Shimadzu UV-2450 Instruction Manual
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Shimadzu UV-2450 Instruction Manual

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SPECTROPHOTOMETER
Read the instruction manual thoroughly before you use the product.
Keep this instruction manual for future reference.
INSTRUCTION MANUAL
User's System Guide
UV-2450
UV-2550
206-94649D
Sep. 2008

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Summary of Contents for Shimadzu UV-2450

  • Page 1 206-94649D Sep. 2008 INSTRUCTION MANUAL User's System Guide SPECTROPHOTOMETER UV-2450 UV-2550 Read the instruction manual thoroughly before you use the product. Keep this instruction manual for future reference.
  • Page 2 • Shimadzu Corporation provides this publication "as is" without warranty of any kind, either express or implied, including, but not limited to the implied warranties of merchantability or fitness for a particular purpose. • All rights are reserved, including those to reproduce this publication or parts thereof in any form without permission in writing from Shimadzu Corporation.
  • Page 3 • Follow the procedures described in the user's manual. • Observe all warnings and cautions. • Do not disassemble or modify the unit without the express approval of authorized Shimadzu Representative. Failure to do so may lead to dangerous situations or damage of the unit.
  • Page 4 In case of electrical failure, perform the following operations: 1. Press the " " side of the power switch located on the left side bottom of the UV2450/2550 to turn OFF the instrument. 2. After the power comes back on, start up the UV-2450/2550. UV-2450/2550...
  • Page 5 Risk of burn. Before replacing the lamp, turn the power switch off and allow the lamp to cool down. Warning label U V - 2 4 5 Fig. 1 UV-2450 Warning label U V - 2 5 5 Fig. 2 UV-2550...
  • Page 6 WARNING RISK OF ELECTRIC SHOCK Before replacing the fuses or changing the power source voltage, read Instruction Manual. Warning label Fig. 3 UV-2450 Warning label Fig. 4 UV-2550 UV-2450/2550...
  • Page 7 Symbols Indicated on the Unit Symbol Contents Indicates current (a. c.). Indicates protective conductor terminal. Indicates power ON. Indicates power OFF. UV-2450/2550...
  • Page 8 : UV-Visible Spectrophotometer/Visible Spectrophotometer Model Name : UV-2450 UV-2550 Manufacturer : SHIMADZU CORPORATION ANALYTICAL & MEASURING INSTRUMENTS DIVISION Address : 1, NISHINOKYO-KUWABARACHO, NAKAGYO-KU, KYOTO, 604-8511, JAPAN Authorized Representative in EU: Shimadzu Europa GmbH Address : Albert-Hahn-Strasse 6-10, 47269 Duisburg, F.R. Germany UV-2450/2550...
  • Page 9 They will advise you regarding the equipment take-back. WEEE Mark With your co-operation we are aiming to reduce contamination from waste electronic and electrical equipment and preserve natural resource through re-use and recycling. Do not hesitate to ask Shimadzu service representative, if you require further information. UV-2450/2550...
  • Page 10 This page is intentionally left blank. viii UV-2450/2550...

  • Page 11: Table Of Contents

    Communications and Printer Setup ....................2-10 System Initialization ......................... 2-11 Electro Magnetic Compatibility ......................2-13 Chapter 3 Construction UV-2450/2550 Photometer Unit ......................3-2 Sample Compartment of UV-2450/2550 Photometer Unit..............3-4 Photometric System .......................... 3-5 3.3.1 Optical System (UV-2450) ....................3-5 3.3.2 Optical System (UV-2550) ....................
  • Page 12 This page is intentionally left blank. UV-2450/2550...

  • Page 13: Chapter 1 Inspection

    1 Inspection Chapter 1 Inspection CONTENTS Inspection ............................1-2 UV-2450/2550...

  • Page 14: Inspection

    Inspection The UV-2450/2550 Spectrophotometer System consists of the photometer unit and its accessories, including separate computer and software. Upon unpacking, confirm that all parts and standard accessories listed below are included in your shipment. Table 1.1 Shipment Inspection Items Description...

  • Page 15: Chapter 2 Installation

    Personal Computer Hardware and Software Requirements..........2-4 2.1.5 Cable Connections ....................... 2-4 Software Installation .......................... 2-6 2.2.1 Installing UVProbe ........................ 2-6 2.2.2 Uninstalling UVProbe......................2-7 2.2.3 Application Mode ........................2-7 Communications and Printer Setup ....................2-10 System Initialization ......................... 2-11 Electro Magnetic Compatibility ......................2-13 UV-2450/2550...

  • Page 16: Hardware Installation

    Hardware Installation This chapter provides step-by-step instructions for setting up your UV-2450/2550 instrument and the software that comes with it. 2.1.1 Site Requirements An installation site measuring at least: (Width) 1120 mm (44'') (Depth) 710 mm (28'') (Height) 520 mm (20.5'') Should be made available for the UV-2450/2550 Spectrophotometer system.

  • Page 17: Instrument Voltage Setting

    Before changing a fuse or the inlet voltage, turn off the power switch and disconnect the power cable. CAUTION Use a 5A fuse for 100 - 120V setting, and a 3.15A fuse for 220 - 240V power voltage settings. UV-2450/2550...

  • Page 18: Personal Computer Hardware And Software Requirements

    2.1 Hardware Installation 2.1.4 Personal Computer Hardware and Software Requirements In addition to the UV-2450/2550 photometer unit, UVProbe software, and standard accessories, the following hardware and software are either required (and therefore provided with the complete system), recommended or useful.
  • Page 19 Mouse Port Asynchronous Serial port (COM1) to Port (LPT1) to photometer, Connect 9-pin connector end printer/plotter. of 12" adapter cable here, and 9-pin female connector end of adapter to corresponding connector of photometer. Fig. 2.1 Personal Computer (Rear view) (example) UV-2450/2550...

  • Page 20: Software Installation

    Software Installation 2.2.1 Installing UVProbe Install UVProbe as follows: Install Shimadzu Authentication Tool and UVProbe following the procedures from Step 1 to Step 2. Step 1 - Installing Shimadzu User Authentication Tool Click Shimadzu User Authentication Tool. Welcome dialog box appears. Read the description well and click the Next button.

  • Page 21: Uninstalling Uvprobe

    Click OK when the process has finished. 2.2.3 Application Mode Application modes are provided in three types: Normal, Security, and GLP. The available functions differ depending on each mode. Function Normal Security GLP function Security function Disabled Enabled Enabled GLP function Disabled Disabled Enabled UV-2450/2550...
  • Page 22 • A User Entry cannot be chosen as a data acquisition method of a sample table. • Saving at the existing file name is prohibited. • After Saved file, it cannot change the factor on a standard table and a sample table. UV-2450/2550...
  • Page 23 Enter "admin" for User ID and click the OK button. NOTE It is not possible to change from the other mode to the GLP mode and vice versa. In this case, UVProbe must be reinstalled after it is uninstalled once. UV-2450/2550...

  • Page 24: Communications And Printer Setup

    For usage of the printer, refer to the instruction manual provided with the printer. Next, it is necessary to connect your instrument with the PC. Refer to [Communicating with the Spectrophotometer] in UVProbe Tutorial and register your instrument to the UVProbe. 2-10 UV-2450/2550...
  • Page 25 ON. The entire procedure takes about 4 minutes for UV-2450 and 5 minutes for UV-2550. A window showing the progress of the initialization will appear on the screen. Next to each item listed in the window to be initialized is a status circle showing whether the item is being tested (yellow), whether it has passed (green) or failed (red).
  • Page 26 In "RAM Memory Check" to be performed at initialization just after turning ON the power, if the power is turned ON just after turning OFF the power, an error may not occur even if the battery that backs up the contents of RAM is abnormal. 2-12 UV-2450/2550...

  • Page 27: Electro Magnetic Compatibility

    Before touching the instrument, discharge the electro statics charged in operator's body to ground by touching metallic structure connected to ground. Do not install this instrument in such environment where strong electro magnetic fields are generated near by. UV-2450/2550 2-13...
  • Page 28 2.5 Electro Magnetic Compatibility This page is intentionally left blank. 2-14 UV-2450/2550...

  • Page 29: Chapter 3 Construction

    3 Construction Chapter 3 Construction CONTENTS UV-2450/2550 Photometer Unit ......................3-2 Sample Compartment of UV-2450/2550 Photometer Unit..............3-4 Photometric System .......................... 3-5 3.3.1 Optical System (UV-2450) ....................3-5 3.3.2 Optical System (UV-2550) ....................3-7 3.3.3 Cell holder / Light Beam Relative Position................3-9 3.3.4...

  • Page 30: Uv-2450/2550 Photometer Unit

    Grounding terminal To be used when the employed switch board has no grounding terminal. Use this terminal as a common ground for the UV-2450/ 2550 and other device(s). AC power receptacle (inlet) For supplying AC power to the photometer unit.
  • Page 31 3.1 UV-2450/2550 Photometer Unit Front View Right View Left View Fig. 3.1 Exterior Views of UV-2450/2550 Photometer UV-2450/2550...

  • Page 32: Sample Compartment Of Uv-2450/2550 Photometer Unit

    Sample Compartment of UV-2450/2550 Photometer Unit Fig. 3.2 UV-2450/2550 Sample Compartment Description Cell holder for reference beam Cell holder for sample beam Set screws fixing the cell holder UV-2450/2550...

  • Page 33: Photometric System

    M1 - M10: Mirrors Photomultiplier Fig. 3.3 Optical Schematics of UV-2450 The light emitted from the light source (deuterium lamp, D2 or halogen lamp, Wl) is reflected by the mirrors M1 and M2 and projected onto the monochromator. The light source is switched automatically...
  • Page 34 All the optical elements except light source are sealed from the atmosphere by the windows W, to keep them dust-free. This is one of the features of the UV-2450. The slit width is selected in 6 steps; 0.1, 0.2, 0.5, 1, 2, 5.

  • Page 35: Optical System (Uv-2550)

    The light emitted from the light source (deuterium lamp D2, or halogen lamp Wl) is reflected by the mirrors M1 and M2 and projected onto the pre-monochromator. The light source is switched automatically depending upon the wavelength as follows: UV-2450/2550...
  • Page 36 The pre-monochromator is composed of S1 (entrance slit), M3 (mirror), G1 (Grating), and S2 (exit slit). The grating is Shimadzu double blazed holographic, which ensures ultra low stray light. The main monochromator is composed of S3 (entrance slit), M4 (mirror), G2 (grating), M5 (mirror) and S3 (exit slit).

  • Page 37: Cell Holder / Light Beam Relative Position

    Fig. 3.6 Fig. 3.7 show the electrical system for the UV-2450 and UV-2550 respectively. The micro-computer CPU in the spectrophotometer works to control the light source lighting, light source switching, filter switching, slit width selection, grating switching (UV-2550 only), and wavelength scanning pulse motor, according to the commands from the data station.
  • Page 38 (T%), to perform baseline correction, logarithmic conversion (into Abs.) and smoothing. -HV circuit Addition circuit Reference cell Wl lamp Monochromator − Sample cell Reference D2 lamp 1signal Preamp. − Sample signal Dark-current signal A/D converter AT Compatible Personal Computer Printer Keyboard Fig. 3.6 Electrical System of UV-2450 3-10 UV-2450/2550...
  • Page 39 3.3 Photometric System -HV circuit Addition circuit Reference cell Wl lamp Monochromator − D2 lamp Sample cell Reference 1signal Preamp. − Sample signal Dark-current signal A/D converter AT Compatible Personal Computer Printer Keyboard Fig. 3.7 Electrical System of UV-2550 UV-2450/2550 3-11...
  • Page 40 3.3 Photometric System This page is intentionally left blank. 3-12 UV-2450/2550...

  • Page 41: Chapter 4 Maintenance And Checking

    Replacement of the Light Source ...................... 4-6 4.3.1 Light Source Specifications ....................4-6 4.3.2 Light Source Replacement Procedure.................. 4-6 Replacement of the Fuses......................... 4-9 Cleaning the Unit ..........................4-11 Consumable/Spare Parts List for Maintenance ................4-12 Precaution During Transportation of the Unit .................. 4-13 UV-2450/2550...

  • Page 42: Periodic Maintenance

    4. Select "Peak Pick" from the "Manipulation" menu. Select the tab corresponding to the wavelength check spectrum. The peak wavelength should fall in the range between 655.8 nm and 656.4 nm for UV-2450/2550. 5. Perform the same procedure again for the other characteristic peak of D2 light using: Wavelength Range : 490 (Start) - 480 (End) In this case, the peak wavelength should fall in the range between 485.7 nm and 486.3 nm...

  • Page 43: Troubleshooting

    (dip switch, jumpers, setup, etc.). In the software operation, select "Setting" under the "Instrument" menu. Designate the proper port number under: Optical Bench Serial Port. Establish communications with the optical photometer unit by selecting "Connect" button. Error appear at stages in the initialization (red marks appear) UV-2450/2550...

  • Page 44: Scanning Problems

    Table 4.1 Initialization Errors and Remedial Actions Step from Tables Items to be checked Remedial Action 2.1 or 2.2 1 - 7 (UV-2450) Failure on these items will require service 1 - 8 (UV-2550) by Shimadzu or Shimadzu designated dealers for your location.
  • Page 45 Wavelength setting is not Set the wavelength appropriately. appropriate. Faulty sample Correct sample preparation. preparation Improper cell being used. The glass cell is opaque in the UV region. Use a silica cell. UV-2450/2550...

  • Page 46: Replacement Of The Light Source

    2. Be especially careful when removing and mounting the light source compartment cover so that the back of the cover does not strike the protrusion on the top of the D2 lamp (deuterium lamp). Such a hit could cause a leak in the tube's vacuum. UV-2450/2550...
  • Page 47 4.3 Replacement of the Light Source D2 lamp Wl lamp Fig. 4.2 Interior of light source compartment (UV-2450) Wl lamp D2 lamp Fig. 4.3 Interior of light source compartment (UV-2550) • Procedure for Wl Lamp replacement 1. After allowing the light source cover to cool sufficiently, remove the light source cover (see Fig.
  • Page 48 • Procedure for D2 lamp replacement 1. Open the light source cover and remove the old lamp. 2. Insert a new D2 lamp fully into the socket. (Wear a glove to prevent staining of the glass.) UV-2450/2550...

  • Page 49: Replacement Of The Fuses

    Standard screw driver (minus) Cover of fuse holder Fig. 4.5 Opening the fuse holder cover (4) Pull out the fuse holder, as shown in Fig. 4.6. Cover of fuse holder Fuse Circular plug Fuse holder Fig. 4.6 Interior of fuse holder UV-2450/2550...
  • Page 50 (6) After replacing the fuse, push the fuse holder in so that the arrow on the fuse holder points down. (7) Close the fuse holder cover until you hear its snap shut. NOTE After replacing the fuse, turn ON the power switch and check to see that the until initializes properly. 4-10 UV-2450/2550...

  • Page 51: Cleaning The Unit

    CAUTION Be very careful not to spill water, organic solvent, etc. on the instrument. Spilling liquid on the instrument can cause electric shock, fire, damage or malfunction of the instrument. UV-2450/2550 4-11...

  • Page 52: Consumable/Spare Parts List For Maintenance

    Consumable/Spare Parts List for Maintenance Table 4.3 Consumable Parts for UV-2450/2550 Classification Description Model Part No. Remark Consumable Halogen lamp RJ-5012 / 64604 062-65004-06 Deuterium lamp L-6380 062-65055-05 Maintenance Fuse 5A (100 - 120V) 072-01652-23 Fuse 3.15A (220 - 240V)

  • Page 53: Precaution During Transportation Of The Unit

    Precaution During Transportation of the Unit CAUTION This unit weights about 35 kg. When moving this unit, it must be lifted by two persons firmly holding the positions shown in the diagram. Fig. 4.7 UV-2450/2550 4-13...
  • Page 54 4.7 Precaution During Transportation of the Unit This page is intentionally left blank. 4-14 UV-2450/2550...

  • Page 55: Chapter 5 Specifications

    5 Specifications Chapter 5 Specifications CONTENTS UV-2450/2550 ........................... 5-2 UV-2450/2550...
  • Page 56 Automatic wavelength calibration is possible. Wavelength repeatability ± 0.1 nm Wavelength scanning speed In the case of wavelength setting by: Goto WL command: approx. 3200 nm/min [UV-2450] 2400 nm/min [UV-2550] Pop Up Scan approx.1400 nm/min (with 2 nm sampling interval) FAST approx.550 nm/min MIDDLE approx.210 nm/min These are the speed when scanning...
  • Page 57 50W halogen lamp (long life type 2000H), Deuterium lamp (socket type). Automatic position alignment for maximum sensitivity. Monochromator Aberration corrected Czerny-Turner mounting. High performance blazed holographic grating is used (UV-2450). High performance double blazed holographic grating is used (UV-2550). Detector Photomultiplier R-928 Sample compartment Inside dimensions: 150W ×...
  • Page 58 5.1 UV-2450/2550 Table 5.1 Specifications for UV-2450/2550 (Continued) Data processing Arithmetic calculation of spectrum and constant value Arithmetic calculation between two spectrums Smoothing Differentiation (1st - 4th order) 1/Y Calculation Log Calculation A ↔T% Energy → T% Kubelka-Munk (For R%)

  • Page 59: Chapter 6 Optional Accessories

    6 Optional Accessories Chapter 6 Optional Accessories CONTENTS Optional Cells ............................ 6-2 UV-2450/2550...

  • Page 60: Optional Cells

    10 mm optical path holder (P/N and required sample 206-14334) volume of 50 µl or more required Micro-black cell, with 220-97043-00 Super-micro cell 10 mm optical path and holder (P/N required sample volume of 206-14334) 50 µl or more required UV-2450/2550...
  • Page 61 200-34670-00 1.5 ml 4 × 36 Not required, but For general use, front plate with hole without tubes required Table 6.2 Available Cell Wavelength Ranges Quartz (S cell) 190 - 2500 nm Glass (G cell) 320 - 2500 nm UV-2450/2550...
  • Page 62 6.1 Optional Cells This page is intentionally left blank. UV-2450/2550...
  • Page 63 IXIndex Index UV-2450/2550 IX-1...
  • Page 64 Checking wavelength accuracy ......................4-2 Communications ..........................2-10 Computer RS-232C port ........................4-3 Construction ............................3-1 Consumable Parts for UV-2450/2550 ....................4-12 Consumable/Spare Parts list ....................... 4-12 D2 lamp replacement ..........................4-8 Electrical System of UV-2450 ......................3-10 Electrical System of UV-2550 ......................3-11 Environmental Requirements ........................
  • Page 65 Index Optical Schematics of UV-2450 ......................3-5 Optical Schematics of UV-2550 ......................3-7 Photometer Initialization Failures ......................4-3 Photometric System ..........................3-5 Printer Setup ............................2-10 Recommended optional accessories ......................2-4 Replacement of the Light Source ......................4-6 RS-232C serial cable ..........................4-3 Sample compartment of UV-2450/2550 ....................3-4 Scanning Problems ..........................4-4...
  • Page 66 Index This page is intentionally left blank. IX-4 UV-2450/2550...
  • Page 67 Record of Revision Date Revision Changed Page Description 2/2004 A: Record of Revision 206-94376 is unified into this IM. 1/2006 Note for WEEE Mark was added. 8/2006 Modification in layout Modification in phrases, pointed out by a native checker 8/2008 i, v, vi, 2-13 A: Emergency Operation and Operation at Power Outage...

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