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Leica Manuals
Microscope
M205 FA
User manual
Leica M205 FA User Manual
Stereo microscope
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Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
General Instructions
Important Safety Notes
Symbols Used
Safety Instructions
Table of Contents
The Leica M Series for Fluorescence
Congratulations
A Step Towards Infinity
The Electronics: Comfort, Convenience and Safety for Your Experiments
The Modular Design: Everything Is Relative
Maximum Compatibility
On We Go
Assembly
Assembly
Assembling the Focusing Column for TL Bases
Assembling the Focusing Column with an Incident-Light Base
Motorized Focus: Restricting the Travel Path
Optics Carrier
Tube
Eyepieces
Objective
UV Protection Screen: Assembly
Objective Nosepiece - Assembly
Objective Nosepiece - Adjusting Parfocality
Leica Fluocombi III - Preparations
Leica Fluocombi III - Assembly
Leica Fluocombi III - Adjusting the Parfocality
Leica Fluocombi III - Centering the Objectives
Leica Fluocombi III - Filter and UV Protection Screen
Transmitted-Light Base Leica TL BFDF: before First Use
Transmitted-Light Base Leica TL BFDF
Leica TL RC / Leica TL RCI
Leica Isopro Manual Mechanical Stage: Assembly
Leica Isopro Motorized Mechanical Stage: Assembly
Cables: Connections
Cables: Cable Duct
Cables: Diagram
Leica LED5000 MCI
Leica LED5000 MCI: Alternative Assembly
Leica LED5000 RL: Assembly
Leica EL6000 - Safety Notes
Leica EL6000 - Preparations
Leica EL6000 - Connection to the Fluorescence Lamp Housing
Leica EL6000 - Replacing the Lamp
Leica EL6000 - Using the Internal Shutter
Quick Start Guide
The Fastest Route to Success
Overview of an M Series Microscope
The Correct Interpupillary Distance
Using the Eyepieces
Focusing
Adjusting the Resistance of the Focus Drive
Changing Magnification (Zoom)
Ratchet Steps and Magnification Levels
Parfocality: more Comfort and Convenience for Your Work
Iris Diaphragm
Eyepieces
Eyepieces
Magnification Factors of the Eyepieces
Health Notes
Dioptric Correction
Dioptric Correction and Parfocality
Graticules
Photography & Video
Photo Tubes and C-Mounts
Trinocular Video/Phototube 50
Leica M Series User Manual
Trinocular Video/Phototube 100
Leica M Series User Manual
Trinocular Video/Phototube 100 %: Ergotube 5
Leica M Series User Manual
Microscope Carrier
Leica M Series User Manual
The Objective Nosepiece
Objectives and Optical Accessories
The Different Types of Objectives
Bases
Leica TL ST Transmitted-Light Base: Controls
Leica TL ST Transmitted-Light Base: Operation
Leica TL ST Transmitted-Light Base: Changing Bulbs
Leica TL BFDF Transmitted-Light Base: Controls
Leica TL BFDF Transmitted-Light Base: Operation
Leica TL RC / Leica TL RCI: Controls
Leica TL RCI: the Deflection Mirror
Leica TL RCI: Color Intensity and Temperature
Leica TL RC / Leica TL RCI: Operation
Leica TL RCI: Methods in Transmitted Light
Leica TL RCI: Relief Images
Using Filters
Leica Isopro (Non-Motorized): Controls
Leica Isopro (Motorized): Controls
System Illumination
Leica LED5000 MCI
Leica LED5000 RL
Leica EL6000 - about the Instrument
Leica EL6000 - Operation
Leica EL6000 - Troubleshooting
Cleaning and Maintenance
Fluorescence
Filter Changer
FIM - Fluorescence Intensity Manager
About Fluorescence Microscopy (Only Applicable for Leica M205 FA)
Rapid Filter Changers and Filter Types
Simple Filter Holders
Equipping the Filter Changer
Observation Without Fluorescence
Double Iris Aperture
Commissioning the Fluorescence System
Accessories
Leica Hand Wheel and Foot Switch
Leica Smartmove
Leica Smarttouch
Leica PSC Controller
Dimensional Drawings
Leica M165 FC
Leica M205 FA
Leica M205 FCA
Leica LED5000 MCI
Leica LED5000 RL
Leica MATS TL
Specifications
Leica M165 FC / Leica M205 FA / Leica M205 FCA
Leica EL6000 - Specifications
Leica TL ST Transmitted-Light Base
Leica TL BFDF Transmitted-Light Base
Leica TL RC / Leica TL RCI
Leica TL5000 Ergo
Leica Isopro Motorized XY Stage
Appendix
Calculating the Total Magnification and Field of View Diameter
Care, Maintenance, Contact Persons
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Quick Links
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General Instructions
2
Leica El6000 - Replacing the Lamp
3
Leica El6000 - about the Instrument
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User Manual
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Summarization of Contents
General Instructions
Safety concept
Information about handling and care of the instrument.
Use in clean rooms
Guidelines for using the Leica M series in clean rooms.
Cleaning
Instructions for cleaning microscope components.
Servicing
Information on authorized repairs and spare parts.
Responsibilities of person in charge of instrument
Ensuring proper operation and maintenance by authorized personnel.
Important Safety Notes
User manual
Information on accessing and using user manuals.
Special manuals
List of manuals provided for specific accessories.
Symbols Used
Warning of a danger
Indicates important information that must be read and complied with.
Warning of hazardous electrical voltage
Highlights mandatory safety information regarding electrical voltage.
Danger due to hot surface
Warns against touching accessible hot surfaces.
Important information
Provides additional information or explanations.
Safety Instructions
Description
Overview of modules and their requirements.
Intended use
Guidelines for proper usage of the microscope.
Non-intended use
Warning against improper usage of the microscope.
Place of use
Environmental and placement guidelines for the instrument.
Responsibilities of person in charge of instrument
Ensuring safe operation and maintenance by authorized personnel.
Safety Instructions (Continued)
Repairs, service work
Information on repairs, service work and spare parts.
Transport
Guidelines for shipping and moving microscope components.
Health risks
Potential health issues associated with microscope use.
Safety Instructions (Continued)
Light sources: Safety regulations
Protective measures related to light sources.
Warning
Warning about potential eye damage from UV radiation.
Safety Instructions (Continued)
Supply unit
Safety precautions for the power supply unit.
Lamp housing
Safety precautions for the lamp housing.
Mercury lamp
Safety and handling instructions for mercury lamps.
The Leica M Series for Fluorescence
Congratulations!
Welcome message and overview of the Leica M series.
A Step Towards Infinity
Discusses optical advancements and resolution limits.
The Electronics: Comfort, Convenience and Safety for Your Experiments
Details on electronic integration and its advantages.
The Modular Design: Everything is Relative
Explains the flexibility and adaptability of the modular design.
Maximum Compatibility
Discusses compatibility with existing series and components.
On We Go
Guidance on proceeding with setup or quick start.
Assembly
Assembling the Focusing Column for TL Bases
Step-by-step guide for attaching the focusing column.
Assembling the Focusing Column With an Incident-light Base
Guide for assembling the column on an incident-light base.
Motorized Focus: Restricting the Travel Path
Procedure to limit the travel path of the motorized focus.
Optics Carrier
Instructions for assembling the optics carrier.
Tube
Instructions for assembling the microscope tube.
Eyepieces
Instructions for inserting and securing eyepieces.
Objective
Instructions for attaching the objective.
UV Protection Screen: Assembly
Steps for mounting the UV protection screen.
Objective Nosepiece – Assembly
Guide for assembling the objective nosepiece.
Objective Nosepiece – Adjusting Parfocality
Procedure for setting objective parfocality.
Leica FluoCombi III
Preparations
Steps to perform before assembling the FluoCombi III.
Assembly
Steps for assembling the FluoCombi III.
Assembly (Continued)
Further steps for assembling the FluoCombi III.
Adjusting the Parfocality
Procedure for adjusting parfocality for the FluoCombi III.
Adjusting the Parfocality (Continued)
Continuation of the parfocality adjustment procedure.
Centering the Objectives
Steps for centering the objectives.
Filter and UV Protection Screen
Installing the filter and UV protection screen.
Transmitted-light Base Leica TL BFDF
Before First Use
Removing transport anchors before initial use.
Standard delivery
Details on the standard base delivery.
Stage assembly
How to equip the base with different stages.
Standard stage
Steps to mount the standard stage onto the base.
Leica TL RC / Leica TL RCI
Standard delivery
Details on the standard base delivery.
Stage assembly
How to equip the base with different stages.
Leica IsoPro Manual Mechanical Stage: Assembly
Leica IsoPro Mechanical Stage
Mounting instructions for the Leica IsoPro mechanical stage.
Left or right operation
Configuration for control buttons on the left or right side.
Assembly (Continued)
Continuation of the mechanical stage assembly process.
Leica IsoPro Motorized Mechanical Stage: Assembly
Basics
Overview and compatibility of the motorized stage.
Mechanical stage and base
Steps to mount the motorized stage onto the base.
Assembly (Continued)
Continuation of the motorized stage assembly process.
Cables: Connections
The terminals
Description of connection terminals on the column.
Cables: Cable Duct
Feeding the cables
Procedure for routing and stowing cables in the cable duct.
Leica LED5000 MCI
Assembly
Instructions for installing the LED5000 MCI illuminator.
Constraints
Limitations on using the MCI with the objective nosepiece.
Alternative Assembly
Alternative mounting method for the LED5000 MCI.
Leica LED5000 RL: Assembly
Required tools
List of tools required for assembling the LED5000 RL.
Assembly
Steps for assembling the LED5000 RL illuminator.
Constraints
Usage limitations for the LED5000 RL illuminator.
Assembly (Continued)
Further details for assembling the LED5000 RL.
Leica EL6000
Safety Notes
General safety notes and standards for the EL6000.
Safety Notes (Continued)
Continued safety precautions for the EL6000.
Safety Notes (Continued)
Electrical safety and environmental considerations.
Preparations
Instrument setup and preparation for lamp insertion.
Preparations (Continued)
Continuation of lamp insertion steps.
Preparations (Continued)
Final steps for lamp insertion and securing.
Connection to the Fluorescence Lamp Housing
Connecting the light guide to the fluorescence lamp housing.
Connection to the Fluorescence Lamp Housing (Continued)
Final connection steps for the light guide.
Replacing the Lamp
Procedure for replacing the lamp, including safety precautions.
Operation
Using the internal shutter and other controls.
Troubleshooting
Common faults and remedies for the EL6000.
Cleaning and Maintenance
Procedures for cleaning and maintaining the EL6000.
Quick Start Guide
The Fastest Route to Success
Introduction to the Quick Start Guide.
Overview of an M Series Microscope
Identifies key components of the microscope.
The Correct Interpupillary Distance
Guide to setting the interpupillary distance.
Using the Eyepieces
Instructions on how to use eyepieces, with/without glasses.
Focusing
Explanation of focusing mechanisms and adjustments.
Adjusting the Resistance of the Focus Drive
How to adjust the focus drive resistance.
Changing Magnification (Zoom)
Instructions for changing magnification.
Ratchet Steps and Magnification Levels
Explanation of ratchet step functionality.
Parfocality: More Comfort and Convenience for Your Work
Explanation of parfocality and its benefits.
Iris Diaphragm
How the iris diaphragm affects light and depth of field.
Eyepieces
Magnification Factors of the Eyepieces
Table of eyepiece magnifications and order numbers.
Health Notes
Potential sources of infection from eyepieces.
Dioptric Correction
How to use the built-in dioptric correction.
Dioptric Correction and Parfocality
Adjusting diopters and maintaining parfocality.
Graticules
Using graticules for measurements and counting.
Photography & Video
Photography & Video
Importance of digital documentation and Leica cameras.
Leica DFC cameras
Information on digital Leica DFC cameras.
Leica Application Suite
Overview of LAS software for microscope control.
Photo Tubes and C-mounts
Details on connecting cameras via C-mount adapters.
Trinocular Video/Phototube 50 %
Use and assembly of the 50% trinocular tube.
Trinocular Video/Phototube 100 %
Use and switchover of the 100% trinocular tube.
Trinocular Video/Phototube 100 %: ErgoTube 5°– 45°
Ergonomics and function of the ErgoTube.
Microscope Carrier
The Objective Nosepiece
Functionality and technical constraints of the objective nosepiece.
Objectives and Optical Accessories
The Different Types of Objectives
Overview of achromatic and planapochromatic objectives.
Bases
Leica TL ST Transmitted-light Base: Controls
Identification of controls on the TL ST base.
Leica TL ST Transmitted-light Base: Operation
Operating the TL ST base for illumination.
Leica TL ST Transmitted-light Base: Changing Bulbs
Procedure for changing the halogen lamp.
Leica TL BFDF Transmitted-light Base: Controls
Identification of controls on the TL BFDF base.
Leica TL BFDF Transmitted-light Base: Operation
Operating the TL BFDF base for bright/dark field illumination.
Leica TL RC / Leica TL RCI: Controls
Identification of controls on TL RC and TL RCI bases.
Leica TL RCI: The Deflection Mirror
Functionality and adjustment of the deflection mirror.
Leica TL RCI: Color Intensity and Temperature
Controlling color intensity and temperature via mouse.
Leica TL RC / Leica TL RCI: Operation
Operating relief contrast settings.
Leica TL RCI: Methods in Transmitted Light
Using transmitted light techniques like bright/dark field.
Leica TL RCI: Relief Images
Setting up positive and negative relief contrast.
Using Filters
How to insert and use filters in the filter holder.
Leica IsoPro (Non-motorized): Controls
Operating the manual IsoPro stage.
Leica IsoPro (Motorized): Controls
Controls for motorized IsoPro stage and modules.
System Illumination
Leica LED5000 MCI
Use and preparation of the MCI illuminator.
Leica LED5000 MCI (Continued)
Keyboard and illumination scenario controls for MCI.
Leica LED5000 RL: Assembly
Steps for assembling the LED5000 RL illuminator.
Leica LED5000 RL: Assembly (Continued)
Further details for assembling the LED5000 RL.
Leica EL6000
General information and safety notes for the EL6000.
Leica EL6000 – Replacing the Lamp
Procedure for replacing the lamp, including safety precautions.
Leica EL6000 – Operation
Using the internal shutter and other controls.
Leica EL6000 – Troubleshooting
Common faults and remedies for the EL6000.
Cleaning and Maintenance
Procedures for cleaning and maintaining the EL6000.
Fluorescence
Filter Changer
System for filter management and safety notes.
FIM – Fluorescence Intensity Manager
Managing fluorescence intensity levels.
About Fluorescence Microscopy (Only Applicable for Leica M205 FA)
Principles and benefits of fluorescence microscopy.
Rapid Filter Changers and Filter Types
List of available filter sets for the rapid changer.
Simple Filter Holders
Use and safety notes for simple filter holders.
Equipping the Filter Changer
Steps for inserting filters into the filter changer.
Observation Without Fluorescence
How to view the specimen without fluorescence illumination.
Double Iris Aperture
How the double iris aperture affects depth of field.
Commissioning the Fluorescence System
Steps for setting up and commissioning the fluorescence system.
Accessories
Leica Hand Wheel and Foot Switch
Functionality of hand wheel and programmable foot switches.
Leica SmartMove
Control unit for handling motorized Leica instruments.
Leica SmartTouch
Touchscreen control unit for experiment procedures.
Leica PSC Controller
Precision control for Leica IsoPro motorized stages.
Dimensional Drawings
Leica M165 FC
Dimensional drawings of the Leica M165 FC.
Leica M165 FC (Continued)
Additional dimensional drawings for Leica M165 FC.
Leica M165 FC (Continued)
More dimensional drawings for Leica M165 FC.
Leica M205 FA
Dimensional drawings of the Leica M205 FA.
Leica M205 FA (Continued)
Additional dimensional drawings for Leica M205 FA.
Leica M205 FA (Continued)
More dimensional drawings for Leica M205 FA.
Leica M205 FCA
Dimensional drawings of the Leica M205 FCA.
Leica M205 FCA (Continued)
Additional dimensional drawings for Leica M205 FCA.
Leica M205 FCA (Continued)
More dimensional drawings for Leica M205 FCA.
Leica LED5000 MCI
Dimensional drawings of the Leica LED5000 MCI.
Leica LED5000 RL
Dimensional drawings of the Leica LED5000 RL.
Leica MATS TL
Dimensional drawings of the Leica MATS TL.
Specifications
Leica M165 FC / Leica M205 FA / Leica M205 FCA
Optical data and maximum values for the microscope models.
Leica M165 FC / Leica M205 FA / Leica M205 FCA (Continued)
Optics carrier and accessory details for the models.
Leica M165 FC / Leica M205 FA / Leica M205 FCA (Continued)
Additional specifications for microscope models.
Leica M165 FC / Leica M205 FA / Leica M205 FCA (Continued)
Module system overview and accessories.
Leica EL6000 – Specifications
Technical specifications for the EL6000 light source.
Leica EL6000 – Specifications (Continued)
Light output, interface, and protective functions.
Leica TL ST Transmitted-light Base
Specifications for the TL ST transmitted-light base.
Leica TL BFDF Transmitted-light Base
Specifications for the TL BFDF transmitted-light base.
Leica TL RC / Leica TL RCI
Specifications for TL RC and TL RCI bases.
Leica TL5000 Ergo
Specifications for the TL5000 Ergo.
Leica IsoPro Motorized XY Stage
Technical specifications for the motorized XY stage.
Appendix
Calculating the Total Magnification and Field of View Diameter
Formulas for calculating magnification and field of view.
Care, Maintenance, Contact Persons
Guidelines for microscope care, maintenance, and contacts.
Care, Maintenance, Contact Persons (Continued)
Further advice on protection from dirt and cleaning.
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