Device is not found ..........69 Interface card Device is Occupied ..........
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Index Output amplifier SHUTTER output level ........56, 76 dual ............... 39, 43 Signal-to-noise ratio High Capacity ............43 on-chip integration ............. 38 Low Noise ..............43 Single Trigger mode ..........59 Software binning............42 Specifications Performance certificate ..........14 operating temperature ..........
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PIXIS-XB System Manual Version 2 Warranties (cont.) owner's manual and troubleshooting ......91 Vacuum deterioration ..........68 sealed chamber ............90 Ventilation requirements ......... 13 software ..............90 Vertical shift rate ............. 62 vacuum integrity ............90 Vignetting ..............72 XP vacuum chamber ..........