Shimadzu UV-1900i Instruction Manual
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Shimadzu UV-1900i Instruction Manual

Shimadzu UV-1900i Instruction Manual

Shimadzu spectrophotometer
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Shimadzu Spectrophotometer
UV-1900i
Instruction Manual
Operation Guide
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
207-90563
Sep. 2019

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Summary of Contents for Shimadzu UV-1900i

  • Page 1 207-90563 Sep. 2019 Shimadzu Spectrophotometer UV-1900i Instruction Manual Operation Guide Read this manual thoroughly before you use the product. Keep this manual for future reference.
  • Page 2 This page is intentionally left blank.
  • Page 3 • To ensure safe operation, read all "Safety Instructions" P.iv thoroughly before using the product. • To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, re-installation (after the product is moved), or repair is required. Notice • Information in this manual is subject to change without notice and does not represent a commitment on the part of the vendor.
  • Page 4 Describes installation and maintenance of (Installation and Maintenance S207-90555 the UV-1900i. Guide) UV-1900i Basic Operation S207-90571 Describes basic operation of the UV-1900i. Guide UV-1900i Important Describes the instructions for using the S207-90578 Information – Please Read UV-1900i safely. UV-1900i Operation Guide...
  • Page 5 The following symbols are used in this manual: Indication Meaning Indicates an action that must not be performed. Indicates an action that must be performed. Indicates the location of related reference information. UV-1900i Operation Guide...

  • Page 6: Introduction

    If the installation site does not have sufficient strength or stability, the instrument may tip over or fall down, causing an accident. Do NOT install the UV-1900i in an area with corrosive gases or a dusty place. It may cause decline in performance or shorten the life.
  • Page 7 Install the product in an indoor location under the following classifications: installation category II, pollution level 2, and altitude 2,000 meters max. n Installation To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, or re-installation (after the product is moved) is required.
  • Page 8 Introduction The following table shows power supply voltage and power consumption of the UV-1900i. Be sure to connect the power supply that meets the indicated specifications. Failure to do so may result in fire, electric shock, and instrument malfunction. Unstable power supply voltage or insufficient power capacity may deteriorate the performance.
  • Page 9 If the sample is spilled, dispose of it according to handling and disposal methods described in the SDS (Safety Data Sheet) of the sample. When using electronic equipment with the UV-1900i, be careful not to spill liquid (sample, etc.) on the equipment.
  • Page 10 Other parts may cause damage of parts or malfunction and prevent proper operation. Do NOT leave any spilled water on the UV-1900i. Do NOT use alcohol or paint thinner solvents for cleaning. If water or a solvent is left on the instrument, it may cause rust or discoloration.
  • Page 11 In case of electrical failure, perform the following operations. Press the "○" side of the power switch located on the right side bottom of the UV-1900i to turn OFF the instrument. After the power comes back on, start up the UV-1900i normally by following "Installation" P.v "Operation"...
  • Page 12 In order to ensure safety, warning labels are attached in places requiring caution. If a warning label is lost or damaged, obtain a new label through your Shimadzu representative and attach it in the correct position. See "Warning Labels on the Equipment"...
  • Page 13 "List of Residual Risks". n Residual Risk Map The "Mechanical Location" and "No." indicated below are in accordance with those in "List of Residual Risks". For details, see "List of Residual Risks". UV-1900i Operation Guide...
  • Page 14 Maintenance overheats the to prevent it from Guide)" lamp may being soiled by Operation damage the fingerprints, etc. Lamp lighting Category instrument or explode. Qualified person Required received training Qualification/ to use the Education instrument UV-1900i Operation Guide...
  • Page 15 Be careful not to Guide)" handling during bump the lamp replacement, Lamp Operation against other may result in Category replacement objects. injury. Qualified person Required received training Qualification/ to control the Education instrument xiii UV-1900i Operation Guide...
  • Page 16 No specific Falling or support the total location dropping may weight of the Required cause an injury. instrument including Instrument Qualification/ peripherals and has manager Edcation a flat and stable surface with at least 600 mm depth. UV-1900i Operation Guide...

  • Page 17: Warranty

    Shimadzu may not be able to provide identical replacement products. 3. Limitation of Liability: (1) In no event will Shimadzu be liable for any lost revenue, profit or data, or for special, indirect, consequential, incidental or punitive damages, however caused regardless of the theory of liability, arising out of or related to the use of or inability to use the product, even if Shimadzu has been advised of the possibility of such damage.

  • Page 18: After-Sales Service And Availability Of Replacement Parts

    Replacement parts for this product will be available for a period of seven (7) years after the product is discontinued. Thereafter, such parts may cease to be available. Note, however, that the availability of parts not manufactured by Shimadzu shall be determined by the relevant manufacturers.

  • Page 19: For California, Usa Only

    See www.dtsc.ca.gov/hazardouswaste/perchlorate Electromagnetic Compatibility Descriptions in this section apply only to the following models: • 207-25700-58 UV-1900i This product complies with European standard EN61326, class B for electromagnetic interference (Emissions) and industrial immunity test requirements (Immunity). This product complies with the basic immunity test requirements (Immunity) only when used with the optional instruments CPS-100 and TCC-100.

  • Page 20: Table Of Contents

    Login and Logout ................2.2.1 Changing User Level and Login ............2.2.2 Logout Using USB Memory ..........Using Expanded Memory ........2.4.1 Acquisition of Files in the Expanded Memory by the PC Processing for Measurement Data ....... xviii UV-1900i Operation Guide...
  • Page 21 Transfer of Measurement Data to the PC ... Managing Files (Copy, Delete, Display and Operation of Files ......Save in a CSV Format) Photometric Setting Measurement Parameters ......(One-Wavelength) Measurement ..................6.2.1 Performing Measurement ....... 6.2.2 Checking Measurement Results UV-1900i Operation Guide...
  • Page 22 Setting Attachments ........Creating a Calibration Curve ........ 9.4.1 When Absorbance of the Standard Sample is Known 9.4.2 When Absorbance of the Standard Sample is Unknown Saving and Loading Parameters Files in the Quantitation Mode .......... Measurement ..........UV-1900i Operation Guide...
  • Page 23 ........11.2.1 Performing Measurement ......11.2.2 Checking Measurement Results Printing Measurement Results ......11.3 ........... 11.3.1 Auto Print ........11.3.2 Manual Print (Table Data) ........11.3.3 Manual Print (Curve data) ......11.3.4 Manual Print (Curve Total Plot) UV-1900i Operation Guide...
  • Page 24 14.3 ......14.3.1 Performing Arithmetic Operations ....14.3.2 Printing the Results (Arithmetic Operations) Derivative ............14.4 ........14.4.1 Data Processing Method ......14.4.2 Performing Derivative Processing ......14.4.3 Printing the Results (Derivative) Peak Pick ............14.5 xxii UV-1900i Operation Guide...
  • Page 25 15.13.3 Setting the Printer Name (Only When Selecting ........... PictBridge) 15.13.4 Changing the Paper Size (Only When Selecting ........... PictBridge) Setting the Keyboard Layout ......15.14 Setting the Expanded Memory ......15.15 Setting the Start Program ........ 15.16 xxiii UV-1900i Operation Guide...
  • Page 26 17.4.10 Setting - [EP] Limit of Stray Light ......17.4.11 Setting - [USP] Limit of Stray Light ........17.4.12 Setting - [EP] Resolution ........17.4.13 Setting - [USP] Resolution ....17.4.14 Setting - [JP] Wavelength Accuracy (D2) xxiv UV-1900i Operation Guide...
  • Page 27 17.7.17 Operation During the Test - Resolution (Spectral ........... Bandwidth) ....17.7.18 Operation During the Test - Noise Level ..... 17.7.19 Operation During the Test - Baseline Flatness ..17.7.20 Operation During the Test - Drift (Baseline Stability) UV-1900i Operation Guide...
  • Page 28 17.9.3 Loading Test Results File ........17.9.4 Save Test Results as CSV ........... 17.9.5 Executed Test List Printing Test Results ........17.10 ......... 17.10.1 Test Data Print (Each Test) ........17.10.2 Test Data Print (All Data) xxvi UV-1900i Operation Guide...
  • Page 29 (Common to the Sipper 160 and Syringe Sipper) .................. 19.5.1 Operation of [Auto Meas.] or [Rinse] ....19.5.2 Operation of [Auto Zero] and [Base Corr.] ..19.5.3 Notes for Interlocking ASC-5 in Quantitation Mode Index ............xxvii UV-1900i Operation Guide...
  • Page 30 This page is intentionally left blank.

  • Page 31: Overview Of The Instrument

    • To put it back, push it until it clicks. 1.1.2 Operation Using a Personal Computer (PC) Set the instrument to the external control mode to control it using a PC. "18 PC Control Mode" P.427 UV-1900i Operation Guide...

  • Page 32: Connection To The Instrument And Necessary Devices

    1 Overview of the Instrument Connection to the Instrument and Necessary Devices For typical systems that use UV-1900i, this section outlines how to connect them and available functions. 1.2.1 Operation on the Touch Panel Screen (without Connection to the Network) The connection method is as shown below.
  • Page 33 Connector for memory is Saving/copying data in the Expanded the expanded performed by expanded memory (optional) memory memory (built-in) our authorized service personnel. PictBridge is a registered trademark of the Camera & Imaging Products Association. UV-1900i Operation Guide...

  • Page 34: Operation On The Touch Panel Screen (With Connection To The Network)

    LAN measurement). function, a LAN cable is PC for data required to Acquisition of files in the expanded acquisition connect to the memory by the PC with FTP client Expanded network. software memory LAN cable UV-1900i Operation Guide...
  • Page 35 Saving/copying data in a USB USB memory memory (optional) The installation of the expanded Connector for memory is Saving/copying data in the Expanded the expanded performed by expanded memory (optional) memory memory (built-in) our authorized service personnel. UV-1900i Operation Guide...

  • Page 36: Operation Using A Personal Computer

    LabSolutions UV-Vis communicate with the PC for control USB connector instrument at Control by optional software USB cable (For PC control) the same time. (UVProbe, UV Performance • Communication Validation, etc.) via LAN cable is not possible. UV-1900i Operation Guide...

  • Page 37: Before Operating

    Before turning "ON" the power switch, make sure that nothing is placed in the sample compartment and the cell holder. If the power is turned "ON" when a sample cell is mounted into the UV-1900i, Light Source Energy Check and WL Check function may detect it as an error since the light beam is obstructed.

  • Page 38: Turning On Power And Initialization

    "Baseline Correction" ([Base Corr.] in the buttons of the instrument). 2.1.2 Turning ON Power and Initialization When the instrument power is turned ON, the UV-1900i starts executing various checks and initializations. For details on this procedure, refer to "2.5 Turning ON/OFF the Power and Initialization"...

  • Page 39: Login And Logout

    2.2 Login and Logout Login and Logout The UV-1900i has a security function that limits function availability according to user level. When the security function is enabled, login is required after completing initialization at power-on and logging out of the current user level.
  • Page 40 2 Before Operating Enter the password. Tap the [Password] entry field. Enter the password. Text Input Screen ("4.2.1 Text Input Screen (Keyboard)" P.27) Tap [Login]. After login, the [Mode Menu] screen ("3.1 Mode Menu Screen" P.18) appears. UV-1900i Operation Guide...

  • Page 41: Logout

    2.2 Login and Logout 2.2.2 Logout Tap [Log Out] while logged in. Logout is completed and the login screen appears "2.2.1 Changing User Level and Login" P.9. UV-1900i Operation Guide...

  • Page 42: Using Usb Memory

    P.32. • Read measurement data file on a spreadsheet software. The UV-1900i can convert the measurement data file to a CSV format file, which can be read on any software supporting the format. For details on CSV conversion of the measurement data file, see "5 Managing Files...
  • Page 43 • Number of files which can be saved in the USB memory depends on its capacity. • UV-1900i uses 3 file types: Parameters File, Curve Data File, and Table Data File. The instrument can display limited number of files in the USB memory.

  • Page 44: Using Expanded Memory

    • Number of files which can be saved in the expanded memory depends on its capacity. • UV-1900i uses 3 file types: Measurement Parameters File, Curve Data File, and Table Data File. The instrument can display limited number of files in the expanded memory.

  • Page 45: Acquisition Of Files In The Expanded Memory By The Pc

    Turn ON the power of the PC. Enter "ftp://UV-1900@xxx.xxx.xxx.xxx (IP address of the instrument)" in the address bar of the Explorer and go there. "15.6 Network Settings" P.245 The password entry screen appears. Enter "Shimadzu" and click [Log On]. UV-1900i Operation Guide...
  • Page 46 Open the folder named [UV-1900] that appears on the Explorer. Folders containing various data are displayed on the Explorer. Various data can be copied to the PC. The storage destination of the data is shown in "4.3.3 Destination Folder When Using a USB Memory/Expanded Memory" P.33. UV-1900i Operation Guide...

  • Page 47: Processing For Measurement Data

    2.5 Processing for Measurement Data Processing for Measurement Data When performing measurement, the UV-1900i normally obtains data containing more decimal places than those displayed on the screen. For curve data, obtained actual data is saved. For table data, the instrument processes photometric value as follows, calculate concentrations, etc.

  • Page 48: Selecting Measurement Mode And Function Menu

    ("16.4 Security Mode" P.274) is enabled, the login screen is displayed after completing initialization. For the login procedure, see "2.2.1 Changing User Level and Login" P.9. Name Description Displays the status of UV-1900i. Status Bar "Status Bar" P.20 UV-1900i Operation Guide...
  • Page 49 "5 Managing Files (Copy, Delete, Save in a CSV Format)" P.48 • [Maintenance] Function Menu Displays the screen for maintenance of the UV-1900i (e.g. validation and usage time check for the light source lamp), as well as security function settings. "16 Maintenance" P.269 •...
  • Page 50 Displays the status of parts of the instrument, date and time. Name Description Instrument Displays the status of UV-1900i. Status [-] is displayed when not using optional 6-position Multi-Cell and MMC-1600, while the cell number is displayed when using Multi-Cell Status them.

  • Page 51: Overview Of Measurement Mode And Function Menu

    Single beam energy measurement can also be performed. Data processing such as peak pick, smoothing, and arithmetic operations may be applied to the measured spectrum. "8 Spectrum" UV-1900i Operation Guide...
  • Page 52 Up to 16 samples can be measured at once using a multi-cell holder (optional). Data processing such as arithmetic operations may be applied to the photometric values. "12 Time Course" UV-1900i Operation Guide...

  • Page 53: Overview Of Function Menu

    Parameters File/Measurement Data File Display Function Displays stored files. You can copy or delete them, or save them in CSV format (only table data file and curve data file). "5 Managing Files (Copy, Delete, Save in a CSV Format)" P.48 UV-1900i Operation Guide...
  • Page 54 • Security Settings <Update System Program> • Update System Program "16 Maintenance" n PC Control Function This mode allows the UV-1900i to be controlled by an external computer. Select this mode when using the "LabSolutions UV-Vis" software included with this instrument.

  • Page 55: Common Operations On The Measurement Mode Screen

    Saves set measurement parameters and results as a file. The button name varies according to the tab. Save Files button "4.3 Saving Files" P.32 Loads set measurement parameters and results. The button Load Files name varies according to the tab. button "4.4 Loading Files" P.37 UV-1900i Operation Guide...
  • Page 56 Name Description Page Switching When selectable items continue for more than one page, tap button this button to switch displayed page. [Cancel] Closes the screen canceling the change. Selectable items The list of selectable items. UV-1900i Operation Guide...

  • Page 57: Entering Text And Numbers

    (keyboard) and numeric input screen where you can enter only numbers (10-button keypad) are available. 4.2.1 Text Input Screen (Keyboard) When tapping an input field such as Sample Name, text input screen (keyboard) is displayed. Alphanumerics Symbols UV-1900i Operation Guide...
  • Page 58 Switches between uppercase and lowercase characters. button Space button Enters a space. [Apply] Confirms entered text and closes the window. Cursor-control Moves the cursor in the display field to the left or right. button [Cancel] Discards entered text and closes the window. UV-1900i Operation Guide...

  • Page 59: Numeric Input Screen (10-Button Keypad)

    Input button [.] (decimal point) and [-] (minus) are not available for some fields. [Apply] Confirms entered number and closes the window. Backspace Deletes the number before the cursor. button [Cancel] Discards entered number and closes the window. UV-1900i Operation Guide...

  • Page 60: Input Using An External Keyboard/Barcode Reader

    ", |, *, : , <, >, ? , . , /, Photometric (One-Wavelength) [Photo. Table] tab [Sample Name] can be edited. Photometric 8λ (Multi-Wavelength) Quantitation [Unk. Table] tab [Sample Name] can be edited. Bio Method UV-1900i Operation Guide...
  • Page 61 Upper-case alphabetic characters are displayed when CapsLock is enabled. • [Shift] + [Alphabet] button: Upper-case alphabetic characters Lower-case alphabetic characters are displayed when CapsLock is enabled. • [Numbers] button: Numbers You cannot enter numbers when NumLock is enabled. UV-1900i Operation Guide...

  • Page 62: Saving Files

    • Time Course • Photometric • Photometric 8l • Quantitation Table Data File • Kinetics • Kinetics Rate • Bio Method Validation Parameters File Validation Validation Results File The maximum number of files you can save in built-in memory. UV-1900i Operation Guide...

  • Page 63: Extensions Of Files

    4.3.3 Destination Folder When Using a USB Memory/Expanded Memory When a USB memory or expanded memory is specified as the destination, files are saved in the following folders. If the destination folder does not exist, the UV-1900i creates it automatically.

  • Page 64: Saving Files

    • Repeated measurement in several spectrum modes (when a USB memory or expanded memory is connected and Auto File is performed ("8.2.3 Auto File Function")) • Measurement using multiple cells in the Kinetics/Time Course mode button. UV-1900i Operation Guide...
  • Page 65 When the file cannot be saved because capacity of the destination location is insufficient, a message appears. Delete unnecessary files according to "When [The built-in memory (or USB memory, expanded memory) capacity is low..] is displayed" P.36 or connect a USB memory with sufficient free space. UV-1900i Operation Guide...
  • Page 66 • Connect a USB memory to the PC and delete unnecessary files. • Connect a USB memory with sufficient free space. • Format the expanded memory. "15.15 Setting the Expanded Memory" P.261 If you perform formatting, all saved files will be deleted. UV-1900i Operation Guide...

  • Page 67: Loading Files

    The instrument can display limited number of files in the USB memory and the expanded memory. 999 files can be displayed for each file type. Files are displayed in alphabetical order. button. Specify the location of the file to be loaded. UV-1900i Operation Guide...
  • Page 68 Moves to the previous or next page when the list continues for more than one page. • Selects the previous or next file. [Open] Loads the selected file. Graph Display Displays the graph. This function is available when tapping button [Load Curve] to load Curve Data File. UV-1900i Operation Guide...
  • Page 69 Tap this button to enable the function (highlighted in blue) to Multiple select multiple files. Selection button Tap a selected file to cancel the selection when multiple files are selected. [Close] Cancels the file loading and returns to the previous screen. UV-1900i Operation Guide...

  • Page 70: Print/Hard Copy/Screen Shot

    4 Common Operations Print/Hard Copy/Screen Shot Connect optional or commercially available printer to the UV-1900i to print measurement results. You can save displayed screen as an image file by connecting a USB memory. 4.5.1 Data Print Prints measurement results as a numerical data table and graph.
  • Page 71 "11.3.2 Manual Print (Table Data)" Measured data - (point where data is P.154 Curve total plot obtained) of • "11.3.3 Manual Print (Curve data)" measurement results in a P.155 numerical data table. • "11.3.4 Manual Print (Curve Total Plot)" P.157 UV-1900i Operation Guide...
  • Page 72 "14.6.3 Printing the Results (Point Pick)" P.225 • "14.7.3 Printing the Results (Peak Area)" P.231 • "17.10.1 Test Data Print (Each Test)" P.404 All test data/ Prints results of validation. Test data • "17.10.2 Test Data Print (All Data)" P.405 UV-1900i Operation Guide...

  • Page 73: Hard Copy

    4.5.2 Hard Copy Prints displayed screen by the printer. This function is available only when PictBridge printer is connected. Tap [Print] on the screen to be printed. Tap [Hard copy]. Displayed screen is printed by the printer. UV-1900i Operation Guide...

  • Page 74: Screen Shot (Saving An Image)

    Displayed screen is saved as an image file. To save an error message as an image file, tap [Screen Shot] shown in the message. If [Screen Shot] is not displayed, connect a USB memory to display it. UV-1900i Operation Guide...

  • Page 75: Transfer Of Measurement Data To The Pc

    Data is automatically transferred each time measurement is performed. The output format is the print format for auto printing of Photometric (one-wavelength measurement) mode plus the KFactor column. "6.3.1 Auto Print" P.60 Measurement conditions cannot be output. UV-1900i Operation Guide...
  • Page 76 • If [Repetitions] is set to two or more, only the last measurement data can be transferred. • When multiple cells are measured using the multiple cell holder, only the last measured cell data can be transferred. UV-1900i Operation Guide...
  • Page 77 4.6 Transfer of Measurement Data to the PC UV-1900i Operation Guide...

  • Page 78: Managing Files

    Tap [Data File] to display curve data file or table data file. Tap the saving destination of the file to be loaded. You can select [Built-in Memory], [USB Memory], or [Expanded Memory]. The expanded memory is an optional item. UV-1900i Operation Guide...

  • Page 79: Photometric (One-Wavelength)

    • Quantitation • Biuret Method • Kinetics • Kinetics Rate • UV Method Graph Display Displays the graph. This button is enabled when curve data file button is selected. Preview button Displays the details of the selected file. UV-1900i Operation Guide...
  • Page 80 For details on CSV format files, see "To save CSV format files" Saving in CSV P.51. Format button When parameters file is displayed, this button is disabled. Delete button Deletes the selected file. [Open] Displays the file selected in the file list. UV-1900i Operation Guide...
  • Page 81 5.1 Display and Operation of Files n To save CSV format files Curve data or table data file obtained by the UV-1900i can be converted to a CSV (Comma Separated Values) format text file and saved in a USB memory.

  • Page 82: Setting Measurement Parameters

    The measurement screen is displayed. Set measurement parameters in the [Parameters] tab. The [Parameters] tab has 2 subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 83 If values are out of displayable range, "######" is displayed. "6.2.3 Quantitation by K-factor Method" P.57 [Data] Displays photometric value of the current sample. Displays the value obtained by multiplying photometric value [K * Data] by K Factor. UV-1900i Operation Guide...
  • Page 84 999 at the maximum. When 999 is exceeded, it returns to 1. • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "6.2 Measurement" P.55. UV-1900i Operation Guide...

  • Page 85: Measurement

    This step can be skipped when the same parameters are used for the measurement. The photometric value will be set to 0 Abs (100 % T). Tap [Start]. The [Photo. Table] tab is displayed and measurement starts using set measurement parameters. Operation other than [Stop] is disabled during measurement. UV-1900i Operation Guide...

  • Page 86: Checking Measurement Results

    Factor Settings] subtab does not change. Tap this button to enable the function (highlighted in blue) to Multiple select multiple measurement results. Selection button Tap a selected measurement result to cancel the selection when multiple results are selected. UV-1900i Operation Guide...

  • Page 87: Quantitation By K-Factor Method

    Tap the input field of [K Factor] in the [WL / K Factor Settings] subtab ("[WL / K Factor Settings] subtab" P.53). Numeric keypad is displayed ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Enter K Factor. The value in the input filed of [K Factor] is changed. UV-1900i Operation Guide...
  • Page 88 Blank correction starts. After completion, proceed to the next step. Place the unknown sample in the sample compartment. Tap [Start]. Measurement starts. After measurement, photometric value and the result of quantitation (K ´ photometric value) are displayed. UV-1900i Operation Guide...
  • Page 89 Select measurement results to be recalculated. Tap [Recalc.]. Numeric keypad is displayed. Enter K Factor. "4.2.2 Numeric Input Screen (10-button Keypad)" P.29 Measurement result is recalculated. And the value in [K] changes to reset value. UV-1900i Operation Guide...

  • Page 90: Printing Measurement Results

    The date and time when the first measurement is completed Date and time are printed. Measurement Measurement parameters are printed. parameters In order from left, sample number, set sample name, Results photometric value, and concentration are printed. UV-1900i Operation Guide...

  • Page 91: Manual Print

    All measurement results, or all the data within the currently-loaded table data file will be printed as a numeric data table. Tap [Print]. Tap [Measured data]. A numeric data table is printed. The format is the same as "6.3.1 Auto Print" P.60. UV-1900i Operation Guide...

  • Page 92: Photometric 8L 7.1 Setting Measurement Parameters

    • The measurement data at each wavelength is rounded to four decimal places (i.e. the fifth decimal place is rounded off). • "A" in the equations depends on [Photometric Type]. For [Absorbance (Abs)], [Transmittance (%T)], and [Reflectance (%R)], "A", "T", and "R" shall be used, respectively. UV-1900i Operation Guide...
  • Page 93 The measurement screen is displayed. Set measurement parameters in the [Parameters] tab. The [Parameters] tab has 3 subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 94 Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Wavelength The measurement will be carried out in the order of [WL1], [WL2],…[WLn]. When all measurements are completed, the UV-1900i automatically goes to [WL1]. UV-1900i Operation Guide...
  • Page 95 In the methods that calculate the concentration by Factor Settings multiplying measurement data by the factor, such as K Factor method, values are displayed in the range of -9999.9 to 9999.9. If values are out of displayable range, "######" is displayed. UV-1900i Operation Guide...
  • Page 96 999 at the maximum. When 999 is exceeded, it returns to 1. • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "7.2 Measurement" P.68. UV-1900i Operation Guide...
  • Page 97 Transmittance at measurement wavelength WLn (n = 1 to 4) Rn (n = 1 to 4): Reflectance at measurement wavelength WLn (n = 1 to 4) Kn (n = 1 to 5): Factor that is specifiable between -9999.9 and 9999.9. UV-1900i Operation Guide...

  • Page 98: Measurement

    This step can be skipped when the same parameters are used for the measurement. Tap [Start]. The [Photo. Table] tab is displayed and measurement starts using set measurement parameters. Operation other than [Stop] is disabled during measurement. UV-1900i Operation Guide...

  • Page 99: Checking Measurement Results

    • Tap [Save Table] to save measured data ("4.3 Saving Files" P.32). • Tap [Load Table] to load saved table data file ("4.4 Loading Files" P.37). • Tap [Print] to print measured data ("7.3.2 Manual Print" P.71). UV-1900i Operation Guide...

  • Page 100: Printing Measurement Results

    The above figure is an example when [No. of WLs] is set at [2]. When more than [3] is selected, they will be printed in the following format. Results UV-1900i Operation Guide...

  • Page 101: Manual Print

    All measurement results, or all the data within the currently-loaded table data file will be printed as a numeric data table. Tap [Print]. Tap [Measured data]. A numeric data table is printed. The format is the same as "7.3.1 Auto Print" P.70. UV-1900i Operation Guide...

  • Page 102: Spectrum

    The measurement screen is displayed. Set measurement parameters in the [Parameters] tab. The [Parameters] tab has 2 subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 103 Auto File function, only the latest-acquired data can be printed. Therefore, if you wish to obtain the printouts of these measurement results, enable [Auto Print] function. Subtab Switches setting items in the [Parameters] tab. UV-1900i Operation Guide...
  • Page 104 (and still does not exceed 2001). The [Data Interval] equation is shown below: the maximum value out of 0.05/0.1/0.2/0.5/1.0/2.0 that meets [2001 > Scan Range/Data Interval] When [Scan Speed] (7) is set to [Survey], [1.0 nm] and [2.0 nm] are available. UV-1900i Operation Guide...
  • Page 105 This is used to display multiple curve data for measurement results of repeated measurement. [Display Mode] When [Sequential] is selected, only one curve data is displayed. Even if [Overlay] is selected, only the latest acquired data can be saved or processed. UV-1900i Operation Guide...
  • Page 106 P.29). • If the time required for scanning is shorter than [Meas. Interval] the set value, the UV-1900i will wait for the next scan start. During this waiting period, the next scanning count and the remaining waiting time are displayed.

  • Page 107: Measurement

    This step can be skipped when the same parameters are used for the measurement. Tap [Start]. The [Measurement] tab is displayed and measurement starts using set measurement parameters. During measurement, a spectral waveform is plotted in real time. Operation other than [Stop] is disabled during measurement. UV-1900i Operation Guide...

  • Page 108: Checking Measurement Results

    8 Spectrum 8.2.2 Checking Measurement Results After the measurement has finished (stopped), the graph can be scaled up/down. Name Description Graph Displays the spectral waveform. UV-1900i Operation Guide...
  • Page 109 • The cursor can be moved to the left/right with button. Hold down the button to move the cursor continuously. • The wavelength and photometric value at intersection of the spectral waveform and the cursor are displayed. UV-1900i Operation Guide...
  • Page 110 When multiple spectral waveforms are overlaid on the graph, the operation is applied to the latest acquired or loaded waveform. • Tap [Save Curve] to save measured data ("4.3 Saving Files" P.32). • Tap [Print] to print measured data ("8.3.2 Manual Print ([Measurement] Tab)" P.85). UV-1900i Operation Guide...
  • Page 111 Processing data of measurement results Tap [Operations] to apply the following operations to obtained spectral waveform. For details, see "14 Data Processing" P.195. • Arithmetic • Derivative • Peak Pick Operations • Point Pick • Data Print • Peak Area UV-1900i Operation Guide...

  • Page 112: Auto File Function

    Tap the input field of the File Name and enter the name using the text input screen ("4.2.1 Text Input Screen (Keyboard)" P.27). The file name specified here is assigned to the saved file with sequential numbers from "01" attached. UV-1900i Operation Guide...
  • Page 113 8.2 Measurement Tap [Start]. Measurement starts. Upon completion of the measurement, the file with specified name is saved to the selected destination. UV-1900i Operation Guide...

  • Page 114: Printing Measurement Results

    It includes different axes but the content is equal. Graph When [Display Mode] ("[Measurement Settings] subtab" P.75) is set to [Overlay] and a hard copy printer is used, multiple curves displayed on the screen are printed as shown on the screen. UV-1900i Operation Guide...

  • Page 115: Manual Print ([Measurement] Tab)

    8.3.2 Manual Print ([Measurement] Tab) The graph, date, time, and measurement parameters can be printed for the curve data files on the [Measurement] tab. Tap [Print]. Tap [Measured data]. The graph, date, time, and measurement parameters are printed. UV-1900i Operation Guide...
  • Page 116 The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters • Data at multiple sampling points can be listed and printed ("14.6 Point Pick" P.219). • All data values can be printed on the Operations menu ("14.8 Data Print" P.233). UV-1900i Operation Guide...

  • Page 117: Manual Print ([View] Tab)

    8.3.3 Manual Print ([View] Tab) The graph, date, time, and measurement parameters can be printed for the curve data files loaded on the [Display] tab. Tap [Print]. Tap [Loaded Data]. The graph, date, time, and measurement parameters are printed. UV-1900i Operation Guide...
  • Page 118 The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters • Data at multiple sampling points can be listed and printed ("14.6 Point Pick" P.219). • All data values can be printed on the Operations menu ("14.8 Data Print" P.233). UV-1900i Operation Guide...

  • Page 119: Quantitation

    The measurement results may be displayed as a list. A single list can include a maximum of 400 measurement results. This data may be saved as a single curve data file. UV-1900i Operation Guide...

  • Page 120: Operation Flow

    Sample is Known" P.101 Sample is Unknown" P.105 È [Formula] is set to... [2 Wavelengths], [3 Wavelengths], or [1 Wavelength] [Derivative] È È "9.6.2 Performing Measurement (2 "9.6.1 Performing Measurement Wavelengths, 3 Wavelengths, or (One-wavelength Method)" P.113 Derivative)" P.114 UV-1900i Operation Guide...

  • Page 121: Setting Measurement Parameters

    The [Parameters] tab has four subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). For procedures to save or load parameters, see "9.5 Saving and Loading Parameters Files in the Quantitation Mode" P.112. UV-1900i Operation Guide...
  • Page 122 Specifies the formula to be used. [Formula] Tap to select [1 Wavelength], [2 Wavelengths], [3 Wavelengths], or [Derivative]. Confirms the resettings. This is enabled when you return to the [Resettings] tab in which the setting is completed. UV-1900i Operation Guide...
  • Page 123 [Wavelength] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Specifies the order of derivative. [Order] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 124 If values are out of displayable range, "######" is displayed. Specifies the constant B. Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). [Conc.] Displays the equation to calculate sample concentration. UV-1900i Operation Guide...
  • Page 125 When this method is selected, the other items in the [Calib. Curve Settings] subtab do not need to be set. UV-1900i Operation Guide...
  • Page 126 1 to [Order of Curve] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Each tap of this key toggles between ON and OFF of Zero [Zero Interception. Intercepton] OFF: UV-1900i Operation Guide...
  • Page 127 Unit [ ] ] and then enter the desired unit in [Unit (User-defined)]. Specifies the desired concentration unit for the measurement [Unit result. (User-defined)] Tap the input field to display text input screen ("4.2.1 Text Input Screen (Keyboard)" P.27). UV-1900i Operation Guide...
  • Page 128 The value of [Start Number] (up to 999) is added to this, therefore, the number of characters of sample name is 18 at the maximum. • The value entered in [Start Number] increases to 999 at the maximum. When 999 is exceeded, it returns to 1. UV-1900i Operation Guide...
  • Page 129 After setting measurement parameters, set attachments according to "9.3 Setting Attachments" P.100. Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). For procedures to save or load parameters, see "9.5 Saving and Loading Parameters Files in the Quantitation Mode" P.112. UV-1900i Operation Guide...

  • Page 130: Setting Attachments

    [1 Wavelength] is selected in [Formula] P.113 When [K Factor] is selected in [Method] "9.6.2 Performing Measurement and [2 Wavelengths], [3 Wavelengths], or [Start] (2 Wavelengths, 3 Wavelengths, [Derivative] is selected in [Formula] or Derivative)" P.114 UV-1900i Operation Guide...

  • Page 131: Creating A Calibration Curve

    When [No. of Samples] in the [Calib. Curve Settings] subtab is set to 2 or more, [Go to (Sample Number)] and [Back to (Sample Number)] are displayed. Tap [OK] after entering the concentrations of all the standard samples. UV-1900i Operation Guide...
  • Page 132 As with the concentrations, tap [OK] after entering the absorbance of all the standard samples. Tap [Cancel] to cancel the entry and return to the [Parameters] tab. You can check entered concentrations and absorbance on the screen. UV-1900i Operation Guide...
  • Page 133 [Formula]. See below. • [1 Wavelength] is selected: "9.6.1 Performing Measurement (One-wavelength Method)" P.113 • Either of [2 Wavelengths], [3 Wavelengths], or [Derivative] is selected: "9.6.2 Performing Measurement (2 Wavelengths, 3 Wavelengths, or Derivative)" P.114 UV-1900i Operation Guide...
  • Page 134 For the quadratic or cubic equation, the value of K3Ci K2Ci + K1Ci + K0 is used for the value Ai. Confirms changes and closes the window. Displayed calibration [Apply] curve will be changed. [Cancel] Discards changes and closes the window. UV-1900i Operation Guide...

  • Page 135: When Absorbance Of The Standard Sample Is Unknown

    When [No. of Samples] in the [Calib. Curve Settings] subtab is set to 2 or more, [Go to (Sample Number)] and [Back to (Sample Number)] are displayed. Tap [OK] after entering the concentrations of all the standard samples. UV-1900i Operation Guide...
  • Page 136 9 Quantitation Tap [Measure Cell 1]. Tap [Auto Zero] or [Base Corr.] setting a blank sample in the cell. UV-1900i Operation Guide...
  • Page 137 When [Repetitions] in the [Parameters] tab is set to 2 or more, tap [Next Sample]. Repeat step 1 to 3 until all the standard samples are measured. Tap [Calib. Curve]. The tab switches to the [Calib. Curve] tab and you can check the calibration curve. UV-1900i Operation Guide...
  • Page 138 A linear, quadratic, or cubic equation can be used as the [Order] calibration curve equation, therefore, setting range is 1 to 3. Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 139 The following procedures vary according to the settings of [Formula]. See below. • [1 Wavelength] is selected: "9.6.1 Performing Measurement (One-wavelength Method)" P.113 • Either of [2 Wavelengths], [3 Wavelengths], or [Derivative] is selected: "9.6.2 Performing Measurement (2 Wavelengths, 3 Wavelengths, or Derivative)" P.114 UV-1900i Operation Guide...
  • Page 140 Measuring Multi-cell Continuously When using a multi-cell holder (optional), multiple standard samples can be measured continuously. Perform step 1 and 2 in "9.4.2 When Absorbance of the Standard Sample is Unknown" P.105. Tap [Measure Multi-cell Cont.]. UV-1900i Operation Guide...
  • Page 141 ("19.2 Settings of [Multi-Cell (6 Cells)], [MMC (8 Cells)], [MMC (16 Cells)], and [CPS]" P.451) and then stops. When tapping [Start] after that, the UV-1900i starts sequential measurements and stops again. Repeat it until the same number of measurements as [No. of Samples] ("[Calib. Curve Settings] subtab"...

  • Page 142: Saving And Loading Parameters Files In The Quantitation Mode

    • The settings in the [Parameters] and [Attachments] tabs will be the settings in the loaded file. • The settings in the [Std. Table] and [Calib. Curve] tabs are cleared. • The [Attachments] tab is displayed on the screen. UV-1900i Operation Guide...

  • Page 143: Measurement

    Measurement starts using set measurement parameters. Operation other than [Stop] is disabled during measurement. When [Stop] is tapped, the average value of repeated measurements is not calculated. After the measurement, see the results according to "9.6.3 Checking Measurement Results" P.115. UV-1900i Operation Guide...

  • Page 144: Performing Measurement (2 Wavelengths, 3 Wavelengths, Or Derivative)

    Measurement starts using set measurement parameters. Operation other than [Stop] is disabled during measurement. When [Stop] is tapped, the average value of repeated measurements is not calculated. After the measurement, see the results according to "9.6.3 Checking Measurement Results" P.115. UV-1900i Operation Guide...

  • Page 145: Checking Measurement Results

    The four buttons on the right operate as follows: The number of results and • selection/page Moves to the previous or next page when the list of button measurement results continues for more than one page. • Selects the previous or next measurement results. UV-1900i Operation Guide...
  • Page 146 • Tap [Save Table] to save measured data ("4.3 Saving Files" P.32). • Tap [Load Table] to load saved table data file ("4.4 Loading Files" P.37). • Tap [Print] to print measured data ("9.7.2 Manual Print" P.118). UV-1900i Operation Guide...

  • Page 147: Printing Measurement Results

    The above figure shows an example when [Formula] is set to [1 Wavelength] or [Derivative]. When [2 Wavelengths] or [3 Wavelengths] is selected, the following format is used according to the number of wavelengths (The figure shows an example of [2 Wavelengths]). Results UV-1900i Operation Guide...

  • Page 148: Manual Print

    • When selecting [Unknown Sample Table], only the unknown sample table is printed. The printing format is the same as shown in "9.7.1 Auto Print" P.117. • When selecting [All Tables], the standard sample table, calibration curve, and unknown sample table are printed. UV-1900i Operation Guide...

  • Page 149: Quantitation Method

    Thus, the information on the target component remains on the equation. Normally, the absorbance wavelength for the target component is set for WL The following figure shows an example of two-wavelength measurement. Quantitation measurement is then performed using these parameters. UV-1900i Operation Guide...
  • Page 150 S, T and U of the interfering component are connected by a single straight line, then leaving only the information for the target component. Normally, the absorbance wavelength for the target component is set for WL Quantitation measurement is then performed using these parameters. UV-1900i Operation Guide...

  • Page 151: Derivative Quantitation Method

    Derivative in the quantitation mode is calculated from 17 points of data before and after the center of the set wavelength. The derivative wavelength difference DWL is constant at 0.8 nm. In addition, the order of derivative can be set from 1st through 4th. UV-1900i Operation Guide...

  • Page 152: Kinetics 10.1 Setting Measurement Parameters

    Activity values are obtained by multiplying the range of change by four factor values. The equation is shown below: (Activity value) = (Factor 1) ´ (Factor 2) ´ (Factor 3) ´ (Factor 4) ´ (Rate of change) UV-1900i Operation Guide...

  • Page 153: Setting Measurement Parameters

    The measurement screen is displayed. Set measurement parameters in the [Parameters] tab. The [Parameters] tab has four subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 154 Specifies the range of Y-axis of the graph ("[Reaction Curve] tab" P.129). Enter the lower and upper limit in the left and [Record Range] right fields, respectively. Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 155 • Up to 2001 data points can be obtained. When the measurement points exceed 2001, the setting cannot be accepted. UV-1900i Operation Guide...
  • Page 156 The input range is as follows: • Start time: 0 to ((Total Time) - (Data Interval)) • End time: (Data Interval) to ((Total Time) - (Start time)) Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 157 999 at the maximum. When 999 is exceeded, it returns to 1. • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "10.2 Measurement" P.128. UV-1900i Operation Guide...

  • Page 158: Measurement

    • When [Base Corr.] is tapped, baseline correction will be carried out. Tap [Start]. The [Reaction Curve] tab is displayed and measurement starts using set measurement parameters. During measurement, a reaction curve is plotted in real time. And, operations other than [Stop] is disabled. UV-1900i Operation Guide...

  • Page 159: Checking Measurement Results

    [Reaction Curve] and [Activity] tabs. [Reaction Curve] tab Name Description Displays the reaction curve. Graph Blue and green vertical lines indicate the start and end time of the activity calculation range, respectively. UV-1900i Operation Guide...
  • Page 160 • For a multiple cell measurement with multi-cell holder, the Cell Number Selection screen appears for selecting the corresponding to the reaction curve. Tap to select the target reaction curve to display the wavelength and absorbance. UV-1900i Operation Guide...
  • Page 161 Display Reset Cancels the latest change of display range or scale-up/down. button (once) Cancels all changes of display range or scale-up/down and Display Reset returns the graph to the state immediately after the button (all) measurement. UV-1900i Operation Guide...
  • Page 162 Tap a selected measurement result to cancel the selection when multiple results are selected. Opens the screen to reset the activity calculation range changing the start and end time. [Recalc.] For details, see "Resetting the activity calculation range" P.133. Delete button Deletes selected measurement results. UV-1900i Operation Guide...
  • Page 163 Blue and green vertical lines on the graph indicate the start and end time of the activity calculation range, respectively. Buttons relating Function of each button is the same as the [Reaction Curve] tab to graph display ("[Reaction Curve] tab" P.129). UV-1900i Operation Guide...
  • Page 164 Recalculation will be carried out based on changed start and end time. [Execute] Tapping this button closes this screen and the result of recalculation is displayed on the [Activity] tab. Closes this screen and returns to the [Activity] tab. Changes will [Close] be discarded. UV-1900i Operation Guide...

  • Page 165: Printing Measurement Results

    Print Prints time and absorbance at all plots (point where data is obtained) of the graph displayed in the [Reaction Curve] Curve total plot tab as a numerical data table. "10.3.4 Manual Print (Curve Total Plot)" P.141 UV-1900i Operation Guide...

  • Page 166: Auto Print

    The date and time when the first measurement is completed Date and time are printed. Measurement Measurement parameters are printed. parameters Measurement In order from left, sample number, set sample name, results photometric value, concentration, and activity value are printed. UV-1900i Operation Guide...

  • Page 167: Manual Print (Table Data)

    10.3 Printing Measurement Results 10.3.2 Manual Print (Table Data) All measurement results, or all the data within the currently-loaded table data file (activity value) will be printed as a numeric data table. Tap [Print]. Tap [Table data]. UV-1900i Operation Guide...
  • Page 168 Measurement Measurement parameters are printed. parameters In order from top, sample name, activity calculation range, photometric value, reaction speed, and activity are printed. For loaded data, "Reload Data" is indicated in the header. Measurement results UV-1900i Operation Guide...

  • Page 169: Manual Print (Curve Data)

    The graph, date, time, and measurement parameters of [Reaction Curve] tab can be printed for all measurement results and all loaded curve data files. Grid type of the graph is selectable when using a commercially available printer ("15.13 Setting the Printer" P.253). Tap [Print]. Tap [Curve data]. UV-1900i Operation Guide...
  • Page 170 However, when using a commercially available printer, only the curve obtained in the latest measurement can be printed. Date and time The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters UV-1900i Operation Guide...

  • Page 171: Manual Print (Curve Total Plot)

    Prints time and absorbance at all plots (point where data is obtained) of the graph displayed in the [Reaction Curve] tab as a numerical data table for all measurement results and all loaded curve data files. Tap [Print]. Tap [Curve plot]. UV-1900i Operation Guide...
  • Page 172 The above figure shows an example when the measurement is carried out with the standard sample compartment. When using optional multi-cell holder, the format changes according to the number of cells as follows: Measurement results UV-1900i Operation Guide...

  • Page 173: Kinetics Rate

    (%) set in the criteria. |{(change in absorbance at the last rate interval)-(change in absorbance at the rate interval)} / (change in absorbance at the last rate interval)| ´ 100 < (criteria) UV-1900i Operation Guide...

  • Page 174: Setting Measurement Parameters

    The measurement screen is displayed. Set measurement parameters in the [Parameters] tab. The [Parameters] tab has three subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 175 Specifies the range of Y-axis of the graph ("[Reaction Curve] tab" P.149). Enter the lower and upper limit in the left and [Record Range] right fields, respectively. Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 176 Specifies the interval to acquire measurement data. This is [Data Interval enabled when [Integer Input] is selected in [Data Interval]. (Integer Input)] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 177 ´ 100 < (criteria) • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "11.2 Measurement" P.148. UV-1900i Operation Guide...

  • Page 178: Measurement

    The measurement results will be displayed in real time. Operation other than [Stop] is disabled during measurement. To carry out the measurement while checking the reaction curve, switch the screen to the [Reaction Curve] tab and then tap [Start]. UV-1900i Operation Guide...

  • Page 179: Checking Measurement Results

    11.2 Measurement 11.2.2 Checking Measurement Results When the measurement is completed, you can edit measurement results on the [Reaction Curve] and [Rate] tabs. [Reaction Curve] tab Name Description Graph Displays the reaction curve. UV-1900i Operation Guide...
  • Page 180 • The cursor can be moved to the left/right with button. Hold down the button to move the cursor continuously. • The wavelength and photometric value at intersection of the reaction curve and the cursor are displayed. UV-1900i Operation Guide...
  • Page 181 Display Reset Cancels the latest change of display range or scale-up/down. button (once) Cancels all changes of display range or scale-up/down and Display Reset return the reaction curve to the state immediately after the button (all) measurement. UV-1900i Operation Guide...
  • Page 182 In the Kinetics Rate mode, the rate is not calculated after loading saved curve, therefore, curve data files cannot be loaded. • Tap [Print] to print measured data ("11.3.3 Manual Print (Curve data)" P.155, "11.3.2 Manual Print (Table Data)" P.154, "11.3.4 Manual Print (Curve Total Plot)" P.157). UV-1900i Operation Guide...

  • Page 183: Printing Measurement Results

    If a hard copy printer (optional) is connected, the measurement results will be printed on the printer for every measurement. After the first measurement, date, time, measurement parameters, and the first measurement results will be printed. After that, the measurement results will be printed for every measurement. UV-1900i Operation Guide...

  • Page 184: Manual Print (Table Data)

    All measurement results, or all the data within the currently-loaded table data file (rate) will be printed as a numeric data table. Tap [Print]. Tap [Table data]. A numeric data table is printed. The format is the same as "11.3.1 Auto Print" P.153. UV-1900i Operation Guide...

  • Page 185: Manual Print (Curve Data)

    The graph, date, time, and measurement parameters of [Reaction Curve] tab can be printed for all measurement results and all loaded curve data files. Grid type of the graph is selectable when using a commercially available printer ("15.13 Setting the Printer" P.253). Tap [Print]. Tap [Curve data]. UV-1900i Operation Guide...
  • Page 186 However, when using a commercially available printer, only the curve obtained in the latest measurement can be printed. Date and time The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters UV-1900i Operation Guide...

  • Page 187: Manual Print (Curve Total Plot)

    Prints time and absorbance at all plots (point where data is obtained) of the graph displayed in the [Reaction Curve] tab as a numerical data table for all measurement results and all loaded curve data files. Tap [Print]. Tap [Curve plot]. UV-1900i Operation Guide...
  • Page 188 Description Date and time The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters Measurement In order from left, elapsed time and absorbance at each plot results are printed. UV-1900i Operation Guide...

  • Page 189: Time Course

    The measurement screen is displayed. Set measurement parameters in the [Parameters] tab. The [Parameters] tab has two subtabs. Select the subtab and make necessary settings changes. Tap [Load Params] to load saved measurement parameters ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 190 Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Specifies the range of Y-axis of the graph displayed during the measurement. [Record Range] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 191 [OFF] is used to introduce the external light source into the spectrometer when measuring the energy change of light sources other than those equipped in the standard UV-1900i. [Light Source] When it is selected, both the WI and D2 lamps are turned OFF and the light source mirror turns to the third light source.
  • Page 192 Input Screen (10-button Keypad)" P.29). • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "12.2 Measurement" P.163. UV-1900i Operation Guide...
  • Page 193 Performs measurement using set measurement parameters. Tap [Start]. The [Measurement] tab is displayed and measurement starts using set measurement parameters. During measurement, a time curve is plotted in real time. Operation other than [Stop] is disabled during measurement. UV-1900i Operation Guide...
  • Page 194 12 Time Course 12.2.2 Checking Measurement Results After the measurement has finished (stopped), the graph can be scaled up/down. Name Description Graph Displays the time curve. UV-1900i Operation Guide...
  • Page 195 • For a multiple cell measurement with multi-cell holder, the Cell Number Selection screen appears for selecting the corresponding to the time curve. to select the target time curve to display the wavelength and absorbance. UV-1900i Operation Guide...
  • Page 196 When multiple time curves are overlaid on the graph, the operation is applied to the latest acquired or loaded time curve. • Tap [Save Curve] to save measured data ("4.3 Saving Files" P.32). • Tap [Print] to print measured data ("12.3.2 Manual Print ([Measurement] Tab)" P.171). UV-1900i Operation Guide...
  • Page 197 • Multiple curve data files can be loaded and overlaid, if they have the same measurement parameters. • When a curve data file of the Time Course mode is loaded on [Data File] of the Mode Menu screen, this tab appears on the screen. UV-1900i Operation Guide...
  • Page 198 Data processing cannot be applied to multiple reaction curves. For a multiple cell measurement with multi-cell holder, the Cell Number Selection screen appears for selecting the corresponding curve. to select the target reaction curve for the data processing. UV-1900i Operation Guide...
  • Page 199 When the [Auto Print] function is enabled in the [Wavelength Settings] subtab ("[Wavelength Settings] subtab" P.160), the date, time, and graph are automatically printed after the measurement. Name Description Date and time The date and time of the end of the measurement are printed. UV-1900i Operation Guide...
  • Page 200 When using a hard copy printer, multiple curves displayed on the screen are printed as shown on the screen. However, when using a commercially available printer, only the curve obtained in the latest measurement can be printed. UV-1900i Operation Guide...
  • Page 201 The graph, date, time, and measurement parameters can be printed for the curve data files on the [Measurement] tab. When multiple time curves are overlaid on the graph, the latest acquired or loaded time curve is printed. Tap [Print]. Tap [Measured data]. UV-1900i Operation Guide...
  • Page 202 The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters • Data at multiple sampling points can be listed and printed ("14.6 Point Pick" P.219). • All data values can be printed on the Operations menu ("14.8 Data Print" P.233). UV-1900i Operation Guide...
  • Page 203 The graph, date, time, and measurement parameters can be printed for the curve data files loaded on the [Display] tab. When multiple time curves are overlaid on the graph, the latest acquired or loaded time curve is printed. Tap [Print]. Tap [Loaded Data]. UV-1900i Operation Guide...
  • Page 204 The date and time of the end of the measurement are printed. Measurement Measurement parameters are printed. parameters • Data at multiple sampling points can be listed and printed ("14.6 Point Pick" P.219). • All data values can be printed on the Operations menu ("14.8 Data Print" P.233). UV-1900i Operation Guide...
  • Page 205 This method determines protein concentration directly from absorbance value and absorptivity in the ultraviolet region, without using any color reagent. This method normally measures absorbance at a wavelength UV Method of 280 nm. "13.1.3 Setting Measurement Parameters (UV Method)" P.184 UV-1900i Operation Guide...
  • Page 206 • Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. & Klenk, D. C. (1985) Anal. Biochem. 150, 76-85. UV-1900i Operation Guide...
  • Page 207 Parameters to be specified depend on the quantitation method. See below. • "13.1.1 Setting Measurement Parameters (DNA Quantitation)" P.178 • "13.1.2 Setting Measurement Parameters (Lowry Method, BCA Method, CBB Method, Biuret Method)" P.183 • "13.1.3 Setting Measurement Parameters (UV Method)" P.184 UV-1900i Operation Guide...
  • Page 208 WL1 and WL2 are defined. Each tap of this key toggles between ON and OFF of the background correction function. [Background OFF: Correction] When it is turned ON, [BG WL] is subtracted from [WL1] and [WL2]. UV-1900i Operation Guide...
  • Page 209 When [Type A] or [Type B] is selected in [Wavelength Pattern], [WL2] wavelengths for the measurement are displayed. When [Manual [BG WL] Input] is selected, tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29) and enter the desired wavelength. UV-1900i Operation Guide...
  • Page 210 When [Type A] or [Type B] is selected in [Factor Patterns], the [K1] to [K4] values are displayed. When [Manual Input] is selected, tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29) and enter the desired value. UV-1900i Operation Guide...
  • Page 211 • A2: Absorbance at [WL2] (the value obtained by subtracting the absorbance of [BG WL] when the background correction function is enabled) Name Description [Absorbance Displays the equation to calculate absorbance ratio. Ratio] [DNA Displays the equation to calculate DNA concentration. Concentration] [Protein Displays the equation to calculate protein concentration. Concentration] UV-1900i Operation Guide...
  • Page 212 1. • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "13.2.1 Performing Measurement (DNA Quantitation)" P.187. UV-1900i Operation Guide...
  • Page 213 Other operations are the same as the Quantitation mode. For setting measurement parameters, performing measurement, and checking and printing measurement results, see below. • "9.1 Operation Flow" P.90 • "9.2 Setting Measurement Parameters" P.91 • "9.6 Measurement" P.113 • "9.7 Printing Measurement Results" P.117 UV-1900i Operation Guide...
  • Page 214 [Concentration] Displays the equation to calculate protein concentration. Returns the settings to the defined values. The defined values are shown below. • [Absorption Coefficient]: 0.667 [Define] • [Wavelength]: 280.0 nm • [Repetitions]: 1 • [Unit]: mg/ml UV-1900i Operation Guide...
  • Page 215 Unit [ ] ] and then enter the desired unit in [Unit (User-defined)]. Specifies the desired concentration unit for the measurement [Unit result. (User-defined)] Tap the input field to display text input screen ("4.2.1 Text Input Screen (Keyboard)" P.27). UV-1900i Operation Guide...
  • Page 216 1. • To set attachments, see "19 Setting Attachments" P.449. • Tap [Save Params] to save measurement parameters ("4.3 Saving Files" P.32). After setting measurement parameters, perform measurement according to "13.2.3 Performing Measurement (UV Method)" P.189. UV-1900i Operation Guide...
  • Page 217 Tap [Start]. The [Unk. Table] tab is displayed and measurement starts using set measurement parameters. Operation other than [Stop] is disabled during measurement. After the measurement, see the results according to "13.2.2 Checking Measurement Results (DNA Quantitation)" P.188. UV-1900i Operation Guide...
  • Page 218 • Tap [Save Table] to save measured data ("4.3 Saving Files" P.32). • Tap [Load Table] to load saved table data file ("4.4 Loading Files" P.37). • Tap [Print] to print measured data ("13.3.2 Manual Print" P.193). UV-1900i Operation Guide...
  • Page 219 Tap [Start]. The [Unk. Table] tab is displayed and measurement starts using set measurement parameters. Operation other than [Stop] is disabled during measurement. After the measurement, see the results according to "13.2.4 Checking Measurement Results (UV Method)" P.190. UV-1900i Operation Guide...
  • Page 220 The four buttons on the right operate as follows: The number of results and • selection/page Moves to the previous or next page when the list of button measurement results continues for more than one page. • Selects the previous or next measurement results. UV-1900i Operation Guide...
  • Page 221 • Tap [Save Table] to save measured data ("4.3 Saving Files" P.32). • Tap [Load Table] to load saved table data file ("4.4 Loading Files" P.37). • Tap [Print] to print measured data ("13.3.2 Manual Print" P.193). UV-1900i Operation Guide...
  • Page 222 Date and time are printed. Measurement Measurement parameters are printed. parameters Measurement The measurement results are printed. results The printing format in the other quantitation methods is the same as the Quantitation mode ("9.7.1 Auto Print" P.117). UV-1900i Operation Guide...
  • Page 223 All measurement results, or all the data within the currently-loaded table data file will be printed as a numeric data table. n For DNA Quantitation/UV Method Tap [Print]. Tap [Measured data]. A numeric data table is printed. The format is the same as "13.3.1 Auto Print" P.192. UV-1900i Operation Guide...
  • Page 224 13 Bio Method n For Other Quantitation Methods The printing procedures are the same as the Quantitation mode. "9.7.2 Manual Print" P.118 UV-1900i Operation Guide...

  • Page 225: Data Processing

    Display the curve data and tap [Operations]. To display the curve data: • Carry out measurement in the Spectrum or Time Course mode. • Load a curve data file in the Spectrum or Time Course mode ("4.4 Loading Files" P.37). UV-1900i Operation Guide...
  • Page 226 [Peak Area] "14.7 Peak Area" P.227 Prints measurement parameters and data at each sampling point of curve data. [Data Print] "14.8 Data Print" P.233 [Redo] Restores the original data before data processing. [Cancel] Returns to the Operations screen. UV-1900i Operation Guide...
  • Page 227 Hold down the button to move the cursor continuously. Operation • The horizontal values (wavelength or time) and photometric Switching button value at intersection of the curve and the cursor are displayed. This button is not displayed on the Peak Area and Point Pick screens. UV-1900i Operation Guide...
  • Page 228 Display Reset Cancels the latest change of display range or scale-up/down. button (once) Cancels all changes of display range or scale-up/down and Display Reset returns the graph to the state immediately after the button (all) measurement. UV-1900i Operation Guide...

  • Page 229: Arithmetic Operations

    14.3 Arithmetic Operations 14.3 Arithmetic Operations Performs arithmetic operations between curve data and values or between curve data. 14.3.1 Performing Arithmetic Operations Tap [Arithmetic Operations]. UV-1900i Operation Guide...
  • Page 230 • For data with different horizontal axis range or sampling intervals, calculation is performed for consistent range ("Operation for curve data with different horizontal axis range or sampling intervals" P.202). [Execute] Performs operation using set parameters. [Cancel] Returns to the Operations screen. UV-1900i Operation Guide...
  • Page 231 Tap this button to perform recalculation after changing the parameters. Returns to the Operations screen. [Close] Tap this button to exit the screen. Buttons relating For details, see "14.2 Operation of Common Buttons" P.197. to graph display UV-1900i Operation Guide...
  • Page 232 For example, wavelength range is 400 nm to 600 nm for the original data and 420 nm to 620 nm for the operation target data. In this case, wavelength range of the operation result data is 420 nm to 600 nm. UV-1900i Operation Guide...
  • Page 233 0.2 nm. 14.3.2 Printing the Results (Arithmetic Operations) The results of arithmetic operations can be printed out. Grid type of the graph is selectable when using a commercially available printer ("15.13 Setting the Printer" P.253). Tap [Print]. UV-1900i Operation Guide...
  • Page 234 The results are printed on the printer. Name Description Graph The graph after calculation is printed. The date and time when measurement of the original data is Date and time finished are printed. Measurement Measurement parameters of the original data are printed. parameters UV-1900i Operation Guide...
  • Page 235 The greater the coefficient, the greater the derivative wavelength (time) difference, and the less the noise. However, specifying a coefficient larger than appropriate deteriorates the resolution of the derivative spectrum. The two elements of noise and resolution should be considered when determining the coefficient value. UV-1900i Operation Guide...
  • Page 236 14 Data Processing 14.4.2 Performing Derivative Processing Tap [Derivative]. UV-1900i Operation Guide...
  • Page 237 Tap it to display numeric keypad ("4.2.2 Numeric Input Screen [DT (N)] (10-button Keypad)" P.29). [Execute] Performs operation using set parameters. [Cancel] Returns to the Operations screen. Tap [Execute]. The operation is performed and the graph is updated. UV-1900i Operation Guide...
  • Page 238 Tap this button to perform recalculation after changing the parameters. Returns to the Operations screen. [Close] Tap this button to exit the screen. Buttons relating For details, see "14.2 Operation of Common Buttons" P.197. to graph display UV-1900i Operation Guide...
  • Page 239 14.4 Derivative n Example of smoothing processing Dl(N): 1 Raw data Dl(N): 5 Dl(N): 9 UV-1900i Operation Guide...
  • Page 240 400 nm to 500 nm, it is assumed for the calculation that all data sets at wavelengths shorter than 400 nm are 0.1 Abs, and those at wavelengths longer than 500 nm are 0.2 Abs. UV-1900i Operation Guide...
  • Page 241 14.4 Derivative 14.4.3 Printing the Results (Derivative) The results of derivative operations can be printed out. Grid type of the graph is selectable when using a commercially available printer ("15.13 Setting the Printer" P.253). Tap [Print]. UV-1900i Operation Guide...
  • Page 242 The results are printed on the printer. Name Description Graph The graph after calculation is printed. The date and time when measurement of the original data is Date and time finished are printed. Measurement Measurement parameters of the original data are printed. parameters UV-1900i Operation Guide...

  • Page 243: Data Processing Method

    14.5.1 Data Processing Method When Peak Pick is executed, the UV-1900i operates as follows: The program searches a section consisting of six continuously increasing data points (Section A) and a section consisting of six continuously decreasing data points (Section B), appearing after Section A.

  • Page 244: Performing Peak Pick

    14 Data Processing 14.5.2 Performing Peak Pick Tap [Peak Pick]. Peaks and valleys in the curve data are automatically detected. UV-1900i Operation Guide...
  • Page 245 "14.2 Operation of Common Buttons" P.197. to graph display Displays the curve. Graph Peaks or valleys in the curve are indicated by white cursors. Returns to the Operations screen. [Close] Tap this button to exit the screen. UV-1900i Operation Guide...
  • Page 246 Displays the horizontal values (wavelength or time) of peaks or Horizontal value valleys. The unit is [WL (nm)], [Time (min)], or [Time (s)]. Photometric Displays the photometric values of peaks or valleys. value The unit is [Abs], [%T], [%R], or [E]. UV-1900i Operation Guide...

  • Page 247: Printing The Results (Peak Pick)

    14.5 Peak Pick 14.5.3 Printing the Results (Peak Pick) The results of Peak Pick can be printed out. Grid type of the graph is selectable when using a commercially available printer ("15.13 Setting the Printer" P.253). Tap [Print]. UV-1900i Operation Guide...
  • Page 248 The date and time when measurement of the original data is Date and time finished are printed. Measurement Measurement parameters of the original data are printed. parameters Results of Peak Information about detected peaks and valleys is printed. Pick UV-1900i Operation Guide...

  • Page 249: Point Pick

    Displays a list of photometric values for up to 20 points at a specified interval from a specified abscissa (wavelength or time). Open the setting screen for Point Pick (Even Interval). Tap [Point Pick]. Tap [Even Intervals]. UV-1900i Operation Guide...
  • Page 250 [Execute] Executes Pick using set parameters. [Cancel] Returns to the Operations screen. Data is obtained according to [Data Interval] set in measurement parameters settings. For the start time and Interval, set an integer multiple of [Data Interval]. UV-1900i Operation Guide...
  • Page 251 The unit is [Abs], [%T], [%R], or [E]. Returns to the pick method selection screen. [Recalculate] Tap this button to perform recalculation after changing the parameters. Returns to the Operations screen. [Close] Tap this button to exit the screen. UV-1900i Operation Guide...

  • Page 252: Performing Point Pick (Arbitrary)

    14 Data Processing 14.6.2 Performing Point Pick (Arbitrary) Displays a list of photometric values for up to 20 points at arbitrary horizontal values (wavelength or time). Open the setting screen for Point Pick (Arbitrary). Tap [Point Pick]. Tap [Arbitrary]. UV-1900i Operation Guide...
  • Page 253 Returns to the Operations screen. Tap [Execute]. Specify data (wavelengths or time) of the points to be picked. The number of points is the number set in [Number of Picks]. "4.2.2 Numeric Input Screen (10-button Keypad)" P.29 UV-1900i Operation Guide...
  • Page 254 The unit is [Abs], [%T], [%R], or [E]. Returns to the pick method selection screen. [Recalculate] Tap this button to perform recalculation after changing the parameters. Returns to the Operations screen. [Close] Tap this button to exit the screen. UV-1900i Operation Guide...

  • Page 255: Printing The Results (Point Pick)

    14.6 Point Pick 14.6.3 Printing the Results (Point Pick) The results of Point Pick can be printed out. Grid type of the graph is selectable when using a commercially available printer ("15.13 Setting the Printer" P.253). Tap [Print]. UV-1900i Operation Guide...
  • Page 256 The date and time when measurement of the original data is Date and time finished are printed. Measurement Measurement parameters of the original data are printed. parameters Results of Point Information about picked points is printed. Pick UV-1900i Operation Guide...

  • Page 257: Peak Area

    The sign of a for the area protruding upwards is (+), and (-) for that protruding downwards. b is the area of the portion bounded by a straight line connecting the starting and ending points on the curve data and the horizontal axis. The sign of b is always (+). Example) UV-1900i Operation Guide...

  • Page 258: Performing Area Calculation

    The sign of a for the area protruding upwards is (-), and (+) for that protruding downwards. b is the area of the portion bounded by the curve data and horizontal axis (the starting and ending points) The sign of b is always (+). Example) 14.7.2 Performing Area Calculation Tap [Peak Area]. UV-1900i Operation Guide...
  • Page 259 Displays the photometric value at intersection of the curve and Photometric the cursor. value Absorbance at the start and end points is displayed. Executes area calculation according to the settings of start and [Execute] end points. [Cancel] Returns to the Operations screen. UV-1900i Operation Guide...
  • Page 260 Displays the results of calculation (values of a, b, and a + b). results Returns to the screen to set calculation parameters. [Recalculate] Tap this button to perform recalculation after changing the parameters. Returns to the Operations screen. [Close] Tap this button to exit the screen. UV-1900i Operation Guide...

  • Page 261: Printing The Results (Peak Area)

    14.7 Peak Area 14.7.3 Printing the Results (Peak Area) The results of peak area calculation can be printed out. Tap [Print]. Tap [Calculation data]. The results are printed on the printer. UV-1900i Operation Guide...
  • Page 262 Description Graph The graph of the original data is printed. The date and time when measurement of the original data is Date and time finished are printed. Measurement Measurement parameters of the original data are printed. parameters UV-1900i Operation Guide...

  • Page 263: Data Print

    14.8 Data Print Prints measurement parameters and data at each sampling point of curve data. • Printing cannot be terminated on the UV-1900i. To stop printing urgently, disconnect the USB cable connected between the UV-1900i and the printer. • The number of digits of printed data is in accordance with "15.11 Setting the...
  • Page 264 The graph is printed. Date and time The date and time of the end of the measurement are printed. Measurement Measurement parameters of the data are printed. parameters Sampling point Information about each sampling point is printed. information UV-1900i Operation Guide...

  • Page 265: Displaying The General Settings Menu

    • The parameters set in this menu are stored internally, even if the power is turned OFF. 15.1 Displaying the General Settings Menu Tap [General Settings]. The General Settings screen is displayed. The General Settings menu has five tabs. UV-1900i Operation Guide...
  • Page 266 [Display Off when no operation is performed for a certain period of time. Time] "15.4 Setting Display Off Time" P.243 Sets the buzzer. [Buzzer] "15.5 Setting the Buzzer" P.244 Sets the network. [Network] "15.6 Network Settings" P.245 UV-1900i Operation Guide...
  • Page 267 Exchanges the light flux on the sample side and reference (subject) side. "15.8 Setting S/R Exchange" P.248 [S/R Exchange] This function is used when a specular reflectance attachment (incidence angle 5°) is installed. Normally, do not change this setting. UV-1900i Operation Guide...
  • Page 268 Specifies the number of decimals of the photometric values to [Number of be displayed. Decimals] "15.11 Setting the Number of Decimals" P.251 Specifies the background color to display the curve data. [Graph BG Color] "15.12 Setting Graph Background Color" P.252 UV-1900i Operation Guide...
  • Page 269 Sets the printer to be used and the format of a hard copy. [Printer] "15.13 Setting the Printer" P.253 Sets the keyboard layout. [Keyboard layout] "15.14 Setting the Keyboard Layout" P.260 [Format Formats the expanded memory. Expanded "15.15 Setting the Expanded Memory" P.261 Memory] UV-1900i Operation Guide...
  • Page 270 [Save CSV File function is enabled in the Spectrum mode. During Auto File] "15.17 Setting of Save CSV During Auto File" P.266 Sets the automatic startup function of the instrument. [Wakeup] "15.18 Setting the Wakeup Function" P.267 UV-1900i Operation Guide...

  • Page 271: Setting The Light Source Lamp

    15.2 Setting the Light Source Lamp 15.2 Setting the Light Source Lamp The UV-1900i uses a deuterium lamp (D2 lamp) in the ultra-violet region and a halogen lamp (WI lamp) in the visible/near-infrared region. Select whether to automatically switch between the two light sources according to measurement wavelengths, or to fix them regardless of the wavelengths.

  • Page 272: Setting Lcd Brightness

    Display/Set Tap the input field to display numeric keypad ("4.2.2 Numeric button Input Screen (10-button Keypad)" P.29). Brightness Decreases the current brightness by one increment. Decrease button Brightness Increases the current brightness by one increment. Increase button UV-1900i Operation Guide...

  • Page 273: Setting Display Off Time

    When selecting [10 min], [30 min], or [60 min], the LCD backlight is automatically turned OFF if nothing is executed for the set time. When selecting [ ∞], the LCD backlight is not automatically turned OFF. Tap the screen to illuminate the backlight again. UV-1900i Operation Guide...

  • Page 274: Setting The Buzzer

    LED light at the bottom right of the touch panel illuminates in red even if the alarm is turned off. Specifies the volume of the buzzer. Tap to select [High], [Volume] [Middle] or [Low]. [Apply] Confirms changes and closes the window. UV-1900i Operation Guide...

  • Page 275: Network Settings

    Tap [Network]. Specifies the items as desired. Name Description Sets the IP address of the instrument. Tap the input field to display numeric keypad ("4.2.2 [IP Address] Numeric Input Screen (10-button Keypad)" P.29). The default value is [192.168.200.100]. UV-1900i Operation Guide...

  • Page 276: Setting The Accumulation Time

    Specifies the accumulation time to read data in the Photometric, Photometric 8l, Quantitation, and Bio-method modes. In the Spectrum mode, the accumulation time is determined by the setting of [Scan Speed] in the [Scan Settings] subtab ("[Scan Settings] subtab" P.73). Tap [Accumulation Time]. UV-1900i Operation Guide...
  • Page 277 The longer the time become, the less the dispersion of the obtained data, but the number of data outputs per unit time becomes less. The number of outputs per second is almost the reciprocal of the accumulation time. UV-1900i Operation Guide...

  • Page 278: Setting S/R Exchange

    When [Normal] is selected, the front of the sample compartment will be the sample light flux, and the back will be the reference light flux. When [Inverse] is selected, the front of the sample compartment will be the reference light flux, and the back will be the sample light flux. UV-1900i Operation Guide...

  • Page 279: Setting Date And Time

    Specifies the date print format. Tap to select [yyyy/mm/dd], [mm/dd/yyyy], or [dd/mm/yyyy]. [Format] The year, month, and day are printed in "yyyy", "mm", and "dd", respectively. [Apply] Confirms changes and closes the window. [Cancel] Discards changes and closes the window. UV-1900i Operation Guide...

  • Page 280: Setting The Language

    15 General Settings 15.10 Setting the Language Sets the language to be displayed. Tap [Language]. Tap the item to be set. [日本語](Japanese), [English], [ ](Chinese), [Español (México)](Spanish (Mexico)), [Português (Brasil)](Portuguese (Brazil)), [Русский](Russian), [Deutsch](Germany), and [Français](French) are available. UV-1900i Operation Guide...

  • Page 281: Setting The Number Of Decimals

    • Abs (Absorbance): Rounded to four decimal place (i.e. the fifth decimal place is rounded off). • %T (Transmittance) or %R (Reflectance): Rounded to two decimal places (i.e. the third decimal place is rounded off). • E (Energy): Rounded to two decimal places (i.e. the third decimal place is rounded off). UV-1900i Operation Guide...

  • Page 282: Setting Graph Background Color

    15 General Settings 15.12 Setting Graph Background Color Specifies the background color to display the curve data. Tap [Graph BG Color]. [Black] and [White] are available. Each tap of this key toggles the item. UV-1900i Operation Guide...

  • Page 283: Setting The Printer

    Specifies the printer type. For details, see "15.13.1 Selecting the [Printer Type] Printer" P.255. Each tap of this key toggles between ON and OFF of Date Printing. OFF: [Date Printing] When [Printer Type] (1) is set to [PictBridge], this setting is disabled. UV-1900i Operation Guide...
  • Page 284 • Printing of curve data in the Kinetics mode ("10.3.3 Manual Print (Curve data)" P.139) • Printing of curve data in the Kinetics Rate mode ("11.3.3 Manual Print (Curve data)" P.155) [Apply] Confirms changes and closes the window. [Cancel] Discards changes and closes the window. UV-1900i Operation Guide...

  • Page 285: Selecting The Printer

    To plug the USB printer cable to another port or computer after (or while) using ESC/P Raster (ESC/P-R) printer, be sure to turn OFF the printer power beforehand. If the USB cable is unplugged before the printer head returns to its home position, printing cannot be accepted. UV-1900i Operation Guide...

  • Page 286: Changing Printer Driver (Only When Esc/P-R Is Selected)

    "Changing printer driver" P.257. n Preparing the printer driver To learn about ESC/P-R printers compatible with UV-1900i, and how to obtain drivers specific to the printers, contact your Shimadzu Representative where you purchased the product. Prepare a USB memory, and create "(USB memory drive)\UV1900\Devinfo".
  • Page 287 15.13 Setting the Printer n Changing printer driver Connect a USB memory to the instrument. Open the setting screen for printer driver. Set [Printer Type] to [ESC/P-R]. Tap [Printer Driver]. Tap printer driver to be used. Tap [OK]. UV-1900i Operation Guide...

  • Page 288: Setting The Printer Name (Only When Selecting Pictbridge)

    Printers compatible with PictBridge are commercially available. Open the setting screen for the printer name. Set [Printer Type] to [PictBridge]. Tap [Printer name]. Tap the printer name to be used. Available printers are displayed in a list. UV-1900i Operation Guide...

  • Page 289: Changing The Paper Size (Only When Selecting Pictbridge)

    Tap [Apply]. The printer to be used is changed. 15.13.4 Changing the Paper Size (Only When Selecting PictBridge) Tap [Paper Size]. Tap the item to be set. The paper sizes unavailable for the printer are not displayed. UV-1900i Operation Guide...

  • Page 290: Setting The Keyboard Layout

    15 General Settings 15.14 Setting the Keyboard Layout Set the keyboard layout. Tap [keyboard layout]. Tap the item to be set. [Japanese], [English (U.S.)], [Chinese], [Spanish (Mexico)], [Portuguese (Brazil)], [Russian], [Germany], and [French] are available. UV-1900i Operation Guide...

  • Page 291: Setting The Expanded Memory

    15.15 Setting the Expanded Memory Format the expanded memory. If you perform formatting, all saved files will be deleted. Tap [Format Expanded Memory]. Tap [Yes]. Formatting of the expanded memory starts. Tap [OK]. The General Settings menu screen appears. UV-1900i Operation Guide...

  • Page 292: Setting The Start Program

    There are 3 pages of items. Tap to switch the pages. Start Program Description The Mode Menu screen ("3.1 Mode Menu Screen" P.18) will be [Standard Menu] displayed when the instrument starts up. This mode has been set as a factory default. UV-1900i Operation Guide...
  • Page 293 When selecting [Saved Params File], perform the following procedures to specify the file to be loaded at startup. Specifies the location of the file to be loaded. Tap the [Location] selection field. Tap [Built-in Memory], [USB Memory] or [Expanded Memory]. UV-1900i Operation Guide...
  • Page 294 • Tap [Close] to close the screen. [Close] Cancels file selection and returns to the General Settings menu. [Open] Specifies selected file as the file to be loaded at startup. UV-1900i Operation Guide...
  • Page 295 The item icon varies depending on the type of selected file. When a parameters file in the built-in memory is selected, is displayed. For a parameters file in the USB memory, and for a measurement parameters file in the expanded memory, UV-1900i Operation Guide...

  • Page 296: Setting Of Save Csv During Auto File

    (ex. the name of the CSV file saved with 001.spc is 001.csv). Tap [Save CSV During Auto File]. [ON] and [OFF] are available. Each tap of this key toggles the item. When [ON] is selected, CSV file saving is enabled. When [OFF] is selected, it is disabled. UV-1900i Operation Guide...

  • Page 297: Setting The Wakeup Function

    ("4.2.2 [Start Time] Numeric Input Screen (10-button Keypad)" P.29). The hour of the startup time can be set in the range of 0 to 23. The minutes can be set in the range of 0 to 59. UV-1900i Operation Guide...
  • Page 298 Also, if the date set in [Start on the Specified Day] is earlier than the current date, you cannot set the instrument to the sleep state. [Apply] Saves changes and closes the window. [Cancel] Discards changes and closes the window. UV-1900i Operation Guide...

  • Page 299: Maintenance

    Maintenance This section explains the functions used for maintenance and inspection of UV-1900i. This section explains the following functions. For the validation function, see "17 Validation Function" P.288. • "16.2 Wavelength Recalibration" P.271 • "16.3 Resetting Lamp Lighting Time" P.273 •...
  • Page 300 Sets security functions. You can switch ON/OFF each security function, change passwords, and set limitations for each [Security function. Settings] "16.4 Security Mode" P.274 Updates system software of the UV-1900i. [Update System Program] "16.5 Update System Program" P.286 UV-1900i Operation Guide...

  • Page 301: Wavelength Recalibration

    Calibrates the wavelength origin by referencing the emission line of the D2 (deuterium) lamp (656.1 nm). Perform the wavelength recalibration in the following cases: • To recalibrate the original wavelength accuracy of the UV-1900i after an extended period without powering on the instrument or to check instrument function.
  • Page 302 16 Maintenance Tap [Yes]. Wavelength recalibration starts. When the wavelength recalibration is completed, the Maintenance menu is displayed. UV-1900i Operation Guide...

  • Page 303: Resetting Lamp Lighting Time

    16.3 Resetting Lamp Lighting Time When halogen lamp (WI lamp) or deuterium lamp (D2 lamp) is replaced ("3.4 Replacing the Lamp" in "UV-1900i Instruction Manual (Installation and Maintenance Guide)", reset lamp lighting time as follows: Tap [Reset] for replaced lamp.

  • Page 304: Security Mode

    • "16.4.2 Setting the Password" P.276 • "16.4.3 Setting Function Availability" P.279 Security functions includes the setting to start the UV-1900i in the PC Control mode ("18 PC Control Mode" P.427). "16.4.4 Setting for Startup in PC Control Mode" P.284 16.4.1 Enabling Security Mode...
  • Page 305 • "16.4.2 Setting the Password" P.276 • "4.2.1 Text Input Screen (Keyboard)" P.27 If you forget the Administrator password, contact your Shimadzu representative. The default password is "Shimadzu". Tap [OK]. Tap [Security Mode] to turn it ON ( UV-1900i Operation Guide...

  • Page 306: Setting The Password

    Three types of user level are available: "Administrator" for the administrative manager, "Developer" for the inspection manager, and "Operator" for the operator of the UV-1900i. Enter the set password to log in to the UV-1900i ("2.2.1 Changing User Level and Login" P.9). The administrative manager should protect Administrator password by him/herself and inform the inspection manager about Developer password and the operator about Operator password.
  • Page 307 16.4 Security Mode Tap [OK]. Tap [Change Password]. Select the user level. Tap the [User] selection field. Tap the user level whose password is to be set. UV-1900i Operation Guide...
  • Page 308 Tap the input field to display text input screen ("4.2.1 Text Input Screen (Keyboard)" P.27). Tap the [New Password (re-enter)] entry field and enter the same password as previously entered. Tap [Apply]. The [Security Settings] screen is displayed. UV-1900i Operation Guide...

  • Page 309: Setting Function Availability

    Tap [Security Settings]. Tap the entry field. Enter Administrator Password. • "16.4.2 Setting the Password" P.276 • "4.2.1 Text Input Screen (Keyboard)" P.27 If you forget the Administrator password, contact your Shimadzu representative. The default password is "Shimadzu". UV-1900i Operation Guide...
  • Page 310 16 Maintenance Tap [OK]. Tap [Security Items Settings]. UV-1900i Operation Guide...
  • Page 311 • [Administrator + Developer + Operator] Tap the user level. Perform this procedure for each function. After setting, tap [Apply]. The [Security Settings] screen is displayed. If you tap [Cancel], the setting is not saved and the [Security Settings] screen appears. UV-1900i Operation Guide...
  • Page 312 The right to perform the data processing in the Spectrum and Time Course modes. [Operate Data] "14 Data Processing" P.195 The right to update the system program of the UV-1900i. [Update System Program] "16.5 Update System Program" P.286 UV-1900i Operation Guide...
  • Page 313 *1 The default authorities are shown. "A", "D", and "O" stand for Administrator, Developer, and Operator, respectively. The tick mark (P) means that corresponding user(s) can use the particular function. *2 Use this function to protect the status where LabSolutions UV-Vis software (standard accessory) controls the instrument history. UV-1900i Operation Guide...

  • Page 314: Setting For Startup In Pc Control Mode

    16 Maintenance 16.4.4 Setting for Startup in PC Control Mode Make the setting to start the UV-1900i in the PC Control mode ("18 PC Control Mode" P.427). Tap [Security Settings]. Tap the entry field. UV-1900i Operation Guide...
  • Page 315 • "16.4.2 Setting the Password" P.276 • "4.2.1 Text Input Screen (Keyboard)" P.27 If you forget the Administrator password, contact your Shimadzu representative. The default password is "Shimadzu". Tap [OK]. Tap [Start-up in PC Control]. Each tap of this key toggles between OFF (...

  • Page 316: Update System Program

    16 Maintenance 16.5 Update System Program The system program used for the UV-1900i should be manually updated. When system program update is required due to modifications or bug fix, update the program as follows: Do NOT turn "OFF" the power during update of the system programs.
  • Page 317 16.5 Update System Program Tap [Yes]. System program update starts. When a message saying the update is completed, tap [OK]. The UV-1900i restarts and the system program is updated. UV-1900i Operation Guide...

  • Page 318: Validation Function

    Once the tests started, all of selected tests are continuously conducted. The following shows operation flow. For details on each procedure, see reference for each procedure. Display the Validation menu. "17.2 Displaying the Validation Menu" P.295 Tap [Maintenance]. Tap [Validation]. The Validation menu is displayed. UV-1900i Operation Guide...
  • Page 319 The Test Item List (Parameters Settings) screen is displayed. Tap the checkbox on the left of the test to be conducted to place Specify parameters for the tests to be conducted. "17.4 Setting Validation Parameters" P.306 of the test to be conducted. UV-1900i Operation Guide...
  • Page 320 Repeat steps 1 to 3 to specify parameters for the tests to be conducted. When setting of all parameters is completed, tap on the Test Item List (Parameters Settings) screen. The screen returns to the Validation menu. UV-1900i Operation Guide...
  • Page 321 Set whether the Auto Print function and optional devices are used or not, etc. as needed. "17.5 Advanced Settings" P.353 Tap [Advanced]. Make necessary settings. When the setting is completed, tap [Apply]. The screen returns to the Validation menu. UV-1900i Operation Guide...
  • Page 322 USB memory or expanded memory. "17.6 Starting the Test" P.355 Tap [Start]. Make the setting for saving test results. Test results will be automatically saved according to this setting after the tests are completed. Tap [Go]. Selected tests are continuously conducted. UV-1900i Operation Guide...
  • Page 323 The Summary screen allows you to check the overview of the results of each test. If there is any failed item, the test item name will be displayed in red. Move to the tab including the test item to check, and tap Check the test results. UV-1900i Operation Guide...
  • Page 324 Save the results in a file or print them as needed. • "17.9.1 Saving Test Parameters File" P.396 • "17.9.4 Save Test Results as CSV" P.402 • "17.10 Printing Test Results" P.404 The above procedures are the operation flow in the Validation function. UV-1900i Operation Guide...

  • Page 325: Displaying The Validation Menu

    17.2 Displaying the Validation Menu 17.2 Displaying the Validation Menu The Validation function can be activated on the Validation menu under the Maintenance menu. Tap [Maintenance]. The Maintenance menu is displayed. Tap [Validation]. UV-1900i Operation Guide...
  • Page 326 "17.9.4 Save Test Results as CSV" P.402 Executes tests using set parameters. [Start] "17.6 Starting the Test" P.355 Loads saved test parameters. [Load Params] "17.9.2 Loading Test Parameters File" P.398 Saves set test parameters. [Save Params] "17.9.1 Saving Test Parameters File" P.396 UV-1900i Operation Guide...

  • Page 327: Selecting Tests To Be Conducted

    17.3 Selecting Tests to be Conducted 17.3 Selecting Tests to be Conducted Selects tests to be conducted on the Test Item List (Parameters Settings) screen. Tap [Parameters Settings] on the Validation menu. The Test Item List (Parameters Settings) screen is displayed. UV-1900i Operation Guide...
  • Page 328 Select tests to be conducted. Tap the checkbox on the left of each item to turn on/off . Place for tests to be conducted. Test items of each item to set test parameters. "17.4 Setting Validation Parameters" P.306 UV-1900i Operation Guide...
  • Page 329 Pharmacopoei (USP), respectively. • [Others] ([JP], [EP], or [USP] is not displayed) They are not tests defined in the Pharmacopoeia, but tests conducted by Shimadzu sales/service representative in periodical inspection. When the button is tapped, the following screen is displayed.

  • Page 330: Semi-Automatic Validation Items

    Repeatability Up to 4 types of filters can be used for the validation. For setting of test parameters, see "17.4.4 Setting - [JP] Photometric Repeatability" P.316. UV-1900i Operation Guide...
  • Page 331 Absorbance errors due to the stray light is described in the following reference. Refer to it when defining the criteria (pass/fail judgment value). • "6.3.7 Calibration Curve Curvature" in "UV-1900i Instruction Manual (Installation and Maintenance Guide)" • For setting of test parameters, see "17.4.5 Setting - Stray...
  • Page 332 (JQA), and other organizations evaluate and supply glass-optical filters and solution filters for calibrators of spectrometer transmittance (absorbance) and wavelength indication values. Various types of the filter are available depending on the wavelength and the type of vertical axis ranges to be calibrated. UV-1900i Operation Guide...
  • Page 333 *2 Prepare cells as many as the number of tested reagents. *3 Purchase P/N 200-34442 (10 mm square quartz cell). *4 Purchase P/N 200-34449 (5 mm path length quartz cell) and P/N 204-21473-02 (spacer for short path length cells: 1 pc). UV-1900i Operation Guide...

  • Page 334: Full-Automatic Validation Items

    If 1 hour has not elapsed after the startup initialization is completed when the validation starts, the Waiting screen is displayed. For setting of test parameters, see "17.4.18 Setting - Baseline Flatness" P.349. UV-1900i Operation Guide...
  • Page 335 Wavelengths (D2) For setting of test parameters, see "17.4.21 Setting - [USP] Control of Wavelengths (D2)" P.352. The RMS value is obtained using the following equation: Ai: Absorbance, Am: Mean value of absorbance, N: Number of data points UV-1900i Operation Guide...

  • Page 336: Setting Validation Parameters

    Tap it to turn on/off . Place to use the current tab execution setting for the validation. checkbox Subtab Switches setting items in the same tab. Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. UV-1900i Operation Guide...
  • Page 337 The last digit of the value is rounded off. Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 338 Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button Changes the wavelength value of selected item. Editing button Tap it to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...

  • Page 339: Setting - [Jp] Wavelength Repeatability

    Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Specifies the criteria used to evaluate the validation result. [Criteria] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 340 The last digit of the value is rounded off. Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 341 Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button Changes the wavelength value of selected item. Editing button Tap it to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...

  • Page 342: Setting - [Jp] Photometric Accuracy

    Subtab Switches setting items in the same tab. Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. UV-1900i Operation Guide...
  • Page 343 The last digit of the value is rounded off. Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 344 Selects the previous or next item. Check all button Checks ( ) all items. Clears check marks ( ) of all items. Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button UV-1900i Operation Guide...
  • Page 345 ±0.0043 Abs. Therefore, when checking whether the instrument photometric accuracy falls within ±0.004 Abs by using a 10 % transmittance filter, set the value in [Criteria] within ±0.008 Abs (the fourth decimal place is rounded off). UV-1900i Operation Guide...

  • Page 346: Setting - [Jp] Photometric Repeatability

    If the photometric type is changed after the value is entered, the set value is not converted, therefore, set the value again. Subtab Switches setting items in the same tab. Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. UV-1900i Operation Guide...
  • Page 347 The last digit of the value is rounded off. Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 348 Selects the previous or next item. Check all button Checks ( ) all items. Clears check marks ( ) of all items. Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button UV-1900i Operation Guide...
  • Page 349 • [Wavelength]: Enter the measurement wavelength. • [Criteria]: Enter the criteria used to evaluate the validation result. After entering a value on the numeric keypad, tap [Apply] on this screen. The entered value is reflected to the items in the WL List. UV-1900i Operation Guide...

  • Page 350: Setting - Stray Light

    Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. [Test Specifies the wavelength to measure the stray light. Wavelength] UV-1900i Operation Guide...
  • Page 351 Sodium iodine solution (10 g/l) 220.0 nm £ 0.05 %T NaNO2 Sodium nitrite solution (50 g/l) 340.0 nm Std Filter Other filter 370.0 nm These values are defined for the tests conducted by Shimadzu sales/service representative in periodical inspection. UV-1900i Operation Guide...

  • Page 352: Setting - [Ep] Control Of Wavelengths

    Input Screen (10-button Keypad)" P.29). Specifies the number of decimals of the values to be used for the validation. Tap it and select [1] or [2]. [Decimal Places] The last digit of the value is rounded off. UV-1900i Operation Guide...
  • Page 353 Name Description Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 354 Selects the previous or next item. Check all button Checks ( ) all items. Clears check marks ( ) of all items. Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button UV-1900i Operation Guide...
  • Page 355 • [Test WL]: Enter the measurement wavelength. • [Criteria]: Enter the tolerance used to evaluate the validation result. After entering a value on the numeric keypad, tap [Apply] on this screen. The entered value is reflected to the items in the WL List. UV-1900i Operation Guide...

  • Page 356: Setting - [Usp] Control Of Wavelengths

    Input Screen (10-button Keypad)" P.29). Specifies the number of decimals of the values to be used for the validation. Tap it and select [1] or [2]. [Decimal Places] The last digit of the value is rounded off. UV-1900i Operation Guide...
  • Page 357 Name Description Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 358 Selects the previous or next item. Check all button Checks ( ) all items. Clears check marks ( ) of all items. Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button UV-1900i Operation Guide...
  • Page 359 • [WL Precision Criteria]: Enter the pass/fail judgment criteria for variation of detected peak wavelengths. After entering a value on the numeric keypad, tap [Apply] on this screen. The entered value is reflected to the items in the WL List. UV-1900i Operation Guide...

  • Page 360: Setting - [Ep] Control Of Absorbance

    Input Screen (Keyboard)" P.27). Sets a certain serial number for the filter. Set serial number is printed as well as pass/fail results. [Serial number] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 361 The last digit of the value is rounded off. Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 362 Selects the previous or next item. Check all button Checks ( ) all items. Clears check marks ( ) of all items. Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button UV-1900i Operation Guide...
  • Page 363 [Wavelength]. • [Criteria]: Enter the criteria used to evaluate the validation result. After entering a value on the numeric keypad, tap [Apply] on this screen. The entered value is reflected to the items in the WL List. UV-1900i Operation Guide...

  • Page 364: Setting - [Usp] Control Of Absorbance

    Input Screen (Keyboard)" P.27). Sets a certain serial number for the filter. Set serial number is printed as well as pass/fail results. [Serial number] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 365 The last digit of the value is rounded off. Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.
  • Page 366 Selects the previous or next item. Check all button Checks ( ) all items. Clears check marks ( ) of all items. Clear all button Check/Clear Turn on/off the check mark ( ) of selected item. button UV-1900i Operation Guide...
  • Page 367 • [Abs Precision Criteria]: Enter the pass/fail judgment criteria for variation of measured values. After entering a value on the numeric keypad, tap [Apply] on this screen. The entered value is reflected to the items in the WL List. UV-1900i Operation Guide...

  • Page 368: Setting - [Ep] Limit Of Stray Light

    Tap it and select [1] or [2]. [Decimal Places] The last digit of the value is rounded off. Specifies the criteria used to evaluate the validation result. [Criteria] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). UV-1900i Operation Guide...
  • Page 369 Name Description Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.

  • Page 370: Setting - [Usp] Limit Of Stray Light

    [Test WL Range] respectively. Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. UV-1900i Operation Guide...
  • Page 371 Input Screen (10-button Keypad)" P.29). Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.

  • Page 372: Setting - [Ep] Resolution

    Input Screen (10-button Keypad)" P.29). Set the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.

  • Page 373: Setting - [Usp] Resolution

    Input Screen (10-button Keypad)" P.29). Sets the expiration date of the standard value of the filter. The UV-1900i automatically checks whether the filter has passed its expiration date when starting the validation, and if it is expired, [Filter Expiration the validation is cancelled.

  • Page 374: Setting - [Jp] Wavelength Accuracy (D2)

    • Specifies the criteria used to evaluate the validation result in [Criteria (nm)]. Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). [Recommended Set [Criteria (nm)] to ±0.3 nm. Value] This is the criteria required in the Japanese Pharmacopoeia. UV-1900i Operation Guide...

  • Page 375: Setting - [Jp] Wavelength Repeatability (D2)

    Tap the checkbox on the left of each line to turn on/off Place to use the wavelength of the line for the validation. [Recommended Set [Criteria] to ±0.2 nm. Value] This is the criteria required in the Japanese Pharmacopoeia. UV-1900i Operation Guide...

  • Page 376: Setting - Resolution (Spectral Bandwidth)

    Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. Set [Criteria] to £ 1.00 ± 0.20 nm. [Recommended These values are defined for the tests conducted by Shimadzu Value] sales/service representative in periodical inspection. UV-1900i Operation Guide...

  • Page 377: Setting - Noise Level

    Specifies the pass/fail judgment criteria used to evaluate the P-P value. [(P-P) Criteria] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. UV-1900i Operation Guide...
  • Page 378 [Recommended Value] £ 0.0006 £ 0.00010 500.0 nm 500.0 nm 340.0 nm 340.0 nm £ 0.0024 £ 0.00040 200.0 nm 200.0 nm These values are defined for the tests conducted by Shimadzu sales/service representative in periodical inspection. UV-1900i Operation Guide...

  • Page 379: Setting - Baseline Flatness

    • [Test WL Range] starting wavelength: 1100 nm [Recommended • [Test WL Range] ending wavelength: 190 nm Value] • [Criteria]: ±0.001 Abs These values are defined for the tests conducted by Shimadzu sales/service representative in periodical inspection. UV-1900i Operation Guide...

  • Page 380: Setting - Drift (Baseline Stability)

    Specifies the criteria used to evaluate the validation result. [Criteria] Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). Saves the setting and returns to the Test Item List (Parameters Back button Settings) screen. UV-1900i Operation Guide...

  • Page 381: Setting - [Ep] Control Of Wavelengths (D2)

    • [Test Wavelength] in the [340 nm] tab: 340 nm Value] • [Criteria]: £ 0.001 Abs/H These values are defined for the tests conducted by Shimadzu sales/service representative in periodical inspection. 17.4.20 Setting - [EP] Control of Wavelengths (D2) Name...

  • Page 382: Setting - [Usp] Control Of Wavelengths (D2)

    Tap the input field to display numeric keypad ("4.2.2 Numeric Input Screen (10-button Keypad)" P.29). [Recommended Set [Accuracy (nm)] to ±2.0 nm, and [Precision (nm)] to ±0.5 nm. Value] This is the criteria required in the United States Pharmacopoeia. UV-1900i Operation Guide...

  • Page 383: Advanced Settings

    ("17.10 Printing Test Results" P.404) are printed. Each tap of this key toggles between ON and OFF. OFF: [Record Initialization Results] For details on the initialization, refer to “2.5.2 Initialization Operation” in "UV-1900i Instruction Manual (Installation and Maintenance Guide)". UV-1900i Operation Guide...
  • Page 384 Confirms changes and closes the window. [Cancel] Discards changes and closes the window. Settings can be saved or loaded as a part of test parameters. • "17.9.1 Saving Test Parameters File" P.396 • "17.9.2 Loading Test Parameters File" P.398 UV-1900i Operation Guide...

  • Page 385: Starting The Test

    Input Screen (Keyboard)" P.27). Specifies the destination location of the file. Tap it to select [Location] [Built-in Memory], [USB Memory], or [Expanded Memory]. [Go] Confirms the setting and starts the test. [Cancel] Cancels the setting and closes the window. UV-1900i Operation Guide...
  • Page 386 "17.7 Operation During the Test" P.357 When all scheduled test items are completed, you will return to the Validation menu. Check and print the results. • "17.8 Checking Test Results" P.378 • "17.10 Printing Test Results" P.404 UV-1900i Operation Guide...

  • Page 387: Operation During The Test

    • When [Stop all remaining tests.] is tapped, all tests are canceled and you will return to the Validation menu. The results of tests completed before termination are saved, therefore, you can check or print them. UV-1900i Operation Guide...
  • Page 388 When [Check Results after Each Test] ("17.5 Advanced Settings" P.353) is enabled, the test is suspended to enable you to check results after each test. To resume the test, tap [To Next Test] on the bottom right of the screen. UV-1900i Operation Guide...
  • Page 389 • When [Stop all remaining tests.] is tapped, all tests are canceled and you will return to the Validation menu. The results of tests completed before termination are saved, therefore, you can check or print them. UV-1900i Operation Guide...

  • Page 390: Operation During The Test - [Jp] Wavelength Accuracy

    *1 Spectrum measurement is performed within the range of ±10 nm of the specified test wavelength. *2 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *3 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 391: Operation During The Test - [Jp] Wavelength Repeatability

    *1 Spectrum measurement is performed within the range of ±10 nm of the specified test wavelength. *2 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *3 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 392: Operation During The Test - [Jp] Photometric Accuracy

    ("Installation of the Filter" P.358). *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 393: Operation During The Test - [Jp] Photometric Repeatability

    When using the 6-position Multi-Cell or CPS series device, the filter cannot be removed nor inserted. *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 394: Operation During The Test - Stray Light

    ("Installation of the Filter" P.358). *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 395: Operation During The Test - [Ep] Control Of Wavelengths

    *1 Spectrum measurement is performed within the range of ±10 nm of the specified test wavelength. *2 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *3 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 396: Operation During The Test - [Ep] Control Of Absorbance

    ("Installation of the Filter" P.358). *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 397: Operation During The Test - [Usp] Control Of Absorbance

    When using the 6-position Multi-Cell or CPS series device, the filter cannot be removed nor inserted. *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 398: Operation During The Test - [Ep] Limit Of Stray Light

    ("Installation of the Filter" P.358). *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 399: Operation During The Test - [Usp] Limit Of Stray Light

    ("Installation of the Filter" P.358). *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 400: Operation During The Test - [Ep] Resolution

    *1 The range of test wavelength is from 275.0 nm to 265.0 nm. *2 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *3 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 401: Operation During The Test - [Usp] Resolution

    "17.7.13 Operation During the Test - [EP] Resolution" P.370 17.7.15 Operation During the Test - [JP] Wavelength Accuracy (D2) *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 402: Operation During The Test - [Jp] Wavelength Repeatability (D2)

    17 Validation Function 17.7.16 Operation During the Test - [JP] Wavelength Repeatability (D2) *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 403: Bandwidth)

    17.7 Operation During the Test 17.7.17 Operation During the Test - Resolution (Spectral Bandwidth) *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 404: Operation During The Test - Noise Level

    To perform tests using multiple test wavelengths, perform the following procedures for each test wavelength. *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 405: Operation During The Test - Baseline Flatness

    To start the test immediately, tap [Continue]. The tests are executed according to the following flow. *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 406: Operation During The Test - Drift (Baseline Stability)

    17.7.21 Operation During the Test - [EP] Control of Wavelengths (D2) Operation of the instrument during the test are the same as those during the test of [JP] Wavelength Accuracy (D2). "17.7.15 Operation During the Test - [JP] Wavelength Accuracy (D2)" P.371 UV-1900i Operation Guide...

  • Page 407: Operation During The Test - [Usp] Control Of Wavelengths (D2)

    17.7 Operation During the Test 17.7.22 Operation During the Test - [USP] Control of Wavelengths (D2) *1 Executed when [Auto Print] is enabled ("17.5 Advanced Settings" P.353). *2 Executed when [Check Results after Each Test] is enabled ("17.5 Advanced Settings" P.353). UV-1900i Operation Guide...

  • Page 408: Checking Test Results

    P.396) by the procedures described below. • Test results are automatically saved, however, you also can save them as a CSV format file as needed ("17.9.4 Save Test Results as CSV" P.402). Tap [Check Results] on the Validation menu. UV-1900i Operation Guide...
  • Page 409 Tap the tab to switch it. to display the results of the test. Test items "17.8.1 Check Results - [JP] Wavelength Accuracy" P.380 "17.8.21 Check Results - [USP] Control of Wavelengths (D2)" P.395 Page up/down Switches the page displaying test items. button UV-1900i Operation Guide...

  • Page 410: Check Results - [Jp] Wavelength Accuracy

    The four buttons on the right operate as follows: The number of • results and page up/down button Moves to the previous or next page. • Selects the previous or next item. UV-1900i Operation Guide...

  • Page 411: Check Results - [Jp] Wavelength Repeatability

    The four buttons on the right operate as follows: The number of • results and page up/down button Moves to the previous or next page (by 10 page). • Moves to the previous or next page (by 1 page). UV-1900i Operation Guide...

  • Page 412: Check Results - [Jp] Photometric Accuracy

    • [Diff. (Abs/%T)]: The value obtained by subtracting [Std (Abs/%T)] from [Meas. (Abs/%T)]. • [Criteria (Abs/%T)]: The value of set criteria. • [Result]: When [Diff. (Abs/%T)] is within [Criteria (Abs/%T)], [Pass] is displayed. If not, [Fail] is displayed. UV-1900i Operation Guide...

  • Page 413: Check Results - [Jp] Photometric Repeatability

    The four buttons on the right operate as follows: The number of • results and page up/down button Moves to the first or last page. • Moves to the previous or next page. UV-1900i Operation Guide...

  • Page 414: Checking Results - Stray Light

    (%T)], [Pass] is displayed. If not, [Fail] is displayed. 17.8.6 Check Results - [EP] Control of Wavelengths Test results are displayed in the same way as [JP] Wavelength Accuracy. "17.8.1 Check Results - [JP] Wavelength Accuracy" P.380 UV-1900i Operation Guide...

  • Page 415: Check Results - [Usp] Control Of Wavelengths

    The four buttons on the right operate as follows: The number of • results and page up/down button Moves to the previous or next page (by 10 page). • Moves to the previous or next page (by 1 page). UV-1900i Operation Guide...

  • Page 416: Check Results - [Ep] Control Of Absorbance

    The four buttons on the right operate as follows: The number of • results and page up/down button Moves to the first or last page. • Moves to the previous or next page. UV-1900i Operation Guide...

  • Page 417: Check Results - [Ep] Limit Of Stray Light

    • [Result]: When [Absorbance (Abs)] is more than [Criteria (Abs)], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 418: Check Results - [Usp] Limit Of Stray Light

    • [Result]: When [Absorbance (Abs)] is not less than [Criteria (Abs)], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 419: Check Results - [Ep] Resolution

    Moves to the first or last page. • Moves to the previous or next page. 17.8.13 Check Results - [USP] Resolution Test results are displayed in the same way as [EP] Resolution. "17.8.12 Check Results - [EP] Resolution" P.389 UV-1900i Operation Guide...

  • Page 420: Check Results - [Jp] Wavelength Accuracy (D2)

    • [Result]: When [Diff. (nm)] is within [Criteria (nm)], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 421: Check Results - [Jp] Wavelength Repeatability (D2)

    • [Result]: When [Max Diff.] is within [Criteria], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 422: Check Results - Resolution (Spectral Bandwidth)

    • [Result]: When [Bandwidth (nm)] is within [Criteria (nm)], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 423: Check Results - Noise Level

    (Abs)] are not greater than the corresponding [Criteria (Abs)], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 424: Check Results - Baseline Flatness

    • [Result]: When [Flatness (Abs)] is within [Criteria (Abs)], [Pass] is displayed. If not, [Fail] is displayed. Graph Display Displays the graph of test results. button Tap [Close] on the Graph screen to return to this screen. UV-1900i Operation Guide...

  • Page 425: Check Results - Drift (Baseline Stability)

    17.8.21 Check Results - [USP] Control of Wavelengths (D2) Test results are displayed in the same way as [USP] Control of Wavelengths. However, data only at 486.0 nm and 656.1 nm is displayed. "17.8.7 Check Results - [USP] Control of Wavelengths" P.385 UV-1900i Operation Guide...

  • Page 426: Saving/Loading Test Files

    • To load saved files, tap [Load Params] or [Load Res.]. 17.9.1 Saving Test Parameters File Test Parameters File includes which test is set to be executed, the setting of each test, and advanced settings. Tap [Save Params] on the Validation menu. UV-1900i Operation Guide...
  • Page 427 Specify the name of the file to be saved. [File Name] Tap the input field to display text input screen ("4.2.1 Text Input Screen (Keyboard)" P.27). [Save] Saves the file and closes the window. [Close] Stops saving the file and closes the window. UV-1900i Operation Guide...

  • Page 428: Loading Test Parameters File

    The four buttons on the right operate as follows: The number of • results and selection/page Moves to the previous or next page when the list continues button for more than one page. • Selects the previous or next file. UV-1900i Operation Guide...
  • Page 429 Delete button Deletes the selected file. [Close] Closes the window. Loads the file selected in the File List and returns to the [Open] Validation menu. The test parameters are overwritten by loaded file. UV-1900i Operation Guide...

  • Page 430: Loading Test Results File

    The four buttons on the right operate as follows: The number of • results and selection/page Moves to the previous or next page when the list continues button for more than one page. • Selects the previous or next file. UV-1900i Operation Guide...
  • Page 431 Delete button Deletes the selected file. [Close] Closes the window. Loads the file selected in the File List and returns to the [Open] Validation menu. The test results are overwritten by loaded file. UV-1900i Operation Guide...

  • Page 432: Save Test Results As Csv

    Tap [Save Test Results as CSV] on the Validation menu. (Only when the expanded memory is used) Tap the destination. A CSV format file is saved in the following directory in the USB memory or expanded memory. \UV1900\ValResult UV-1900i Operation Guide...

  • Page 433: Executed Test List

    17.9 Saving/Loading Test Files 17.9.5 Executed Test List Displays a list of the executed tests. The items checked on the Test Item List (Parameters Settings) screen are displayed in a list. UV-1900i Operation Guide...

  • Page 434: Printing Test Results

    For examples of print format, see "17.10.3 Examples of Test Result Printing" P.406. 17.10.1 Test Data Print (Each Test) You can print test results while the test results are displayed. Tap [Print]. Tap [Test data]. The test results are printed. UV-1900i Operation Guide...

  • Page 435: Test Data Print (All Data)

    The latest results of each test are printed. When the test file is loaded after the latest test ("17.9.2 Loading Test Parameters File" P.398), the loaded test results are printed. Tap [Print]. Tap [All test data]. All test results are printed. UV-1900i Operation Guide...

  • Page 436: Examples Of Test Result Printing

    Set test parameters are printed. The test results are printed. Test Results When "Diff/nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 437 Set test parameters are printed. The test results are printed. Test Results When "MD/nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 438 The date and time when the test is executed are printed. Test parameters Set test parameters are printed. The test results are printed. Test Results When "Diff./XX" is within the range of "Criteria/XX", "Pass" is printed. If not, ">>Fail<<" is printed. UV-1900i Operation Guide...
  • Page 439 The date and time when the test is executed are printed. Test parameters Set test parameters are printed. The test results are printed. Test Results When "MD" is within the range of "Criteria", "Pass" is printed. If not, ">>Fail<<" is printed. UV-1900i Operation Guide...
  • Page 440 The date and time when the test is executed are printed. Test parameters Set test parameters are printed. The test results are printed. Test Results When "Stray/%T" is not greater than "Criteria/%T", "Pass" is printed. If not, ">>Fail<<" is printed. UV-1900i Operation Guide...
  • Page 441 Set test parameters are printed. The test results are printed. Test Results When "Diff./nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 442 When "WL Accuracy" is within the range of "WL Accuracy Test Results Criteria" and "WL Precision" is within the range of "WL Precision Criteria", "Pass" is displayed. If not, ">>Fail<<" is displayed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 443 The date and time when the test is executed are printed. Test parameters Set test parameters are printed. The test results are printed. Test Results When "Diff./nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. UV-1900i Operation Guide...
  • Page 444 The test results are printed. When "Abs Accuracy" is within the range of "Abs Accuracy Test Results Criteria" and "Abs Precision" is within the range of "Abs Precision Criteria", "Pass" is displayed. If not, ">>Fail<<" is displayed. UV-1900i Operation Guide...
  • Page 445 The test results are printed. Test Results When "Stray Light = XX Abs (at 198 nm)" is larger than "Criteria / Abs", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 446 Set test parameters are printed. The test results are printed. Test Results When "Stray Light = XX Abs" is larger than "Criteria / Abs", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 447 Test parameters Set test parameters are printed. The test results are printed. Test Results When "Ratio" is not less than "Criteria", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 448 Test parameters Set test parameters are printed. The test results are printed. Test Results When "Ratio" is not less than "Criteria", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 449 The date and time when the test is executed are printed. The test results are printed. Test Results When "Diff./nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 450 Criteria Set criteria are printed. The test results are printed. Test Results When "MD/nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 451 Set criteria are printed. The test results are printed. Test Results When "Resolution" is within the range of "Criteria / nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 452 The test results are printed. When values of two "Noise Level" are not greater than the Test Results corresponding values of "Criteria", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 453 Set test parameters are printed. The test results are printed. Test Results When "Baseline Flatness" is within the range of "Criteria / Abs", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 454 Set test parameters are printed. The test results are printed. Test Results When "Drift" is not greater than "Criteria / Abs/H", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 455 The date and time when the test is executed are printed. The test results are printed. Test Results When "Diff./nm" is within the range of "Criteria/nm", "Pass" is printed. If not, ">>Fail<<" is printed. Graph The graph of the test results is printed. UV-1900i Operation Guide...
  • Page 456 When "WL Accuracy" is within the range of "WL Accuracy Test Results Criteria" and "WL Precision" is within the range of "WL Precision Criteria", "Pass" is displayed. If not, ">>Fail<<" is displayed. Graph The graph of the test results is printed. UV-1900i Operation Guide...

  • Page 457: Preparation For Using Pc Control Mode

    USB cable. If the USB Driver for UV-1900 Series is installed, the computer recognizes the UV-1900i as a COM port device. To control the UV-1900i, you need to refer to the COM port number. Check it on the computer.
  • Page 458 The following image is an example when the disk drive is E drive. The directory of the disk drive differs depending on the setting of the computer. Double-click [CP210xVCPInstaller_x64.exe] when the OS of the PC is 64-bit Windows, and [CP210xVCPInstaller_x86.exe] when the OS is 32-bit Windows. Click [Yes]. Click [Next]. UV-1900i Operation Guide...
  • Page 459 Installation of the USB Driver for UV-1900 Series starts. After installation is completed, click [Finish]. Connect the USB connector on the left side of the instrument and the USB connector of the PC using a USB cable. UV-1900i Operation Guide...
  • Page 460 Log in as a user with Administrator privileges. When the Security Mode is enabled, only the user with Administrator privileges can enable/disable the PC Control Mode. Tap [PC Control]. The instrument enters the PC Control Mode. Right-click on the Desktop. UV-1900i Operation Guide...
  • Page 461 Make a note of the last number (n part) of "Silicon Labs CP210x USB to UART Bridge (COMn)". Preparation for using PC Control Mode is completed. Depending on the control method to be used, see below. • "18.2 Controlling with LabSolutions UV-Vis" P.432 • "18.3 Controlling with External Commands" P.432 UV-1900i Operation Guide...

  • Page 462: Controlling With Labsolutions Uv-Vis

    Instruction Manual Basic Operation Guide" saved in the supplied LabSolutions UV-Vis installation disk. 18.3 Controlling with External Commands You can also control the UV-1900i from the computer using a control program besides the LabSolutions UV-Vis software. 18.3.1 Switching to the PC Control Mode To control the instrument with external commands, switch the instrument into the PC Control mode.

  • Page 463: Control Codes (Commercially Available Printers) And Commands

    (Null) Slave data, etc. This is also called the terminator. n COM port transmission settings • Transfer rate: 128000 bps • Data bit: 7 bits • Stop bit: 1 bit • Parity bit: Odd UV-1900i Operation Guide...
  • Page 464 18 PC Control Mode n Commands The types of commands sent from the PC to the UV-1900i can be generally classified as the following according to the direction of the data flow. • Write command: Sets the status of the UV-1900i.

  • Page 465: Example Of Programming

    Before transmitting a command, the master (PC in this example) issues the ENQ code to the slave (the UV-1900i in this example) to prompt it to receive the command data. The slave returns ACK to notify the master that it is ready to receive the command data.
  • Page 466 18 PC Control Mode Ending: 3 in flowchart 1 When the master (the UV-1900i in this example) finishes processing the command data, it transmits EOT to the slave (PC in this example). The slave waits until EOT is transmitted, and then returns ACK to end the communication.
  • Page 467 This is the same as in protocol type A. Reception of answered-back data: 1 in flowchart 2 The master (the UV-1900i in this example) processes the command data. When the master finishes this processing, it issues the ENQ code before sending data to prompt the slave (PC in this example) to receive the data.
  • Page 468 18 PC Control Mode Flowchart 2 Check of the number of repetitions and time out is omitted. UV-1900i Operation Guide...

  • Page 469: Explanation Of Commands And Data

    Data The data sent from the UV-1900i is comprised of the following elements: "data code", "parameter", and "terminator". • A data code consists of a single lower-case alphabetic character. The code is the same as the command code.

  • Page 470: Programming With Visual Basic 2015

    • This chapter assumes readers have basic knowledge of Visual Basic 2015, and use Visual Basic 2015 as their development environment. • Note that the UV-1900i cannot be controlled from the SerialPort class of .Net Framework. Define the API functions.
  • Page 471 18.3 Controlling with External Commands Use the API functions. You can externally control the UV-1900i using the defined API functions as follows: Starting communication Start communication using the CreateFile function. Example) Start communication through the COM1 port. Dim hCom As IntPtr Dim strComPort As String strComPort = "COM1"...
  • Page 472 18 PC Control Mode Receiving data Receive data from the UV-1900i using the ReadFile function. Example) Receive 1 character. Dim bRet As Boolean Dim byBuffer()As Byte Dim nBytesRead As Int32 'Receive data: bRet = ReadFile( hCom, byBuffer, 1, nBytesRead, IntPtr.Zero ) Ending communication End the communication using the CloseHandle function.

  • Page 473: Command List

    This section shows a list of commands to be used. The terminator symbol has been omitted from the "command format" columns in the table. When sending to the UV-1900i, send a terminator code (NUL) appearing right after the content shown in the table as the actual command.
  • Page 474 • n = 0: Survey Scan Speed • n = 1: Fast (default value) • n = 2: Medium • n = 3: Slow • n = 4: Very Slow UV-1900i Operation Guide...
  • Page 475 • n = 1: ON Controls the ON/OFF of the D2 light source lamp. The parameter n corresponds with the D2 lamp lamp illumination status as shown below. ON/OFF • n = 0: OFF • n = 1: ON UV-1900i Operation Guide...
  • Page 476 Retrieves data which have been stored in the memory of the UV-1900i by the measurement command a. The parameter n is the number of data points to be retrieved, and allows you to retrieve n pieces of data from the start of the file (in the case of a spectrum, from the long wavelength end).
  • Page 477 0 £ z £ 1000 (´ 0.01 ml) Controls the ON/OFF of the syringe sipper indication lamp. This command is valid only if the syringe sipper is connected to the UV-1900i sample compartment. Syringe sipper The parameter n corresponds with the lamp lamp ON/OFF illumination status as shown below.
  • Page 478 Moves the cell position of the Multi-Cell, Micro Multi-Cell or CPS series cell. This command is valid only if a Multi-Cell, Micro Multi-Cell or CPS series cell is connected to the UV-1900i sample compartment. Move cell The parameter n corresponds with the position direction of cell movement as shown below.

  • Page 479: Setting Attachments

    In the [Attachments] tab of each measurement mode, you can make settings to use optional devices (such as multi-cell holder and sipper) as sample compartment. Refer to "4 Replacing the Sample Compartment Parts" in "UV-1900i Instruction Manual (Installation and Maintenance Guide)" for installation/removal of optional devices.
  • Page 480 160L/C/U/T "19.3 [Sipper 160] Settings" P.457 The setting to use the Syringe Sipper that performs measurements while drawing sample into a flow Syringe Sipper [Syringe Sipper] cell using a syringe pump. N/CN "19.4 [Syringe Sipper] Settings" P.459 UV-1900i Operation Guide...

  • Page 481: Settings Of [Multi-Cell (6 Cells)], [Mmc (8 Cells)], [Mmc (16 Cells)], And [Cps]

    Name Description Detects the origin of the cell position. To use the 6-position multi-cell holder and MMC-1600, install them on the UV-1900i, and then tap this button to initialize them. When initialization is not executed, [Not Initialized] is displayed on the right of the button. When initialize is executed, [Initialized] is displayed.
  • Page 482 Displays a list of values obtained in blank correction. Blank correction preview button "Blank correction preview screen" P.453 Starts measurement using the settings in this [Attachments] tab [Start] and other tabs. After the setting is completed, set other measurement conditions and start measurement. UV-1900i Operation Guide...
  • Page 483 • Switches displayed contents using the buttons on the right. Tap buttons to page up/down and buttons to move one line up/down. • Tap [Close] to return to the previous screen. UV-1900i Operation Guide...

  • Page 484: Sequential Measurement And Limitation

    However, whether the sequential measurement is available or not, the limitation on the functions depend on the measurement mode as shown below. To connect the CPS series to the UV-1900i, a USB adaptor for CPS (P/N 206-25234-91) is separately required.

  • Page 485: Reagent Blank Correction And Cell Blank Correction

    1 is canceled (subtraction or division) from each measured value. Because of this, even if time changes occur in the blank sample or drift develops due to the increasing temperature of the instrument, accurate data can be acquired by canceling these fluctuating factors. UV-1900i Operation Guide...
  • Page 486 If both blank correction methods are enabled, the cell blank correction is executed for each cell, and the reagent blank correction is executed using the Cell 1 sample value as a reagent blank on the measured values of the other cell positions. UV-1900i Operation Guide...

  • Page 487: Sipper 160] Settings

    The settings to use the Sipper 160. When using the auto sample changer ASC-5 (optional) for link measurement, refer to the manual for ASC-5. To connect the ASC-5 to the UV-1900i, a USB adaptor for ASC (P/N 206-25235-91) is separately required. Make necessary settings changes.
  • Page 488 Performs measurement without Sipper 160. To perform measurement in conjunction with Sipper 160, tap [Start] [Auto Meas.] or [Rinse] (8) or press the lever of Sipper 160. After the setting is completed, set other measurement conditions and start measurement. UV-1900i Operation Guide...

  • Page 489: Syringe Sipper] Settings

    [Rinse] For details, see "19.5.1 Operation of [Auto Meas.] or [Rinse]" P.461. Sets the pump rotation speed. Tap to select [0.1 ml/s], [0.2 ml/s], [Pump Speed] [0.3 ml/s], [0.6 ml/s], or [12 ml/s]. UV-1900i Operation Guide...
  • Page 490 Performs measurement without the Syringe Sipper. To perform measurement in conjunction with the Syringe [Start] Sipper, tap [Auto Meas.] or [Rinse] (5) or press the lever of the Syringe Sipper. After the setting is completed, set other measurement conditions and start measurement. UV-1900i Operation Guide...

  • Page 491: Supplementary Explanation On Operation (Common To The Sipper 160 And Syringe Sipper)

    "The number of executed rinses" displayed on the button is also updated. If the lever is pressed when [Rinses] is set to 2 or more, the screen to select [Manual Rinse] or [Sequential Rinse] does not appear and [Manual Rinse] is automatically selected. UV-1900i Operation Guide...

  • Page 492: Operation Of [Auto Zero] And [Base Corr.]

    Tap [Auto Zero With Sipping] to execute sipping, Auto Zero, and purging sequentially. • When [Base Corr.] is tapped Tap [Base Corr. Without Sipping] to execute normal Baseline Correction. Tap [Base Corr. With Sipping] to execute sipping, Baseline Correction, and purging sequentially. UV-1900i Operation Guide...

  • Page 493: Notes For Interlocking Asc-5 In Quantitation Mode

    When purge is completed for the number of standard samples, press "STOP" button on the ASC-5. The ASC-5 operation stops temporarily. While [Start] is displayed, press "Start" button on the ASC-5. Unknown sample measurement starts. Tap [Calib. Curve]. UV-1900i Operation Guide...

  • Page 494: Index

    Logout Data Processing Lowry Method 175, 183 Date and Time Derivative Derivative Quantitation Maintenance Derivative quantitation method Manual Print deuterium lamp 20, 241, 273 Maximum Display Off Time Measured data Measuring Multi-cell Continuously DNA Quantitation 175, 178 UV-1900i Operation Guide...
  • Page 495 20, 241, 273 Resolution 302, 303, 304, 342, 343, 346 S/R Exchange Save Curve Save Params 34, 396 Save Table Save Test Results as CSV Saving Saving an Image Saving File Saving Files Screen Shot Security Mode semi-automatic validation UV-1900i Operation Guide...
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