Nikon Eclipse 80i Instructions Manual page 8

12.1 Adjusting the aperture diaphragm opening using the condenser scale ................. 22
12.2 Adjusting the aperture diaphragm opening using the centering telescope (optional)22
13 Selecting a Condenser ....................................................................................... 23
14 Adjusting the Field Diaphragm ............................................................................ 23
15 Oil Immersion Operation .................................................................................... 24
16 Water Immersion .............................................................................................. 25
17 Fluorescence Observation................................................................................... 26
17.1 Warning ...................................................................................................... 26
17.2 Epi-illumination attachment shutter ................................................................ 26
17.3 Light shielding plate ..................................................................................... 26
17.4 Field diaphragm of the Epi-illumination attachment .......................................... 27
17.5 Epi-illumination Aperture Diaphragm .............................................................. 28
17.6 Switching Excitation Methods......................................................................... 29
17.7 Epifluorescent ND Filters ............................................................................... 30
18 Selecting Fluorescent Filters ............................................................................... 31
18.1 Selecting excitation filters (EX filters) ............................................................. 31
18.2 Selection of barrier filter (BA filter)................................................................. 32
18.3 Replacing excitation and barrier filters ............................................................ 33
19 Excitation Light Balancer .................................................................................... 34
20 Image Capture ................................................................................................. 36
20.1 Adjusting light intensity ................................................................................ 36
20.2 Adjusting the condenser................................................................................ 36
20.3 Confirming the photomicrographic range ......................................................... 36
20.4 Confirming focus .......................................................................................... 36
20.5 Making adjustments to keep out extraneous light............................................. 36
20.6 Fluorescence photomicrography ..................................................................... 37
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