Field Diaphragm Of The Epi-Illumination Attachment - Nikon Eclipse 80i Instructions Manual

Field diaphragm of the Epi-illumination attachment

17.4
The field diaphragm controls the illumination on the area of the specimen being viewed.
Operating the field diaphragm lever changes the size of the field diaphragm.
Position of field diaphragm open/close
Pushed in
Pulled
For normal observations, stop down the diaphragm so that the aperture boundaries are just
outside (or inside) the field of view. Illuminating an area broader than necessary will result in
stray light entering the field of view, generating flare, reducing image contrast, and expanding
the area of the specimen subject to fading.
Appropriate field diaphragm settings are particularly important for photomicrography. In general,
good results will be obtained by stopping down the field diaphragm to slightly wider than the
area to be reproduced within the photo frame or monitor display.
Adjust the field diaphragm of the Epi-illumination attachment or DIH-M after performing the
following centering procedures:
Centering the field diaphragm
(1) Adjust the diopter and interpupillary distance
according to the procedures described in
"Epi-fluorescence Microscopy" in the separate
Microscopy manual.
(2) Stop down the field diaphragm. (Pull the field
diaphragm open/close lever.)
(3) Move the center of the field diaphragm image
to the center of the field of view. (Using the
hexagonal screwdriver provided with the
microscope, turn the field diaphragm
centering screws.)
(4) Open up the field diaphragm to match the
field of view. (Push in the field diaphragm
open/close lever.)
(5) Again, move the center of the field diaphragm
image to the center of the field of view. (Turn
the field diaphragm centering screws.)
Individual Operations
17 Fluorescence Observation
lever
27
Field diaphragm setting
Open
Constricted
diaphragm
diaphragm
centering
screw hole
Field diaphragm
open/close lever
Field diaphragm
centering screw hole
Field
image
Field