Epi-Illumination Aperture Diaphragm - Nikon Eclipse 80i Instructions Manual

Epi-illumination Aperture Diaphragm

17.5
The aperture diaphragm determines the numerical aperture for illumination and the optical
system. In Epi-fluorescence microscopy, it is used to adjust image brightness. Use the aperture
diaphragm open/close lever to adjust the size of the opening of the aperture diaphragm.
Position of aperture diaphragm
Pushed in
Pulled
A narrow aperture diaphragm setting reduces the amount of backlighting and makes the image
darker. In contrast, opening up the aperture diaphragm increases backlighting and image
brightness. For ordinary Epi-fluorescence microscopic observations, keep the aperture diaphragm
open. Stop down the diaphragm only when image brightness needs to be adjusted.
Use the aperture diaphragm of the Epi-illumination attachment or DIH-M after performing
centering. (The centering tool may not be included in the set you have purchased.)
Centering the aperture diaphragm
(1) When using the DIH-M, turn on the power switch located on the side of the C-Box.
(2) Close the shutter for the epifluorescent light.
When using the Epi-illumination attachment, set the shutter open/close lever to the "C"
position.
With the DIH-M mounted, the shutter closes automatically when power is switched on. Do
not touch the hand switch. (If the shutter is opened accidentally, press the hand switch
once again to close the shutter.)
(3) Remove one objective from the revolving
nosepiece and insert the centering tool in its
place.
(4) Move the centering tool into the optical path.
(5) Turn on the Epi-fluorescent light source and
open the shutter.
When using the Epi-illumination attachment,
move the shutter open/close lever to the "◯"
position.
When using the DIH-M, press the hand
switch.
Individual Operations
17 Fluorescence Observation
open/close lever
Size of aperture diaphragm
Open
Constricted
Centering
28
Aperture
diaphragm image
tool